NRBP_DROME
ID NRBP_DROME Reviewed; 637 AA.
AC Q9Y0Y6;
DT 23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Nuclear receptor-binding protein homolog;
DE AltName: Full=MLF1-adaptor molecule;
GN Name=Madm {ECO:0000312|EMBL:AAF51961.1, ECO:0000312|FlyBase:FBgn0027497};
GN ORFNames=CG1098;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|EMBL:AAF51961.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAF51961.1}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAD38665.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R {ECO:0000269|PubMed:10731137};
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [4] {ECO:0000305}
RP IDENTIFICATION.
RX PubMed=12176995; DOI=10.1074/jbc.m206041200;
RA Lim R., Winteringham L.N., Williams J.H., McCulloch R.K., Ingley E.,
RA Tiao J.Y.-H., Lalonde J.-P., Tsai S., Tilbrook P.A., Sun Y., Wu X.,
RA Morris S.W., Klinken S.P.;
RT "MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3
RT binding site of myeloid leukemia factor 1.";
RL J. Biol. Chem. 277:40997-41008(2002).
RN [5] {ECO:0000305}
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-473; SER-479; SER-482 AND
RP THR-484, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo {ECO:0000269|PubMed:18327897};
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: May play a role in subcellular trafficking between the
CC endoplasmic reticulum and Golgi apparatus. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cell cortex
CC {ECO:0000250|UniProtKB:Q9UHY1}.
CC -!- DOMAIN: The protein kinase domain is predicted to be catalytically
CC inactive. {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
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DR EMBL; AE014297; AAF51961.1; -; Genomic_DNA.
DR EMBL; AF145690; AAD38665.1; -; mRNA.
DR RefSeq; NP_001303420.1; NM_001316491.1.
DR RefSeq; NP_649581.1; NM_141324.3.
DR AlphaFoldDB; Q9Y0Y6; -.
DR SMR; Q9Y0Y6; -.
DR BioGRID; 65915; 8.
DR STRING; 7227.FBpp0078371; -.
DR iPTMnet; Q9Y0Y6; -.
DR PaxDb; Q9Y0Y6; -.
DR PRIDE; Q9Y0Y6; -.
DR DNASU; 40710; -.
DR EnsemblMetazoa; FBtr0078722; FBpp0078371; FBgn0027497.
DR EnsemblMetazoa; FBtr0347493; FBpp0312573; FBgn0027497.
DR GeneID; 40710; -.
DR KEGG; dme:Dmel_CG1098; -.
DR UCSC; CG1098-RA; d. melanogaster.
DR CTD; 40710; -.
DR FlyBase; FBgn0027497; Madm.
DR VEuPathDB; VectorBase:FBgn0027497; -.
DR eggNOG; KOG1266; Eukaryota.
DR GeneTree; ENSGT00940000168773; -.
DR HOGENOM; CLU_024273_0_0_1; -.
DR InParanoid; Q9Y0Y6; -.
DR OMA; SWRRWCT; -.
DR OrthoDB; 695382at2759; -.
DR PhylomeDB; Q9Y0Y6; -.
DR Reactome; R-DME-383280; Nuclear Receptor transcription pathway.
DR BioGRID-ORCS; 40710; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 40710; -.
DR PRO; PR:Q9Y0Y6; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0027497; Expressed in spermathecum and 21 other tissues.
DR ExpressionAtlas; Q9Y0Y6; baseline and differential.
DR Genevisible; Q9Y0Y6; DM.
DR GO; GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:FlyBase.
DR GO; GO:0012505; C:endomembrane system; ISS:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; IDA:FlyBase.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; ISS:UniProtKB.
DR GO; GO:0036335; P:intestinal stem cell homeostasis; IMP:FlyBase.
DR GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR GO; GO:0006468; P:protein phosphorylation; IBA:GO_Central.
DR GO; GO:0009306; P:protein secretion; IMP:FlyBase.
DR GO; GO:0042127; P:regulation of cell population proliferation; IDA:FlyBase.
DR GO; GO:0008361; P:regulation of cell size; IDA:FlyBase.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..637
FT /note="Nuclear receptor-binding protein homolog"
FT /id="PRO_0000351196"
FT DOMAIN 109..375
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 1..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 74..99
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 465..489
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 617..637
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 473
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 479
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 482
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 484
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 637 AA; 70508 MW; ABBB262CAFA44D20 CRC64;
MSNSQANAGI SGSTVADEPI QHHPSLAAGP VSASCPAATP PSQSTQQPPP HIVSASTADA
GSSAAVGVGV VAGSEGVNLD SSPRESGDDS EDESEILEES PCGRWLKRRE EVDQRDVPGI
DCVHLAMDTE EGVEVVWNEV QYASLQELKS QEEKMRQVFD NLLQLDHQNI VKFHRYWTDT
QQAERPRVVF ITEYMSSGSL KQFLKRTKRN AKRLPLESWR RWCTQILSAL SYLHSCSPPI
IHGNLTCDSI FIQHNGLVKI GSVVPDAVHY SVRRGRERER ERERGAHYFQ APEYGAADQL
TAALDIYAFG MCALEMAALE IQPSNSESTA INEETIQRTI FSLENDLQRD LIRKCLNPQP
QDRPSANDLL FHPLLFEVHS LKLLTAHCLV FSPANRTMFS ETAFDGLMQR YYQPDVVMAQ
LRLAGGQERQ YRLADVSGAD KLEKFVEDVK YGVYPLITYS GKKPPNFRSR AASPERADSV
KSATPEPVDT ESRRIVNMMC SVKIKEDSND ITMTILLRMD DKMNRQLTCQ VNENDTAADL
TSELVRLGFV HLDDQDKIQV LLEETLKAGV MSDGAGAESS GAGVTTTATM AALEQLERNW
SISSDADKQG TAVMYVPQEQ QNADGDVDVE HSGTTSN
