NRBP2_HUMAN
ID NRBP2_HUMAN Reviewed; 501 AA.
AC Q9NSY0; B4DF59; Q2HIX8; Q597G7; Q8NCX8; Q8WYS5;
DT 07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 15-JUN-2010, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Nuclear receptor-binding protein 2;
DE AltName: Full=Transformation-related gene 16 protein;
DE Short=TRG-16;
GN Name=NRBP2 {ECO:0000312|HGNC:HGNC:19339}; ORFNames=PP9320, TRG16;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAQ18040.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RA Kim J.W.;
RT "Identification of a human transforming gene.";
RL Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Cerebellum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAL55883.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15498874; DOI=10.1073/pnas.0404089101;
RA Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X.,
RA Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.,
RA Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.;
RT "Large-scale cDNA transfection screening for genes related to cancer
RT development and progression.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 208-501 (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K.,
RA Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA Platzer M., Shimizu N., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 50-501 (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7] {ECO:0000305, ECO:0000312|EMBL:AAQ18040.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 149-501 (ISOFORM 1).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-411, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: May regulate apoptosis of neural progenitor cells during
CC their differentiation. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q9NSY0-3; Sequence=Displayed;
CC Name=2 {ECO:0000269|PubMed:15498874, ECO:0000269|Ref.1};
CC IsoId=Q9NSY0-2; Sequence=VSP_039308, VSP_039309;
CC Name=3;
CC IsoId=Q9NSY0-4; Sequence=VSP_039308, VSP_039309, VSP_039310;
CC Name=4;
CC IsoId=Q9NSY0-5; Sequence=VSP_039311, VSP_039312, VSP_039313;
CC -!- DOMAIN: The protein kinase domain is predicted to be catalytically
CC inactive.
CC -!- MISCELLANEOUS: [Isoform 4]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAI13874.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
CC Sequence=CAB70864.1; Type=Miscellaneous discrepancy; Note=Wrong choice of CDS.; Evidence={ECO:0000305};
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DR EMBL; AY277601; AAQ18040.1; -; mRNA.
DR EMBL; AK293943; BAG57320.1; -; mRNA.
DR EMBL; AF318376; AAL55883.1; -; mRNA.
DR EMBL; AL137662; CAB70864.1; ALT_SEQ; mRNA.
DR EMBL; AL834530; CAD39186.1; -; mRNA.
DR EMBL; AC105049; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC113873; AAI13874.1; ALT_SEQ; mRNA.
DR EMBL; CR457350; CAG33631.1; -; mRNA.
DR CCDS; CCDS34959.2; -. [Q9NSY0-3]
DR PIR; T46491; T46491.
DR RefSeq; NP_848659.2; NM_178564.3. [Q9NSY0-3]
DR AlphaFoldDB; Q9NSY0; -.
DR SMR; Q9NSY0; -.
DR BioGRID; 131044; 13.
DR IntAct; Q9NSY0; 60.
DR STRING; 9606.ENSP00000414055; -.
DR iPTMnet; Q9NSY0; -.
DR PhosphoSitePlus; Q9NSY0; -.
DR BioMuta; NRBP2; -.
DR DMDM; 298286835; -.
DR EPD; Q9NSY0; -.
DR jPOST; Q9NSY0; -.
DR MassIVE; Q9NSY0; -.
DR MaxQB; Q9NSY0; -.
DR PaxDb; Q9NSY0; -.
DR PeptideAtlas; Q9NSY0; -.
DR PRIDE; Q9NSY0; -.
DR ProteomicsDB; 82590; -. [Q9NSY0-3]
DR ProteomicsDB; 82591; -. [Q9NSY0-2]
DR ProteomicsDB; 82592; -. [Q9NSY0-4]
DR ProteomicsDB; 82593; -. [Q9NSY0-5]
DR Antibodypedia; 28122; 205 antibodies from 26 providers.
DR DNASU; 340371; -.
DR Ensembl; ENST00000442628.7; ENSP00000414055.2; ENSG00000185189.18. [Q9NSY0-3]
DR GeneID; 340371; -.
DR KEGG; hsa:340371; -.
DR MANE-Select; ENST00000442628.7; ENSP00000414055.2; NM_178564.4; NP_848659.2.
DR UCSC; uc011lkt.3; human. [Q9NSY0-3]
DR CTD; 340371; -.
DR DisGeNET; 340371; -.
DR GeneCards; NRBP2; -.
DR HGNC; HGNC:19339; NRBP2.
DR HPA; ENSG00000185189; Low tissue specificity.
DR MIM; 615563; gene.
