NRDI_ECOLI
ID NRDI_ECOLI Reviewed; 136 AA.
AC P0A772; P77025; Q47415;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Protein NrdI;
GN Name=nrdI; Synonyms=ygaO; OrderedLocusNames=b2674, JW2649;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8820648; DOI=10.1046/j.1365-2958.1996.424950.x;
RA Jordan A., Aragall E., Gibert I., Barbe J.;
RT "Promoter identification and expression analysis of Salmonella typhimurium
RT and Escherichia coli nrdEF operons encoding one of two class I
RT ribonucleotide reductases present in both bacteria.";
RL Mol. Microbiol. 19:777-790(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP INDUCTION BY HYDROXYUREA.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=20005847; DOI=10.1016/j.molcel.2009.11.024;
RA Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J.,
RA Walker G.C.;
RT "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia
RT coli.";
RL Mol. Cell 36:845-860(2009).
CC -!- FUNCTION: Probably involved in ribonucleotide reductase function.
CC {ECO:0000250}.
CC -!- INDUCTION: Induced 2-fold by hydroxyurea.
CC {ECO:0000269|PubMed:20005847}.
CC -!- SIMILARITY: Belongs to the NrdI family. {ECO:0000305}.
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DR EMBL; X79787; CAA56185.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75721.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16538.2; -; Genomic_DNA.
DR PIR; S70890; S70890.
DR RefSeq; NP_417160.1; NC_000913.3.
DR RefSeq; WP_000080947.1; NZ_STEB01000042.1.
DR PDB; 3N39; X-ray; 2.50 A; C/D=1-133.
DR PDB; 3N3A; X-ray; 1.99 A; C/D=1-133.
DR PDB; 3N3B; X-ray; 2.36 A; C/D=1-133.
DR PDBsum; 3N39; -.
DR PDBsum; 3N3A; -.
DR PDBsum; 3N3B; -.
DR AlphaFoldDB; P0A772; -.
DR SMR; P0A772; -.
DR BioGRID; 4262268; 9.
DR IntAct; P0A772; 27.
DR STRING; 511145.b2674; -.
DR PaxDb; P0A772; -.
DR PRIDE; P0A772; -.
DR EnsemblBacteria; AAC75721; AAC75721; b2674.
DR EnsemblBacteria; BAA16538; BAA16538; BAA16538.
DR GeneID; 60903588; -.
DR GeneID; 67414032; -.
DR GeneID; 947158; -.
DR KEGG; ecj:JW2649; -.
DR KEGG; eco:b2674; -.
DR PATRIC; fig|1411691.4.peg.4067; -.
DR EchoBASE; EB3072; -.
DR eggNOG; COG1780; Bacteria.
DR HOGENOM; CLU_114845_0_0_6; -.
DR InParanoid; P0A772; -.
DR OMA; NTHRFVG; -.
DR PhylomeDB; P0A772; -.
DR BioCyc; EcoCyc:G7402-MON; -.
DR EvolutionaryTrace; P0A772; -.
DR PRO; PR:P0A772; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0010181; F:FMN binding; IDA:EcoCyc.
DR GO; GO:0036211; P:protein modification process; IBA:GO_Central.
DR Gene3D; 3.40.50.360; -; 1.
DR HAMAP; MF_00128; NrdI; 1.
DR InterPro; IPR029039; Flavoprotein-like_sf.
DR InterPro; IPR020852; RNR_Ib_NrdI_bac.
DR InterPro; IPR004465; RNR_NrdI.
DR PANTHER; PTHR37297; PTHR37297; 1.
DR Pfam; PF07972; Flavodoxin_NdrI; 1.
DR PIRSF; PIRSF005087; NrdI; 1.
DR SUPFAM; SSF52218; SSF52218; 1.
DR TIGRFAMs; TIGR00333; nrdI; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome.
FT CHAIN 1..136
FT /note="Protein NrdI"
FT /id="PRO_0000164314"
FT STRAND 4..6
FT /evidence="ECO:0007829|PDB:3N3A"
FT HELIX 13..21
FT /evidence="ECO:0007829|PDB:3N3A"
FT STRAND 25..27
FT /evidence="ECO:0007829|PDB:3N3B"
FT STRAND 30..33
FT /evidence="ECO:0007829|PDB:3N39"
FT STRAND 42..47
FT /evidence="ECO:0007829|PDB:3N3A"
FT STRAND 52..58
FT /evidence="ECO:0007829|PDB:3N3A"
FT HELIX 60..66
FT /evidence="ECO:0007829|PDB:3N3A"
FT HELIX 69..74
FT /evidence="ECO:0007829|PDB:3N3A"
FT STRAND 75..82
FT /evidence="ECO:0007829|PDB:3N3A"
FT HELIX 84..89
FT /evidence="ECO:0007829|PDB:3N3A"
FT HELIX 92..101
FT /evidence="ECO:0007829|PDB:3N3A"
FT STRAND 105..110
FT /evidence="ECO:0007829|PDB:3N3A"
FT HELIX 115..130
FT /evidence="ECO:0007829|PDB:3N3A"
SQ SEQUENCE 136 AA; 15340 MW; 2834D808D18EBCBB CRC64;
MSQLVYFSSS SENTQRFIER LGLPAVRIPL NERERIQVDE PYILIVPSYG GGGTAGAVPR
QVIRFLNDEH NRALLRGVIA SGNRNFGEAY GRAGDVIARK CGVPWLYRFE LMGTQSDIEN
VRKGVTEFWQ RQPQNA
