NREP_MOUSE
ID NREP_MOUSE Reviewed; 68 AA.
AC Q07475;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Neuronal regeneration-related protein;
DE AltName: Full=Neuronal protein 3.1;
DE AltName: Full=Protein p311;
GN Name=Nrep; Synonyms=D0H4S114, P311;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Brain;
RX PubMed=8261136; DOI=10.1111/j.1460-9568.1993.tb00527.x;
RA Studler J.-M., Glowinski J., Levi-Strauss M.;
RT "An abundant mRNA of the embryonic brain persists at a high level in
RT cerebellum, hippocampus and olfactory bulb during adulthood.";
RL Eur. J. Neurosci. 5:614-623(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J; TISSUE=Urinary bladder;
RA Adachi J., Aizawa K., Akahira S., Akimura T., Aono H., Arai A., Arakawa T.,
RA Carninci P., Fukuda S., Fukunishi Y., Furuno M., Hanagaki T., Hara A.,
RA Hayatsu N., Hiramoto K., Hiraoka T., Hori F., Imotani K., Ishii Y.,
RA Itoh M., Izawa M., Kato H., Kawai J., Kojima Y., Konno H., Kouda M.,
RA Koya S., Kurihara C., Matsuyama T., Miyazaki A., Nishi K., Nomura K.,
RA Numazaki R., Ohno M., Okazaki Y., Okido T., Owa C., Saito H., Saito R.,
RA Sakai C., Sakai K., Sano H., Sasaki D., Shibata K., Shibata Y.,
RA Shinagawa A., Shiraki T., Sogabe Y., Suzuki H., Tagami M., Tagawa A.,
RA Takahashi F., Tanaka T., Tejima Y., Toya T., Yamamura T., Yasunishi A.,
RA Yoshida K., Yoshino M., Muramatsu M., Hayashizaki Y.;
RL Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION.
RX PubMed=12417574; DOI=10.1172/jci15614;
RA Pan D., Zhe X., Jakkaraju S., Taylor G.A., Schuger L.;
RT "P311 induces a TGF-beta1-independent, nonfibrogenic myofibroblast
RT phenotype.";
RL J. Clin. Invest. 110:1349-1358(2002).
RN [5]
RP FUNCTION, AND INTERACTION WITH TGFB1 AND TGFB2.
RX PubMed=14985127; DOI=10.1016/j.bbrc.2004.01.171;
RA Paliwal S., Shi J., Dhru U., Zhou Y., Schuger L.;
RT "P311 binds to the latency associated protein and downregulates the
RT expression of TGF-beta1 and TGF-beta2.";
RL Biochem. Biophys. Res. Commun. 315:1104-1109(2004).
RN [6]
RP POSSIBLE FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=16484684; DOI=10.1165/rcmb.2005-0475oc;
RA Zhao L., Leung J.K., Yamamoto H., Goswami S., Kheradmand F., Vu T.H.;
RT "Identification of P311 as a potential gene regulating alveolar
RT generation.";
RL Am. J. Respir. Cell Mol. Biol. 35:48-54(2006).
RN [7]
RP FUNCTION.
RX PubMed=16934802; DOI=10.1016/j.yexcr.2006.07.016;
RA Shi J., Badri K.R., Choudhury R., Schuger L.;
RT "P311-induced myofibroblasts exhibit ameboid-like migration through RalA
RT activation.";
RL Exp. Cell Res. 312:3432-3442(2006).
RN [8]
RP DISRUPTION PHENOTYPE.
RX PubMed=18616608; DOI=10.1111/j.1601-183x.2008.00420.x;
RA Taylor G.A., Rodriguiz R.M., Greene R.I., Daniell X., Henry S.C.,
RA Crooks K.R., Kotloski R., Tessarollo L., Phillips L.E., Wetsel W.C.;
RT "Behavioral characterization of P311 knockout mice.";
RL Genes Brain Behav. 7:786-795(2008).
RN [9]
RP FUNCTION.
