NRAM6_ARATH
ID NRAM6_ARATH Reviewed; 527 AA.
AC Q9S9N8; Q84N36; Q9C5V8;
DT 23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 23-APR-2003, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Metal transporter Nramp6;
DE Short=AtNramp6;
GN Name=NRAMP6; OrderedLocusNames=At1g15960; ORFNames=T24D18.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Landsberg erecta;
RA Pittman J.K., Hall J.L., Williams L.E.;
RT "Identification of Nramp6, a putative heavy metal transporter in
RT Arabidopsis thaliana.";
RL Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=19545236; DOI=10.1042/bj20090655;
RA Cailliatte R., Lapeyre B., Briat J.F., Mari S., Curie C.;
RT "The NRAMP6 metal transporter contributes to cadmium toxicity.";
RL Biochem. J. 422:217-228(2009).
CC -!- FUNCTION: Probable intracellular cadmium (Cd) transporter that
CC participates in the distribution or availability of Cd within the cell.
CC {ECO:0000269|PubMed:19545236}.
CC -!- SUBCELLULAR LOCATION: Endomembrane system
CC {ECO:0000305|PubMed:19545236}; Multi-pass membrane protein
CC {ECO:0000305|PubMed:19545236}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=Nramp6a;
CC IsoId=Q9S9N8-1; Sequence=Displayed;
CC Name=2; Synonyms=Nramp6b;
CC IsoId=Q9S9N8-2; Sequence=VSP_040700, VSP_040701;
CC -!- TISSUE SPECIFICITY: Expressed in the vascular bundles of shoots,
CC cotyledons, young leaves, sepals and petals, at the top of the flower
CC stem and in the style. Expressed in the peduncle of developing siliques
CC as well as in the septum and the funiculi.
CC {ECO:0000269|PubMed:19545236}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC condition, but in presence of Cd, increased tolerance to Cd toxicity.
CC {ECO:0000269|PubMed:19545236}.
CC -!- SIMILARITY: Belongs to the NRAMP (TC 2.A.55) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF18493.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AJ291831; CAC28123.1; -; mRNA.
DR EMBL; AY263397; AAP22982.1; -; mRNA.
DR EMBL; AC010924; AAF18493.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29390.1; -; Genomic_DNA.
DR PIR; B86294; B86294.
DR RefSeq; NP_173048.3; NM_101464.4. [Q9S9N8-1]
DR AlphaFoldDB; Q9S9N8; -.
DR SMR; Q9S9N8; -.
DR STRING; 3702.AT1G15960.1; -.
DR PaxDb; Q9S9N8; -.
DR PRIDE; Q9S9N8; -.
DR ProteomicsDB; 239059; -. [Q9S9N8-1]
DR EnsemblPlants; AT1G15960.1; AT1G15960.1; AT1G15960. [Q9S9N8-1]
DR GeneID; 838166; -.
DR Gramene; AT1G15960.1; AT1G15960.1; AT1G15960. [Q9S9N8-1]
DR KEGG; ath:AT1G15960; -.
DR Araport; AT1G15960; -.
DR TAIR; locus:2200437; AT1G15960.
DR eggNOG; KOG1291; Eukaryota.
DR HOGENOM; CLU_020088_0_1_1; -.
DR InParanoid; Q9S9N8; -.
DR OMA; QCLCAKL; -.
DR OrthoDB; 666470at2759; -.
DR PhylomeDB; Q9S9N8; -.
DR PRO; PR:Q9S9N8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9S9N8; baseline and differential.
DR Genevisible; Q9S9N8; AT.
DR GO; GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015086; F:cadmium ion transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0070574; P:cadmium ion transmembrane transport; IDA:UniProtKB.
DR GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR HAMAP; MF_00221; NRAMP; 1.
DR InterPro; IPR001046; NRAMP_fam.
DR PANTHER; PTHR11706; PTHR11706; 1.
DR Pfam; PF01566; Nramp; 1.
DR PRINTS; PR00447; NATRESASSCMP.
DR TIGRFAMs; TIGR01197; nramp; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Ion transport; Iron; Iron transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..527
FT /note="Metal transporter Nramp6"
FT /id="PRO_0000212603"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..341
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 364..384
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 385..405
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 427..447
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 458..478
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 170..180
FT /note="IRKLEFLIAFL -> VHLLSFVYYGF (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_040700"
FT VAR_SEQ 181..527
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_040701"
SQ SEQUENCE 527 AA; 57245 MW; 88608331CB334B14 CRC64;
MAAETASGSN RSISNSPLIE NSDSNQILVP EKKSWKNFFS YLGPGFLVSI AYIDPGNFET
DLQSGAQYKY ELLWIILVAS CAALVIQSLA ANLGVVTGKH LAEHCRAEYS KVPNFMLWVV
AEIAVVACDI PEVIGTAFAL NMLFNIPVWI GVLLTGLSTL ILLALQQYGI RKLEFLIAFL
VFTIALCFFV ELHYSKPDPK EVLYGLFVPQ LKGNGATGLA ISLLGAMVMP HNLFLHSALV
LSRKIPRSVT GIKEACRYYL IESGLALMVA FLINVSVISV SGAVCNASDL SPEDRASCQD
LDLNKASFLL RNVVGKWSSK LFAIALLASG QSSTITGTYA GQYVMQGFLD LRLEPWLRNF
LTRCLAIIPS LIVALIGGSA GAGKLIIIAS MILSFELPFA LVPLLKFTSS KTKMGSHANS
LVISSVTWII GGLIMGINIY YLVSSFIKLL LHSHMNLVAI VFLGVLGFSG IATYLAAISY
LVLRKNRESS STHFLDFSNS QTEETLPRED IANMQLPNRV AVIGDLN
