NRAM3_ARATH
ID NRAM3_ARATH Reviewed; 509 AA.
AC Q9SNV9; O22192;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 11-APR-2003, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Metal transporter Nramp3;
DE Short=AtNramp3;
GN Name=NRAMP3; OrderedLocusNames=At2g23150; ORFNames=F21P24.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=10781110; DOI=10.1073/pnas.97.9.4991;
RA Thomine S., Wang R., Ward J.M., Crawford N.M., Schroeder J.I.;
RT "Cadmium and iron transport by members of a plant metal transporter family
RT in Arabidopsis with homology to Nramp genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:4991-4996(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=cv. Wassilewskija;
RX PubMed=12787249; DOI=10.1046/j.1365-313x.2003.01760.x;
RA Thomine S., Lelievre F., Debarbieux E., Schroeder J.I., Barbier-Brygoo H.;
RT "AtNRAMP3, a multispecific vacuolar metal transporter involved in plant
RT responses to iron deficiency.";
RL Plant J. 34:685-695(2003).
RN [6]
RP FUNCTION.
RC STRAIN=cv. Wassilewskija;
RX PubMed=16270029; DOI=10.1038/sj.emboj.7600864;
RA Lanquar V., Lelievre F., Bolte S., Hames C., Alcon C., Neumann D.,
RA Vansuyt G., Curie C., Schroeder A., Kraemer U., Barbier-Brygoo H.,
RA Thomine S.;
RT "Mobilization of vacuolar iron by AtNRAMP3 and AtNRAMP4 is essential for
RT seed germination on low iron.";
RL EMBO J. 24:4041-4051(2005).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=17151019; DOI=10.1074/mcp.m600250-mcp200;
RA Jaquinod M., Villiers F., Kieffer-Jaquinod S., Hugouvieux V., Bruley C.,
RA Garin J., Bourguignon J.;
RT "A proteomics dissection of Arabidopsis thaliana vacuoles isolated from
RT cell culture.";
RL Mol. Cell. Proteomics 6:394-412(2007).
RN [8]
RP FUNCTION, AND INDUCTION.
RC STRAIN=cv. Wassilewskija;
RX PubMed=19121106; DOI=10.1111/j.1365-313x.2008.03775.x;
RA Segond D., Dellagi A., Lanquar V., Rigault M., Patrit O., Thomine S.,
RA Expert D.;
RT "NRAMP genes function in Arabidopsis thaliana resistance to Erwinia
RT chrysanthemi infection.";
RL Plant J. 58:195-207(2009).
RN [9]
RP FUNCTION.
RX PubMed=20181755; DOI=10.1104/pp.109.150946;
RA Lanquar V., Ramos M.S., Lelievre F., Barbier-Brygoo H., Krieger-Liszkay A.,
RA Kraemer U., Thomine S.;
RT "Export of vacuolar manganese by AtNRAMP3 and AtNRAMP4 is required for
RT optimal photosynthesis and growth under manganese deficiency.";
RL Plant Physiol. 152:1986-1999(2010).
CC -!- FUNCTION: Vacuolar metal transporter involved in intracellular metal
CC homeostasis. Can transport iron (Fe), manganese (Mn) and cadmium (Cd).
CC Regulates metal accumulation under Fe starvation. Acts redundantly with
CC NRAMP4 to mobilize vacuolar Fe and provide sufficient Fe during seed
CC germination. In association with NRAMP4, required for optimal growth
CC and photosynthesis under Mn deficiency. Exports Mn from vacuoles in
CC leaf mesophyll cells, making Mn available for functional photosystem II
CC in chloroplasts. Involved in basal resistance to the bacterial pathogen
CC E.chrysanthemi. {ECO:0000269|PubMed:10781110,
CC ECO:0000269|PubMed:12787249, ECO:0000269|PubMed:16270029,
CC ECO:0000269|PubMed:19121106, ECO:0000269|PubMed:20181755}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:12787249,
CC ECO:0000269|PubMed:17151019}; Multi-pass membrane protein {ECO:0000255,
CC ECO:0000269|PubMed:12787249}.
CC -!- TISSUE SPECIFICITY: Expressed in vascular tissues.
CC {ECO:0000269|PubMed:12787249}.
CC -!- INDUCTION: By iron starvation and infection with the bacterial
CC pathogens P.syringae and E.chrysanthemi. {ECO:0000269|PubMed:10781110,
CC ECO:0000269|PubMed:12787249, ECO:0000269|PubMed:19121106}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC condition, but slightly enhanced resistance of root growth in presence
CC of Cd and increased accumulation of Mn and Zn under Fe starvation.
