NRAT1_ORYSJ
ID NRAT1_ORYSJ Reviewed; 545 AA.
AC Q6ZG85; A0A0P0VEB7;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Metal transporter NRAT1;
DE AltName: Full=Protein NRAMP ALUMINUM TRANSPORTER 1;
GN Name=NRAT1; OrderedLocusNames=Os02g0131800, LOC_Os02g03900;
GN ORFNames=OJ1007_D04.24, OsJ_05257;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, INDUCTION BY ALUMINUM, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Nipponbare;
RX PubMed=20937890; DOI=10.1073/pnas.1004949107;
RA Xia J., Yamaji N., Kasai T., Ma J.F.;
RT "Plasma membrane-localized transporter for aluminum in rice.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:18381-18385(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Metal transporter that transports the trivalent cation
CC aluminum (Al(3+)), but does not seem to transport divalent cations such
CC as iron (Fe(2+)), manganese (Mg(2+)) or Cadmium (Cd(2+)). Involved in
CC Al tolerance by taking up Al in root cells, where it is detoxified by
CC chelation with organic acid anions and sequestration into the vacuoles.
CC {ECO:0000269|PubMed:20937890}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20937890};
CC Multi-pass membrane protein {ECO:0000269|PubMed:20937890}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in roots.
CC {ECO:0000269|PubMed:20937890}.
CC -!- INDUCTION: By Al in roots. Positively regulated by ART1.
CC {ECO:0000269|PubMed:20937890}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC condition, but after exposure to Al, decreased levels of Al in root-
CC cell sap, increased Al content in the cell wall and increased
CC inhibition of root growth. {ECO:0000269|PubMed:20937890}.
CC -!- SIMILARITY: Belongs to the NRAMP (TC 2.A.55) family. {ECO:0000305}.
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DR EMBL; AB525887; BAJ22943.1; -; mRNA.
DR EMBL; AP004150; BAD07752.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF07708.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS76823.1; -; Genomic_DNA.
DR EMBL; CM000139; EEE56244.1; -; Genomic_DNA.
DR RefSeq; XP_015625418.1; XM_015769932.1.
DR AlphaFoldDB; Q6ZG85; -.
DR SMR; Q6ZG85; -.
DR STRING; 4530.OS02T0131800-01; -.
DR TCDB; 2.A.55.2.5; the metal ion (mn(2+)-iron) transporter (nramp) family.
DR PaxDb; Q6ZG85; -.
DR PRIDE; Q6ZG85; -.
DR EnsemblPlants; Os02t0131800-01; Os02t0131800-01; Os02g0131800.
DR GeneID; 4328200; -.
DR Gramene; Os02t0131800-01; Os02t0131800-01; Os02g0131800.
DR KEGG; osa:4328200; -.
DR eggNOG; KOG1291; Eukaryota.
DR HOGENOM; CLU_020088_0_1_1; -.
DR InParanoid; Q6ZG85; -.
DR OMA; HITLLTM; -.
DR OrthoDB; 666470at2759; -.
DR PlantReactome; R-OSA-9639136; Response to Aluminum stress.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6ZG85; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0015083; F:aluminum ion transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0046873; F:metal ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015690; P:aluminum cation transport; IMP:UniProtKB.
DR GO; GO:0010044; P:response to aluminum ion; IMP:UniProtKB.
DR HAMAP; MF_00221; NRAMP; 1.
DR InterPro; IPR001046; NRAMP_fam.
DR PANTHER; PTHR11706; PTHR11706; 1.
DR Pfam; PF01566; Nramp; 1.
DR PRINTS; PR00447; NATRESASSCMP.
DR TIGRFAMs; TIGR01197; nramp; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..545
FT /note="Metal transporter NRAT1"
FT /id="PRO_0000405571"
FT TRANSMEM 51..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 84..104
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 188..208
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 278..298
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 333..353
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 373..395
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 398..418
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 437..457
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 474..494
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 516..545
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 545 AA; 59245 MW; 25C172D17CA25271 CRC64;
MEGTGEMREV GRETLHGGVV QSVSETDEYK EKTIDSEKDG QFRVQPRWRK FLAHVGPGAL
VAIGFLDPSN LETDMQAGAD FKYELLWVIL VGMVFALLIQ TLAANLGVKT GRHLAELCRE
EYPHYVNIFL WIIAELAVIS DDIPEVLGTA FAFNILLKIP VWAGVILTVF STLLLLGVQR
FGARKLEFII AAFMFTMAAC FFGELSYLRP SAGEVVKGMF VPSLQGKGAA ANAIALFGAI
ITPYNLFLHS ALVLSRKTPR SDKSIRAACR YFLIECSLAF IVAFLINVSV VVVAGSICNA
NNLSPADANT CGDLTLQSTP LLLRNVLGRS SSVVYAVALL ASGQSTTISC TFAGQVIMQG
FLDMKMKNWV RNLITRVIAI APSLIVSIVS GPSGAGKLII LSSMILSFEL PFALIPLLKF
CNSSKKVGPL KESIYTVVIA WILSFALIVV NTYFLVWTYV DWLVHNNLPK YANGLISVVV
FALMAAYLVA VVYLTFRKDT VATYVPVPER AQAQVEAGGT PVVDASAADE DQPAPYRKDL
ADASM
