ID   NRDE2_CAEEL             Reviewed;        1270 AA.
AC   G5EG51;
DT   06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Nuclear exosome regulator NRDE2 {ECO:0000305};
DE   AltName: Full=Nuclear RNAi defective-2 protein;
GN   Name=nrde-2; ORFNames=T01E8.5;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2]
RP   SUBCELLULAR LOCATION, INTERACTION WITH NRDE-3, AND FUNCTION.
RX   PubMed=20543824; DOI=10.1038/nature09095;
RA   Guang S., Bochner A.F., Burkhart K.B., Burton N., Pavelec D.M., Kennedy S.;
RT   "Small regulatory RNAs inhibit RNA polymerase II during the elongation
RT   phase of transcription.";
RL   Nature 465:1097-1101(2010).
RN   [3]
RP   FUNCTION.
RX   PubMed=26365259; DOI=10.1016/j.cub.2015.07.051;
RA   Mao H., Zhu C., Zong D., Weng C., Yang X., Huang H., Liu D., Feng X.,
RA   Guang S.;
RT   "The Nrde pathway mediates small-RNA-directed histone H3 lysine 27
RT   trimethylation in Caenorhabditis elegans.";
RL   Curr. Biol. 25:2398-2403(2015).
CC   -!- FUNCTION: Protein of the nuclear speckles that regulates RNA exosomal
CC       degradation (By similarity). Involved in short interfering RNAs-
CC       mediated silencing in nuclei (PubMed:20543824). Functions with nrde-3
CC       in the nuclear RNA-mediated gene silencing (RNAi) pathway to regulate
CC       gene expression via inhibition of RNA polymerase II during the
CC       elongation phase of transcription (PubMed:20543824). Required for
CC       exogenous RNAi-induced H3K27 methylation (PubMed:26365259).
CC       {ECO:0000250|UniProtKB:Q9H7Z3, ECO:0000269|PubMed:20543824,
CC       ECO:0000269|PubMed:26365259}.
CC   -!- SUBUNIT: Interacts with nrde-3. {ECO:0000269|PubMed:20543824}.
CC   -!- INTERACTION:
CC       G5EG51; Q21691: nrde-3; NbExp=2; IntAct=EBI-16359048, EBI-2419607;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20543824}. Nucleus
CC       speckle {ECO:0000250|UniProtKB:Q9H7Z3}.
CC   -!- SIMILARITY: Belongs to the NRDE2 family. {ECO:0000305}.
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DR   EMBL; Z48809; CAA88749.1; -; Genomic_DNA.
DR   EMBL; Z48583; CAA88749.1; JOINED; Genomic_DNA.
DR   PIR; T22615; T22615.
DR   RefSeq; NP_496209.1; NM_063808.4.
DR   AlphaFoldDB; G5EG51; -.
DR   BioGRID; 39908; 3.
DR   IntAct; G5EG51; 1.
DR   STRING; 6239.T01E8.5; -.
DR   EPD; G5EG51; -.
DR   PaxDb; G5EG51; -.
DR   PeptideAtlas; G5EG51; -.
DR   PRIDE; G5EG51; -.
DR   EnsemblMetazoa; T01E8.5.1; T01E8.5.1; WBGene00011333.
DR   GeneID; 174589; -.
DR   KEGG; cel:CELE_T01E8.5; -.
DR   CTD; 174589; -.
DR   WormBase; T01E8.5; CE18165; WBGene00011333; nrde-2.
DR   eggNOG; KOG1972; Eukaryota.
DR   GeneTree; ENSGT00390000005524; -.
DR   HOGENOM; CLU_264027_0_0_1; -.
DR   InParanoid; G5EG51; -.
DR   OMA; KEIAKCR; -.
DR   OrthoDB; 1205288at2759; -.
DR   PRO; PR:G5EG51; -.
DR   Proteomes; UP000001940; Chromosome II.
DR   Bgee; WBGene00011333; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0016607; C:nuclear speck; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0031047; P:gene silencing by RNA; IMP:UniProtKB.
