NRAM4_ORYSJ
ID NRAM4_ORYSJ Reviewed; 550 AA.
AC Q5QN13; A0A0P0V3B2; B7EF59; B7EKG9; Q5QN12;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 1.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Metal transporter Nramp4;
GN Name=NRAMP4; OrderedLocusNames=Os01g0503400, LOC_Os01g31870;
GN ORFNames=OsJ_01931, P0507H06.36-1, P0507H06.36-2;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Probable metal transporter. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q5QN13-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5QN13-2; Sequence=VSP_040698, VSP_040699;
CC Name=3;
CC IsoId=Q5QN13-3; Sequence=VSP_040695, VSP_040698, VSP_040699;
CC Name=4;
CC IsoId=Q5QN13-4; Sequence=VSP_040696, VSP_040697;
CC -!- SIMILARITY: Belongs to the NRAMP (TC 2.A.55) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAG91007.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AP003144; BAD73194.1; -; Genomic_DNA.
DR EMBL; AP003144; BAD73195.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF05053.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS72334.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS72335.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS72336.1; -; Genomic_DNA.
DR EMBL; CM000138; EEE54653.1; -; Genomic_DNA.
DR EMBL; AK068118; BAG90764.1; -; mRNA.
DR EMBL; AK068641; BAG91006.1; -; mRNA.
DR EMBL; AK068641; BAG91007.1; ALT_INIT; mRNA.
DR EMBL; AK072201; BAG92866.1; -; mRNA.
DR EMBL; AK122122; BAH00803.1; -; mRNA.
DR RefSeq; XP_015620405.1; XM_015764919.1. [Q5QN13-1]
DR RefSeq; XP_015620414.1; XM_015764928.1.
DR AlphaFoldDB; Q5QN13; -.
DR SMR; Q5QN13; -.
DR STRING; 4530.OS01T0503400-03; -.
DR PaxDb; Q5QN13; -.
DR PRIDE; Q5QN13; -.
DR EnsemblPlants; Os01t0503400-03; Os01t0503400-03; Os01g0503400. [Q5QN13-1]
DR EnsemblPlants; Os01t0503400-04; Os01t0503400-04; Os01g0503400. [Q5QN13-4]
DR EnsemblPlants; Os01t0503400-05; Os01t0503400-05; Os01g0503400. [Q5QN13-2]
DR GeneID; 4324486; -.
DR Gramene; Os01t0503400-03; Os01t0503400-03; Os01g0503400. [Q5QN13-1]
DR Gramene; Os01t0503400-04; Os01t0503400-04; Os01g0503400. [Q5QN13-4]
DR Gramene; Os01t0503400-05; Os01t0503400-05; Os01g0503400. [Q5QN13-2]
DR KEGG; osa:4324486; -.
DR eggNOG; KOG1291; Eukaryota.
DR InParanoid; Q5QN13; -.
DR OMA; NIYFFCT; -.
DR OrthoDB; 666470at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000007752; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR ExpressionAtlas; Q5QN13; baseline and differential.
DR Genevisible; Q5QN13; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015086; F:cadmium ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR HAMAP; MF_00221; NRAMP; 1.
DR InterPro; IPR001046; NRAMP_fam.
DR PANTHER; PTHR11706; PTHR11706; 1.
DR Pfam; PF01566; Nramp; 1.
DR PRINTS; PR00447; NATRESASSCMP.
DR TIGRFAMs; TIGR01197; nramp; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Ion transport; Iron; Iron transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..550
FT /note="Metal transporter Nramp4"
FT /id="PRO_0000405569"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..312
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 388..408
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 416..436
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 457..477
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 492..512
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..139
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_040695"
FT VAR_SEQ 204..210
FT /note="ARKMEFT -> VRIFLCL (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_040696"
FT VAR_SEQ 211..550
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_040697"
FT VAR_SEQ 380..390
FT /note="GFSGMRKCIIY -> VHLPNYWSRLD (in isoform 2 and isoform
FT 3)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_040698"
FT VAR_SEQ 391..550
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_040699"
SQ SEQUENCE 550 AA; 59057 MW; B3CFF24C6D05FAF8 CRC64;
MEEGAKIGRE HEQQQQQHGR VNGSGRVAAV GGGSGGGGDE IEIEVAAAAG ASPSRQHGGL
HGDVQAPTWK RFLAHVGPGF VISIAYLDPS NLQTDLVAGS SHRYSLLWVL LFGFIFVLTV
QSLAANLGII TGRHLAELCM GEYPKYVKYC LWLLAELGVI AATIPGVLGT ALAYNMLLHI
PFWAGVLACG ACTFLILGLQ GYGARKMEFT ISVLMLVMAT CFFMELGKVN PPAGGVIEGL
FIPRPKGDYS TSDAVAMFGS LVVPHNLFLH SSLVLTRKMP YTSKGRKDAS TFFLLENALA
LFIALLVNVA IVSISGTICA NNLSFADTST CSSLTLNSTY VLLKNILGKS SSTVYGVALL
VSGQSCMVAT SYAGQYIMQG FSGMRKCIIY LVAPCFTLLP SLIICSIGGT LRVHRIINIA
AIVLSFVLPF ALIPLIKFSS SCTNIGPYKN ATSIIRIAWI LSLVIIGINI YFFCTSFVAW
LVHSDLPRVV NAIISSLVFP FMAAYIAALI YLAFRKVNLS DPFPTNSVSG EIEVQHIQIQ
EKQEDLGVHL