CC {ECO:0000269|PubMed:10781110, ECO:0000269|PubMed:12787249}.
CC -!- SIMILARITY: Belongs to the NRAMP (TC 2.A.55) family. {ECO:0000305}.
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DR EMBL; AF202539; AAF13278.1; -; mRNA.
DR EMBL; AC002391; AAB87118.1; -; Genomic_DNA.
DR EMBL; AC004401; AAM14929.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07422.1; -; Genomic_DNA.
DR EMBL; AY058203; AAL25615.1; -; mRNA.
DR PIR; T00517; T00517.
DR RefSeq; NP_179896.1; NM_127879.4.
DR AlphaFoldDB; Q9SNV9; -.
DR SMR; Q9SNV9; -.
DR BioGRID; 2200; 4.
DR IntAct; Q9SNV9; 4.
DR STRING; 3702.AT2G23150.1; -.
DR PaxDb; Q9SNV9; -.
DR PRIDE; Q9SNV9; -.
DR ProteomicsDB; 239056; -.
DR EnsemblPlants; AT2G23150.1; AT2G23150.1; AT2G23150.
DR GeneID; 816847; -.
DR Gramene; AT2G23150.1; AT2G23150.1; AT2G23150.
DR KEGG; ath:AT2G23150; -.
DR Araport; AT2G23150; -.
DR TAIR; locus:2045344; AT2G23150.
DR eggNOG; KOG1291; Eukaryota.
DR HOGENOM; CLU_020088_5_1_1; -.
DR InParanoid; Q9SNV9; -.
DR OMA; YDETEKV; -.
DR OrthoDB; 666470at2759; -.
DR PhylomeDB; Q9SNV9; -.
DR PRO; PR:Q9SNV9; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SNV9; baseline and differential.
DR Genevisible; Q9SNV9; AT.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0005774; C:vacuolar membrane; IDA:TAIR.
DR GO; GO:0005773; C:vacuole; HDA:TAIR.
DR GO; GO:0015086; F:cadmium ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0042742; P:defense response to bacterium; IMP:TAIR.
DR GO; GO:0055072; P:iron ion homeostasis; IMP:TAIR.
DR GO; GO:0006828; P:manganese ion transport; IMP:TAIR.
DR GO; GO:2000379; P:positive regulation of reactive oxygen species metabolic process; IGI:TAIR.
DR GO; GO:0009617; P:response to bacterium; IEP:TAIR.
DR GO; GO:0010043; P:response to zinc ion; IEP:TAIR.
DR HAMAP; MF_00221; NRAMP; 1.
DR InterPro; IPR001046; NRAMP_fam.
DR PANTHER; PTHR11706; PTHR11706; 1.
DR Pfam; PF01566; Nramp; 1.
DR PRINTS; PR00447; NATRESASSCMP.
DR TIGRFAMs; TIGR01197; nramp; 1.
PE 1: Evidence at protein level;
KW Ion transport; Iron; Iron transport; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..509
FT /note="Metal transporter Nramp3"
FT /id="PRO_0000212600"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 84..104
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 239..259
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 383..403
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 406..426
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 444..464
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 472..492
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 433
FT /note="G -> S (in Ref. 1; AAF13278)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 509 AA; 56138 MW; 5E65C789317508C8 CRC64;
MPQLENNEPL LINEEEEEET AYDETEKVHI VRNEEEDDLE HGVGCGGAPP FSWKKLWLFT
GPGFLMSIAF LDPGNLEGDL QAGAVAGYSL LWLLMWATAM GLLVQLLSAR LGVATGRHLA
ELCRDEYPTW ARMVLWVMAE LALIGSDIQE VIGSAIAIKI LSNGILPLWA GVVITALDCF
VFLFLENYGI RKLEAVFAVL IATMGVSFAW MFGQAKPSGS ELLIGILVPK LSSRTIQKAV
GVVGCIIMPH NVFLHSALVQ SREVDKRQKY RVQEALNYYT IESTIALFIS FLINLFVTTV
FAKGFYNTDL ANSIGLVNAG QYLQEKYGGG VFPILYIWAI GLLAAGQSST ITGTYAGQFI
MGGFLNFKMK KWLRALITRS CAIIPTIIVA LVFDSSEATL DVLNEWLNVL QSIQIPFALI
PLLCLVSKEQ IMGSFKIGPL YKTIAWLVAA LVIMINGYLL LEFFSNEVSG IVYTGFVTLF
TASYGAFILY LIARGITFTP WPFKAESSH
