NRAM4_ARATH
ID NRAM4_ARATH Reviewed; 512 AA.
AC Q9FN18; Q0WWH0; Q9SNV8;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Metal transporter Nramp4;
DE Short=AtNramp4;
GN Name=NRAMP4; OrderedLocusNames=At5g67330; ORFNames=K8K14.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INDUCTION.
RC STRAIN=cv. Columbia;
RX PubMed=10781110; DOI=10.1073/pnas.97.9.4991;
RA Thomine S., Wang R., Ward J.M., Crawford N.M., Schroeder J.I.;
RT "Cadmium and iron transport by members of a plant metal transporter family
RT in Arabidopsis with homology to Nramp genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:4991-4996(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-512.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=cv. Wassilewskija;
RX PubMed=16270029; DOI=10.1038/sj.emboj.7600864;
RA Lanquar V., Lelievre F., Bolte S., Hames C., Alcon C., Neumann D.,
RA Vansuyt G., Curie C., Schroeder A., Kraemer U., Barbier-Brygoo H.,
RA Thomine S.;
RT "Mobilization of vacuolar iron by AtNRAMP3 and AtNRAMP4 is essential for
RT seed germination on low iron.";
RL EMBO J. 24:4041-4051(2005).
RN [7]
RP FUNCTION.
RX PubMed=20181755; DOI=10.1104/pp.109.150946;
RA Lanquar V., Ramos M.S., Lelievre F., Barbier-Brygoo H., Krieger-Liszkay A.,
RA Kraemer U., Thomine S.;
RT "Export of vacuolar manganese by AtNRAMP3 and AtNRAMP4 is required for
RT optimal photosynthesis and growth under manganese deficiency.";
RL Plant Physiol. 152:1986-1999(2010).
CC -!- FUNCTION: Vacuolar metal transporter involved in intracellular metal
CC homeostasis. Can transport iron (Fe), manganese (Mn) and cadmium (Cd).
CC Regulates metal accumulation under Fe starvation. Acts redundantly with
CC NRAMP3 to mobilize vacuolar Fe and provide sufficient Fe during seed
CC germination. In association with NRAMP3, required for optimal growth
CC and photosynthesis under Mn deficiency. Exports Mn from vacuoles in
CC leaf mesophyll cells, making Mn available for functional photosystem II
CC in chloroplasts. {ECO:0000269|PubMed:10781110,
CC ECO:0000269|PubMed:16270029, ECO:0000269|PubMed:20181755}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:16270029};
CC Multi-pass membrane protein {ECO:0000269|PubMed:16270029}.
CC -!- TISSUE SPECIFICITY: Expressed in vascular tissues.
CC {ECO:0000269|PubMed:16270029}.
CC -!- INDUCTION: By iron starvation. {ECO:0000269|PubMed:10781110,
CC ECO:0000269|PubMed:16270029}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC condition, but slightly enhanced resistance of root growth in presence
CC of Cd and increased accumulation of Mn and Zn under Fe starvation.
CC {ECO:0000269|PubMed:16270029}.
CC -!- SIMILARITY: Belongs to the NRAMP (TC 2.A.55) family. {ECO:0000305}.
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DR EMBL; AF202540; AAF13279.1; -; mRNA.
DR EMBL; AB007645; BAB09018.1; -; Genomic_DNA.
DR EMBL; CP002688; AED98329.1; -; Genomic_DNA.
DR EMBL; AY136467; AAM97132.1; -; mRNA.
DR EMBL; BT003406; AAO30069.1; -; mRNA.
DR EMBL; AK226381; BAE98528.1; -; mRNA.
DR RefSeq; NP_201534.1; NM_126133.4.
DR AlphaFoldDB; Q9FN18; -.
DR SMR; Q9FN18; -.
DR BioGRID; 22110; 7.
DR IntAct; Q9FN18; 5.
DR STRING; 3702.AT5G67330.1; -.