DR neXtProt; NX_Q9NSY0; -.
DR OpenTargets; ENSG00000185189; -.
DR PharmGKB; PA134891143; -.
DR VEuPathDB; HostDB:ENSG00000185189; -.
DR eggNOG; KOG1266; Eukaryota.
DR GeneTree; ENSGT00940000160430; -.
DR HOGENOM; CLU_024273_0_0_1; -.
DR InParanoid; Q9NSY0; -.
DR OMA; KVFKAHE; -.
DR OrthoDB; 695382at2759; -.
DR PhylomeDB; Q9NSY0; -.
DR TreeFam; TF315519; -.
DR PathwayCommons; Q9NSY0; -.
DR SignaLink; Q9NSY0; -.
DR BioGRID-ORCS; 340371; 15 hits in 1112 CRISPR screens.
DR ChiTaRS; NRBP2; human.
DR GenomeRNAi; 340371; -.
DR Pharos; Q9NSY0; Tdark.
DR PRO; PR:Q9NSY0; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q9NSY0; protein.
DR Bgee; ENSG00000185189; Expressed in cerebellar vermis and 98 other tissues.
DR ExpressionAtlas; Q9NSY0; baseline and differential.
DR Genevisible; Q9NSY0; HS.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR GO; GO:0016242; P:negative regulation of macroautophagy; IMP:BHF-UCL.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; ISS:UniProtKB.
DR GO; GO:0030182; P:neuron differentiation; ISS:UniProtKB.
DR GO; GO:0006468; P:protein phosphorylation; IBA:GO_Central.
DR CDD; cd14035; PK_MADML; 1.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR042697; NRBP2_PK.
DR InterPro; IPR000719; Prot_kinase_dom.
DR Pfam; PF00069; Pkinase; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Neurogenesis; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..501
FT /note="Nuclear receptor-binding protein 2"
FT /id="PRO_0000225608"
FT DOMAIN 38..306
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 409
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q91V36"
FT MOD_RES 411
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 1..243
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:15498874, ECO:0000303|Ref.1"
FT /id="VSP_039308"
FT VAR_SEQ 1..48
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_039311"
FT VAR_SEQ 149..171
FT /note="AWKRWCTQILSALSFLHACSPPI -> VWGAGWGSHGDRTGLGQPRGLGW
FT (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_039312"
FT VAR_SEQ 172..501
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_039313"
FT VAR_SEQ 244..280
FT /note="MCALEMAVLEIQTNGDTRVTEEAIARARHSLSDPNMR -> MVLQSQNPAVL
FT KLRGPGPGLAAAAGTPPYGHPCMSCVSLRRDMGNSGATSPRTAGPEQRALAHHQPLLPV
FT PQ (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:15498874, ECO:0000303|Ref.1"
FT /id="VSP_039309"
FT VAR_SEQ 461..501
FT /note="TDSAQDLASELVHYGFLHEDDRMKLAAFLESTFLKYRGTQA -> SRLGRGL
FT GRRAVTPGGGWAAASCLALRHCLSGQRPGPRLGARALWLPPRGRPDEAGRLPGEHLPQV
FT PWDPGL (in isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_039310"
FT CONFLICT Q9NSY0-4:10
FT /note="V -> A (in Ref. 1; AAQ18040)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 501 AA; 57803 MW; B3F246DBA7E2AC6C CRC64;
MAAPEPAPRR ARERERERED ESEDESDILE ESPCGRWQKR REQVNQGNMP GLQSTFLAMD
TEEGVEVVWN ELHFGDRKAF AAHEEKIQTV FEQLVLVDHP NIVKLHKYWL DTSEACARVI
FITEYVSSGS LKQFLKKTKK NHKAMNARAW KRWCTQILSA LSFLHACSPP IIHGNLTSDT
IFIQHNGLIK IGSVWHRIFS NALPDDLRSP IRAEREELRN LHFFPPEYGE VADGTAVDIF
SFGMCALEMA VLEIQTNGDT RVTEEAIARA RHSLSDPNMR EFILCCLARD PARRPSAHSL
LFHRVLFEVH SLKLLAAHCF IQHQYLMPEN VVEEKTKAMD LHAVLAELPR PRRPPLQWRY
SEVSFMELDK FLEDVRNGIY PLMNFAATRP LGLPRVLAPP PEEVQKAKTP TPEPFDSETR
KVIQMQCNLE RSEDKARWHL TLLLVLEDRL HRQLTYDLLP TDSAQDLASE LVHYGFLHED
DRMKLAAFLE STFLKYRGTQ A