DR   GO; GO:0031048; P:heterochromatin assembly by small RNA; IMP:WormBase.
DR   GO; GO:1902369; P:negative regulation of RNA catabolic process; ISS:UniProtKB.
DR   GO; GO:0035194; P:post-transcriptional gene silencing by RNA; IMP:WormBase.
DR   InterPro; IPR013633; NRDE-2.
DR   PANTHER; PTHR13471; PTHR13471; 1.
DR   Pfam; PF08424; NRDE-2; 1.
PE   1: Evidence at protein level;
KW   Nucleus; Reference proteome; RNA-mediated gene silencing.
FT   CHAIN           1..1270
FT                   /note="Nuclear exosome regulator NRDE2"
FT                   /id="PRO_0000420972"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          119..209
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        119..150
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        151..165
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        166..187
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        188..203
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1270 AA;  147636 MW;  DCB661227D75F541 CRC64;
     MFRAYGNNGL KNPERISGEN PDLYTQTRAA VQQRATTTLK RNEKQKLAVQ NDSVFQQVGI
     GESDSDDDNG GVRIRMSPHR YIDPDDVFTL PEVKKQNALR DAKIAARAAQ ATAYNTFPSV
     KSLNGCQDPP ETSQQSTSRK RSASNSRSPS RSHSRRYDRD NGRQRSRSRE KKRRKKERRR
     KRSSSRSSSS SRSRDRSSRA RDTSSHTLMK MNKPAKYAFL TDEEYRTCDA YISSAFITQT
     KSDCENYTQG VPKKEIAKCR LSVKFIVGLE HNNILFNNIY GAEYARDKEN RPFWEQLDRY
     LKDVPKETFF RYVPPVGGYW KIRDRVDLLN LDHIDDDVLA NDSDNRKDAF TFELEQAKKT
     FSENVHNIDA LIKVISMEEE MCRRNVGSFS SSNPAALAER HQEMVKKAIK ADGRNAKLRL
     MKIELLIKMD PNSPTIIDDF KNLTITFPHE PMVWIKYLDY IQYDSNVYNY KKLKNAFEDC
     IRQVTGLTNG TLLSHLNAVN DRPLLRMFHL WIYIRYLKWM ISCAHTPVVL ANIQATFEYN
     FGLADVEKRT STNSKEREVR LEEFWESGLP RIGDEGAVGA EKMLKQSEEL SDEDIQKLEN
     DDFDILISRT EETIATCLQA QRDVQISWIE VEREMMNIDA RVKRTKLKDC ELYEDHVDDL
     ETCELWDIIP FDRIRYYEAP GDCANFDFVQ PFLELLGVKF LNSTNCFTTT EQIISDWISN
     DSTVNFYKTP TYTEKKCFEV GNNILKFMLY NRLKLTENNP EYLDKTMVKY LLAMLVTEAS
     EQEKKLNFHS FKLNLKNLVG TFITKHPDIF KRAMLSKITG IVYMEKFVSW WERALKEQEK
     VVEADERRKN YKEIKMEEGV VDDVKFDVIL LKKDKERVQT IRDKIRDMID IAIPKSTEKL
     IQSADSSLPT LQLHLYANVL RGRLSILNQN ALEETRDVFC KEILGIHTSE FESDEALLLA
     LDQGLNELLE HCKEKDNLES VDSIPELPRA EALCEALKVV AVFVFLDKMA FSRRAVDCLI
     ANAITKFEQF EAKKNDFNRG TYEKYCDQID LKFITDTLIT FFSHKKHRFI YNENFKKLIF
     QASQAFPCDS KYAKMLGELH SSGRLQVMKL QGFTDSRNSI LNAKRDQQFD PELETRLLMN
     SLTIMFSWMN AANRIGDAGN QILYKNWKRE AANTRDPAIW RQVIRVASKL SQKILKDDAY
     TRARGQCTWA LNLHFDYIEA KTVRKNGDLM EMIYLILEQS MGQEHSLFVT DEEYMKTQQE
     IGLQYSESGR