ID   NREC_STACT              Reviewed;         217 AA.
AC   Q7WZY4; B9DL89; Q9ZB65;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Oxygen regulatory protein NreC;
DE   AltName: Full=Nitrogen regulation protein C;
GN   Name=nreC; OrderedLocusNames=Sca_1888;
OS   Staphylococcus carnosus (strain TM300).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=396513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A TRANSCRIPTIONAL REGULATOR,
RP   AND PHOSPHORYLATION.
RX   PubMed=12426351; DOI=10.1128/jb.184.23.6624-6634.2002;
RA   Fedtke I., Kamps A., Krismer B., Goetz F.;
RT   "The nitrate reductase and nitrite reductase operons and the narT gene of
RT   Staphylococcus carnosus are positively controlled by the novel two-
RT   component system NreBC.";
RL   J. Bacteriol. 184:6624-6634(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TM300;
RX   PubMed=19060169; DOI=10.1128/aem.01982-08;
RA   Rosenstein R., Nerz C., Biswas L., Resch A., Raddatz G., Schuster S.C.,
RA   Goetz F.;
RT   "Genome analysis of the meat starter culture bacterium Staphylococcus
RT   carnosus TM300.";
RL   Appl. Environ. Microbiol. 75:811-822(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 33-217.
RA   Fast B., Goetz F.;
RL   Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Member of the two-component regulatory system NreB/NreC
CC       involved in the control of dissimilatory nitrate/nitrite reduction in
CC       response to oxygen. Phosphorylated NreC binds to a GC-rich palindromic
CC       sequence at the promoters of the nitrate (narGHJI) and nitrite (nir)
CC       reductase operons, as well as the putative nitrate transporter gene
CC       narT, and activates their expression. {ECO:0000269|PubMed:12426351}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by NreB. {ECO:0000269|PubMed:12426351}.
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DR   EMBL; AF029224; AAP79640.1; -; Genomic_DNA.
DR   EMBL; AM295250; CAL28794.1; -; Genomic_DNA.
DR   EMBL; U40158; AAD10462.1; -; Genomic_DNA.
DR   RefSeq; WP_015901130.1; NC_012121.1.
DR   AlphaFoldDB; Q7WZY4; -.
DR   SMR; Q7WZY4; -.
DR   STRING; 396513.SCA_1888; -.
DR   GeneID; 60544398; -.
DR   KEGG; sca:SCA_1888; -.
DR   eggNOG; COG2197; Bacteria.
DR   HOGENOM; CLU_000445_90_1_9; -.
DR   OMA; LDKYATH; -.
DR   OrthoDB; 1687028at2; -.
DR   BioCyc; SCAR396513:SCA_RS09580-MON; -.
DR   Proteomes; UP000000444; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd06170; LuxR_C_like; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   Pfam; PF00196; GerE; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   PRINTS; PR00038; HTHLUXR.
DR   SMART; SM00421; HTH_LUXR; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS00622; HTH_LUXR_1; 1.
DR   PROSITE; PS50043; HTH_LUXR_2; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   1: Evidence at protein level;
KW   Activator; Cytoplasm; DNA-binding; Phosphoprotein; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..217
FT                   /note="Oxygen regulatory protein NreC"
FT                   /id="PRO_0000349356"
FT   DOMAIN          2..119
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DOMAIN          148..213
FT                   /note="HTH luxR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   DNA_BIND        172..191
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   MOD_RES         53
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   217 AA;  24467 MW;  8914A95426D7656B CRC64;
     MKIVIADDHA VVRTGFSMIL NFQDDMEVVD TAADGVEAYQ KVMQHQPDVL IMDLSMPPGE
     SGLIATSKIV ESFPDTKILI LTMYDDEEYL FHVLRNGAKG YILKNAPDEQ LISAVRTVYR
     GDTYIDPKMT TSLVNEFVNN TGQDANSTND PFRILSKREL EILPLIAKGY GNKEIAEKLF
     VSVKTVEAHK THIMQKLNLK SKPELVEYAL KKKLLDF