NRDE3_CAEEL
ID NRDE3_CAEEL Reviewed; 1057 AA.
AC Q21691;
DT 06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Nuclear RNAi defective-3 protein;
GN Name=nrde-3; ORFNames=R04A9.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF LYS-80; ARG-81; LYS-82;
RP TYR-463 AND TYR-464.
RX PubMed=18653886; DOI=10.1126/science.1157647;
RA Guang S., Bochner A.F., Pavelec D.M., Burkhart K.B., Harding S.,
RA Lachowiec J., Kennedy S.;
RT "An Argonaute transports siRNAs from the cytoplasm to the nucleus.";
RL Science 321:537-541(2008).
RN [3]
RP INTERACTION WITH NRDE-2.
RX PubMed=20543824; DOI=10.1038/nature09095;
RA Guang S., Bochner A.F., Burkhart K.B., Burton N., Pavelec D.M., Kennedy S.;
RT "Small regulatory RNAs inhibit RNA polymerase II during the elongation
RT phase of transcription.";
RL Nature 465:1097-1101(2010).
RN [4]
RP FUNCTION.
RX PubMed=26365259; DOI=10.1016/j.cub.2015.07.051;
RA Mao H., Zhu C., Zong D., Weng C., Yang X., Huang H., Liu D., Feng X.,
RA Guang S.;
RT "The Nrde pathway mediates small-RNA-directed histone H3 lysine 27
RT trimethylation in Caenorhabditis elegans.";
RL Curr. Biol. 25:2398-2403(2015).
CC -!- FUNCTION: Transports small interfering RNAs (siRNAs) from the cytoplasm
CC to the nucleus. Required for RNA interference (RNAi) in nuclei
CC (PubMed:18653886). Required for exogenous RNAi-induced H3K27
CC methylation (PubMed:26365259). {ECO:0000269|PubMed:18653886,
CC ECO:0000269|PubMed:26365259}.
CC -!- INTERACTION:
CC Q21691; G5EG51: nrde-2; NbExp=2; IntAct=EBI-2419607, EBI-16359048;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18653886}. Nucleus
CC {ECO:0000269|PubMed:18653886}. Note=siRNA binding is necessary and
CC sufficient for distribution of nrde-3 to the nucleus.
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DR EMBL; FO080331; CCD62910.1; -; Genomic_DNA.
DR PIR; T16676; T16676.
DR RefSeq; NP_508092.1; NM_075691.4.
DR AlphaFoldDB; Q21691; -.
DR SMR; Q21691; -.
DR BioGRID; 45345; 3.
DR DIP; DIP-55715N; -.
DR IntAct; Q21691; 2.
DR STRING; 6239.R04A9.2; -.
DR EPD; Q21691; -.
DR PaxDb; Q21691; -.
DR PeptideAtlas; Q21691; -.
DR PRIDE; Q21691; -.
DR EnsemblMetazoa; R04A9.2.1; R04A9.2.1; WBGene00019862.
DR GeneID; 180392; -.
DR KEGG; cel:CELE_R04A9.2; -.
DR UCSC; R04A9.2; c. elegans.
DR CTD; 180392; -.
DR WormBase; R04A9.2; CE04789; WBGene00019862; nrde-3.
DR eggNOG; KOG1041; Eukaryota.
DR HOGENOM; CLU_012806_0_0_1; -.
DR InParanoid; Q21691; -.
DR OMA; YTRGPNA; -.
DR OrthoDB; 540725at2759; -.
DR PhylomeDB; Q21691; -.
DR Reactome; R-CEL-203927; MicroRNA (miRNA) biogenesis.
DR Reactome; R-CEL-426486; Small interfering RNA (siRNA) biogenesis.
DR Reactome; R-CEL-5578749; Transcriptional regulation by small RNAs.
DR PRO; PR:Q21691; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00019862; Expressed in embryo and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0031332; C:RNAi effector complex; IDA:WormBase.
DR GO; GO:0004521; F:endoribonuclease activity; IBA:GO_Central.
DR GO; GO:0035198; F:miRNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003727; F:single-stranded RNA binding; IBA:GO_Central.
DR GO; GO:0035197; F:siRNA binding; IDA:WormBase.
DR GO; GO:0008355; P:olfactory learning; IMP:WormBase.
DR GO; GO:0035194; P:post-transcriptional gene silencing by RNA; IMP:WormBase.
DR GO; GO:1900368; P:regulation of post-transcriptional gene silencing by RNA; IMP:CACAO.
