NRAM2_DICDI
ID NRAM2_DICDI Reviewed; 629 AA.
AC Q553K4; Q86H95;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=Natural resistance-associated macrophage protein 2 homolog;
GN Name=nramp2; Synonyms=mntH; ORFNames=DDB_G0275815;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Divalent transition metal (iron and manganese) transporter.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the NRAMP family. {ECO:0000305}.
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DR EMBL; AAFI02000013; EAL69658.1; -; Genomic_DNA.
DR RefSeq; XP_643409.1; XM_638317.1.
DR AlphaFoldDB; Q553K4; -.
DR SMR; Q553K4; -.
DR STRING; 44689.DDB0232114; -.
DR TCDB; 2.A.55.2.10; the metal ion (mn(2+)-iron) transporter (nramp) family.
DR PaxDb; Q553K4; -.
DR EnsemblProtists; EAL69658; EAL69658; DDB_G0275815.
DR GeneID; 8619995; -.
DR KEGG; ddi:DDB_G0275815; -.
DR dictyBase; DDB_G0275815; nrampB.
DR eggNOG; KOG1291; Eukaryota.
DR HOGENOM; CLU_020088_0_1_1; -.
DR InParanoid; Q553K4; -.
DR OMA; AYIDPGS; -.
DR PhylomeDB; Q553K4; -.
DR PRO; PR:Q553K4; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0031164; C:contractile vacuolar membrane; IDA:dictyBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015086; F:cadmium ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015093; F:ferrous iron transmembrane transporter activity; IDA:dictyBase.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0031152; P:aggregation involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0030026; P:cellular manganese ion homeostasis; IBA:GO_Central.
DR GO; GO:0042742; P:defense response to bacterium; IMP:dictyBase.
DR GO; GO:1903988; P:iron ion export across plasma membrane; IDA:dictyBase.
DR GO; GO:0055072; P:iron ion homeostasis; IMP:dictyBase.
DR GO; GO:0006826; P:iron ion transport; IBA:GO_Central.
DR GO; GO:0009617; P:response to bacterium; IDA:dictyBase.
DR HAMAP; MF_00221; NRAMP; 1.
DR InterPro; IPR001046; NRAMP_fam.
DR PANTHER; PTHR11706; PTHR11706; 1.
DR Pfam; PF01566; Nramp; 1.
DR PRINTS; PR00447; NATRESASSCMP.
DR TIGRFAMs; TIGR01197; nramp; 1.
PE 3: Inferred from homology;
KW Cell membrane; Glycoprotein; Manganese; Membrane; Reference proteome;
KW Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..629
FT /note="Natural resistance-associated macrophage protein 2
FT homolog"
FT /id="PRO_0000328223"
FT TOPO_DOM 1..151
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 173..182
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 204..224
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 246..253
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..274
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 275..286
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 287..307
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 308..326
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 348..376
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 398..421
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 422..442
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 443..468
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 469..489
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 490..491
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 492..512
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 513..527
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 528..548
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 549..565
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 566..586
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 587..629
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 50..119
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 50..89
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..108
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 557
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 629 AA; 70404 MW; 7430FE9D224A57BA CRC64;
MNNNNNNKKL NKENLNEIEN WNEIELTDYK KQGLDEGTSS NIYLFESNGN VVNGSIEDSP
KQQQQQQQQQ QQQQQQQQQQ QQQQQNIISS DEEIEDKPFQ DRDSNIGDGS DIDSSGDSID
IENTDYSILV PFEDEENNNG SKSKFSIKKL KSFLGPALFI SVGYMDPGNW ATDLEGGSRF
GYQLMWVLLF SNIMALFLQT LVIKLALVTK NDLAQQCRKE YSKTVNIFLW LILELAIIST
DLAEVIGTAI GLNILFGLPL IAGVAITSLD TLLFLAIQRW GIRKLELLIL LLLSMITMCF
VIELFLSKPI ASEVFSGFVP RLNSDSVMVA TGIVGATTMP HNLFLHGSVV KSRKIPNDRR
KSVIKQAYRY NVIDTVLALN CAFFVNIAIL MLAASVFWKS NIQVTELSEA YRLLTKLMDG
KLAAVLFGLG LFLAGQSSTI TGTMAGQIVM EGFIKLRIKP WLRRFITRLL AIIPAAIVII
VLGDKGTYTL LIISQVLLSI GLPFAVVPLI IFTSSYEIMG EFKNRLSIII INSIIALFII
GLNLATIFQL INDFLHNDSI ISKCLTIIFL IPLSIALCCL LLWLIISKIN FFTNLLSKIF
NNNNNNNNKN IINNNNNYSG NTINNQTIQ
