ID   NREA_HALVD              Reviewed;         420 AA.
AC   D4GTA5;
DT   20-DEC-2017, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=DNA repair protein NreA {ECO:0000305};
DE   AltName: Full=PCNA interacting protein NreA {ECO:0000303|PubMed:26337406};
GN   Name=nreA {ECO:0000303|PubMed:26337406};
GN   OrderedLocusNames=HVO_0734 {ECO:0000312|EMBL:ADE03741.1};
OS   Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS   NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC   Haloferacaceae; Haloferax.
OX   NCBI_TaxID=309800;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC   B-1768 / DS2;
RX   PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA   Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA   Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA   Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT   "The complete genome sequence of Haloferax volcanii DS2, a model
RT   archaeon.";
RL   PLoS ONE 5:E9605-E9605(2010).
RN   [2]
RP   FUNCTION, NOMENCLATURE, DISRUPTION PHENOTYPE, PIP MOTIF, AND MUTAGENESIS OF
RP   411-GLY--ALA-420.
RC   STRAIN=DS2 / DS70;
RX   PubMed=26337406; DOI=10.1111/mmi.13210;
RA   Giroux X., MacNeill S.A.;
RT   "A novel archaeal DNA repair factor that acts with the UvrABC system to
RT   repair mitomycin C-induced DNA damage in a PCNA-dependent manner.";
RL   Mol. Microbiol. 99:1-14(2016).
CC   -!- FUNCTION: Involved in DNA damage repair. Works together with the UvrABC
CC       proteins in repairing DNA damage resulting from exposure to the DNA
CC       damaging agent mitomycin C (MMC). {ECO:0000269|PubMed:26337406}.
CC   -!- SUBUNIT: Interacts with the DNA polymerase sliding clamp (PCNA) via the
CC       PIP (PCNA-interacting peptide) motif. {ECO:0000305|PubMed:26337406}.
CC   -!- DISRUPTION PHENOTYPE: Non-essential, but cells lacking this gene are
CC       sensitive to DNA damage induced by treatment with MMC. Does not display
CC       increased sensitivity to UV light or to the alkylating agent methyl
CC       methanesulphonate (MMS). {ECO:0000269|PubMed:26337406}.
CC   -!- SIMILARITY: Belongs to the Nre family. {ECO:0000255|HAMAP-
CC       Rule:MF_02096, ECO:0000305|PubMed:26337406}.
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DR   EMBL; CP001956; ADE03741.1; -; Genomic_DNA.
DR   RefSeq; WP_004044203.1; NZ_AOHU01000097.1.
DR   AlphaFoldDB; D4GTA5; -.
DR   STRING; 309800.C498_15063; -.
DR   EnsemblBacteria; ADE03741; ADE03741; HVO_0734.
DR   GeneID; 8926669; -.
DR   KEGG; hvo:HVO_0734; -.
DR   PATRIC; fig|309800.29.peg.2907; -.
DR   eggNOG; arCOG04269; Archaea.
DR   HOGENOM; CLU_039703_0_0_2; -.
DR   OMA; PVGVWQI; -.
DR   OrthoDB; 17407at2157; -.
DR   Proteomes; UP000008243; Chromosome.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_02096; Nre; 1.
DR   InterPro; IPR033167; Nre.
DR   InterPro; IPR006979; Nre_C.
DR   InterPro; IPR006978; Nre_N.
DR   PANTHER; PTHR38136; PTHR38136; 1.
DR   Pfam; PF04895; Nre_C; 1.
DR   Pfam; PF04894; Nre_N; 1.
PE   1: Evidence at protein level;
KW   DNA damage; DNA repair; Reference proteome.
FT   CHAIN           1..420
FT                   /note="DNA repair protein NreA"
FT                   /id="PRO_0000442518"
FT   MOTIF           413..420
FT                   /note="PIP motif"
FT                   /evidence="ECO:0000305|PubMed:26337406"
FT   MUTAGEN         411..420
FT                   /note="Missing: Displays increased chromosome fragmentation
FT                   and delayed recovery relative to wild-type cells."
FT                   /evidence="ECO:0000269|PubMed:26337406"
SQ   SEQUENCE   420 AA;  46864 MW;  4B84C16C6A9E6A7F CRC64;
     MRLDEYMDIE RDERAERRRL AEEKSYGILD HLETFQDRFE ETVQGDSLYG GVSPSIFVGR
     SNYPNVSTGI LSPVGHDEDA ASFETSAAWY DEGVSIDDVF QRRTSLLNSN RGTKVTNVAD
     SWDGFLGTQR EVAIADRPVT VEIGLDGKPS LDLDASADDV ATPVGPRARA RSADLAENPH
     VPKLVKKTLE DDDWNAEGAM TYLYRRGFDV YDINTILSAG ALGQTEQRRL VPTRWSITAV
     DDTLGQYLRG QVKHAESVDG VEIYRNEFIG NAFWVILAPG RWEFELIELK APGSVWNPDP
     EAGMYLAADR EGYEGRTGYV NETAGAYHAS RLGVLEHLQE RGRQAKALVI RHVSDDYWGP
     VGVWQIRESI RHAFEGEMAD AETFGDAVRD VTEYLPVSLA DLRRKSTMAA GLQTDIFDFA