DR GO; GO:0006404; P:RNA import into nucleus; IMP:WormBase.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR003100; PAZ_dom.
DR InterPro; IPR036085; PAZ_dom_sf.
DR InterPro; IPR003165; Piwi.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF02170; PAZ; 1.
DR Pfam; PF02171; Piwi; 1.
DR SMART; SM00949; PAZ; 1.
DR SMART; SM00950; Piwi; 1.
DR SUPFAM; SSF101690; SSF101690; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR PROSITE; PS50821; PAZ; 1.
DR PROSITE; PS50822; PIWI; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome; RNA-mediated gene silencing.
FT CHAIN 1..1057
FT /note="Nuclear RNAi defective-3 protein"
FT /id="PRO_0000420973"
FT DOMAIN 385..500
FT /note="PAZ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00142"
FT DOMAIN 677..1001
FT /note="Piwi"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00150"
FT REGION 1..89
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 344..388
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 18..61
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 66..80
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 354..388
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 80
FT /note="K->A: No nuclear localization."
FT /evidence="ECO:0000269|PubMed:18653886"
FT MUTAGEN 81
FT /note="R->A: No nuclear localization."
FT /evidence="ECO:0000269|PubMed:18653886"
FT MUTAGEN 82
FT /note="K->A: No nuclear localization."
FT /evidence="ECO:0000269|PubMed:18653886"
FT MUTAGEN 463
FT /note="Y->A: Fails to interact with siRNAs and localizes
FT predominantly to the cytoplasm."
FT /evidence="ECO:0000269|PubMed:18653886"
FT MUTAGEN 464
FT /note="Y->A: Fails to interact with siRNAs and localizes
FT predominantly to the cytoplasm."
FT /evidence="ECO:0000269|PubMed:18653886"
SQ SEQUENCE 1057 AA; 119713 MW; C86F4A37DE64F519 CRC64;
MDLLDKVMGE MGSKPGSTAK KPATSASSTP RTNVWGTAKK PSSQQQPPKP LFTTPGSQQG
SLGGRIPKRE HTDRTGPDPK RKPLGGLSVP DSFNNFGTFR VQMNAWNLDI SKMDERISRI
MFRATLVHTD GRRFELSLGV SAFSGDVNRQ QRRQAQCLLF RAWFKRNPEL FKGMTDPAIA
AYDAAETIYV GCSFFDVELT EHVCHLTEAD FSPQEWKIVS LISRRSGSTF EIRIKTNPPI
YTRGPNALTL ENRSELTRII EAITDQCLHN EKFLLYSSGT FPTKGGDIAS PDEVTLIKSG
FVKTTKIVDR DGVPDAIMTV DTTKSPFYKD TSLLKFFTAK MDQLTNSGGG PRGHNGGRER
RDGGGNSRKY DDRRSPRDGE IDYDERTVSH YQRQFQDERI SDGMLNTLKQ SLKGLDCQPI
HLKDSKANRS IMIDEIHTGT ADSVTFEQKL PDGEMKLTSI TEYYLQRYNY RLKFPHLPLV
TSKRAKCYDF YPMELMSILP GQRIKQSHMT VDIQSYMTGK MSSLPDQHIK QSKLVLTEYL
KLGDQPANRQ MDAFRVSLKS IQPIVTNAHW LSPPDMKFAN NQLYSLNPTR GVRFQTNGKF
VMPARVKSVT IINYDKEFNR NVDMFAEGLA KHCSEQGMKF DSRPNSWKKV NLGSSDRRGT
KVEIEEAIRN GVTIVFGIIA EKRPDMHDIL KYFEEKLGQQ TIQISSETAD KFMRDHGGKQ
TIDNVIRKLN PKCGGTNFLI DVPESVGHRV VCNNSAEMRA KLYAKTQFIG FEMSHTGART
RFDIQKVMFD GDPTVVGVAY SLKHSAQLGG FSYFQESRLH KLTNLQEKMQ ICLNAYEQSS
SYLPETVVVY RVGSGEGDYP QIVNEVNEMK LAARKKKHGY NPKFLVICTQ RNSHIRVFPE
HINERGKSME QNVKSGTCVD VPGASHGYEE FILCCQTPLI GTVKPTKYTI IVNDCRWSKN
EIMNVTYHLA FAHQVSYAPP AIPNVSYAAQ NLAKRGHNNY KTHTKLVDMN DYSYRIKEKH
EEIISSEEVD DILMRDFIET VSNDLNAMTI NGRNFWA