DR TCDB; 2.A.55.2.18; the metal ion (mn(2+)-iron) transporter (nramp) family.
DR PaxDb; Q9FN18; -.
DR PRIDE; Q9FN18; -.
DR ProteomicsDB; 239057; -.
DR EnsemblPlants; AT5G67330.1; AT5G67330.1; AT5G67330.
DR GeneID; 836868; -.
DR Gramene; AT5G67330.1; AT5G67330.1; AT5G67330.
DR KEGG; ath:AT5G67330; -.
DR Araport; AT5G67330; -.
DR TAIR; locus:2158242; AT5G67330.
DR eggNOG; KOG1291; Eukaryota.
DR HOGENOM; CLU_020088_5_1_1; -.
DR InParanoid; Q9FN18; -.
DR OMA; SHKEPQR; -.
DR OrthoDB; 666470at2759; -.
DR PhylomeDB; Q9FN18; -.
DR PRO; PR:Q9FN18; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FN18; baseline and differential.
DR Genevisible; Q9FN18; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0005774; C:vacuolar membrane; IDA:TAIR.
DR GO; GO:0005773; C:vacuole; HDA:TAIR.
DR GO; GO:0015086; F:cadmium ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0042742; P:defense response to bacterium; IMP:TAIR.
DR GO; GO:0055072; P:iron ion homeostasis; IMP:TAIR.
DR GO; GO:0006828; P:manganese ion transport; IMP:TAIR.
DR GO; GO:2000379; P:positive regulation of reactive oxygen species metabolic process; IGI:TAIR.
DR GO; GO:0010039; P:response to iron ion; IEP:TAIR.
DR GO; GO:0009624; P:response to nematode; HEP:TAIR.
DR HAMAP; MF_00221; NRAMP; 1.
DR InterPro; IPR001046; NRAMP_fam.
DR PANTHER; PTHR11706; PTHR11706; 1.
DR Pfam; PF01566; Nramp; 1.
DR PRINTS; PR00447; NATRESASSCMP.
DR TIGRFAMs; TIGR01197; nramp; 1.
PE 2: Evidence at transcript level;
KW Ion transport; Iron; Iron transport; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..512
FT /note="Metal transporter Nramp4"
FT /id="PRO_0000212601"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..149
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 235..255
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 277..297
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 323..343
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 371..391
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 402..422
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 440..460
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 468..488
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 246
FT /note="H -> N (in Ref. 5; BAE98528)"
FT /evidence="ECO:0000305"
FT CONFLICT 467
FT /note="L -> V (in Ref. 1; AAF13279)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 512 AA; 56385 MW; 89F2CE2359DF3324 CRC64;
MSETDRERPL LASEERAYEE TEKVLIVGID EEEDADYDDD PGNSPKFSWK KLWLFTGPGF
LMSIAFLDPG NLESDLQAGA IAGYSLIWLL MWATAIGLLI QLLSARLGVA TGRHLAELCR
EEYPTWARMV LWIMAEIALI GADIQEVIGS AIAIKILSNG LVPLWAGVVI TALDCFIFLF
LENYGIRKLE AVFAILIATM ALAFAWMFGQ TKPSGTELLV GALVPKLSSR TIKQAVGIVG
CIIMPHNVFL HSALVQSREV DPKKRFRVKE ALKYYSIEST GALAVSFIIN VFVTTVFAKS
FYGTEIADTI GLANAGQYLQ DKYGGGFFPI LYIWAIGVLA AGQSSTITGT YAGQFIMGGF
LNLKMKKWVR ALITRSCAII PTMIVALVFD SSDSMLDELN EWLNVLQSVQ IPFAVIPLLC
LVSNEQIMGS FKIQPLVQTI SWIVAALVIA INGYLMVDFF SGAATNLILL VPVIIFAIAY
VVFVLYLISR GLTYTPWQLV ASSHKEPQRD DE