RX PubMed=18664493; DOI=10.1242/jcs.027151;
RA Leung J.K., Cases S., Vu T.H.;
RT "P311 functions in an alternative pathway of lipid accumulation that is
RT induced by retinoic acid.";
RL J. Cell Sci. 121:2751-2758(2008).
RN [10]
RP DISRUPTION PHENOTYPE.
RX PubMed=18549486; DOI=10.1186/1744-8069-4-23;
RA Sun Y.-G., Gao Y.-J., Zhao Z.-Q., Huang B., Yin J., Taylor G.A.,
RA Chen Z.-F.;
RT "Involvement of P311 in the affective, but not in the sensory component of
RT pain.";
RL Mol. Pain 4:23-23(2008).
CC -!- FUNCTION: May have roles in cellular differentiation. Ectopic
CC expression induces differentiation of fibroblast into myofibroblast and
CC myofibroblast ameboid migration. Increases retinoic-acid regulation of
CC lipid-droplet biogenesis. May also have neural functions. Promotes
CC axonal regeneration and augments motility of gliomas. Down-regulates
CC the expression of TGFB1 and TGFB2 but not of TGFB3. May play a role in
CC the regulation of alveolar generation. {ECO:0000269|PubMed:12417574,
CC ECO:0000269|PubMed:14985127, ECO:0000269|PubMed:16934802,
CC ECO:0000269|PubMed:18664493}.
CC -!- SUBUNIT: Interacts with FLNA (By similarity). Interacts with the
CC latency-associated peptides (LAP) of TGFB1 and TGFB2; the interaction
CC results in a decrease in TGFB autoinduction. {ECO:0000250,
CC ECO:0000269|PubMed:14985127}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in brain and fetal lung.
CC {ECO:0000269|PubMed:16484684, ECO:0000269|PubMed:8261136}.
CC -!- DEVELOPMENTAL STAGE: Expressed mainly in neurons belonging to a late
CC migration wave. During adulthood, it persists at a high level in the
CC granular layer of the cerebellum, the hippocampus and the olfactory
CC bulb. In lung, detected at low levels at the pseudoglandular stage
CC (14.5 dpc), with expression starting to increase at the beginning of
CC distal lung development (16.5 dpc) and remaining at the same level
CC during saccular development (18.5 dpc). The expression levels are
CC highest at the beginning of and during alveolar development (P5-P14)
CC before decreasing again to low levels at the end of alveolization
CC (P30). {ECO:0000269|PubMed:16484684, ECO:0000269|PubMed:8261136}.
CC -!- INDUCTION: Down-regulated in lungs by dexamethasone treatment.
CC {ECO:0000269|PubMed:16484684}.
CC -!- PTM: Phosphorylated on Ser-59. Phosphorylation decreases stability and
CC activity. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Normal heat and mechanical sensitivity, as well
CC as normal formalin-induced inflammatory pain. Reduced formalin-induced
CC avoidance behavior, which reflects pain-related negative emotion,
CC indicating a role in the affective, but not in the sensory component of
CC the pain. Also exhibits altered behavioral responses in learning and
CC memory. {ECO:0000269|PubMed:18549486, ECO:0000269|PubMed:18616608}.
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DR EMBL; X70398; CAA49848.1; -; mRNA.
DR EMBL; AK020629; BAB32157.1; -; mRNA.
DR EMBL; BC054762; AAH54762.1; -; mRNA.
DR CCDS; CCDS37756.1; -.
DR PIR; S29993; S29993.
DR RefSeq; NP_001103458.1; NM_001109988.1.
DR RefSeq; NP_001103459.1; NM_001109989.2.
DR RefSeq; NP_001254646.1; NM_001267717.1.
DR RefSeq; NP_444308.1; NM_053078.4.
DR AlphaFoldDB; Q07475; -.
DR BioGRID; 205307; 2.
DR STRING; 10090.ENSMUSP00000058132; -.
DR PhosphoSitePlus; Q07475; -.
DR PaxDb; Q07475; -.
DR PRIDE; Q07475; -.
DR Antibodypedia; 632; 124 antibodies from 24 providers.
DR DNASU; 27528; -.
DR Ensembl; ENSMUST00000051087; ENSMUSP00000058132; ENSMUSG00000042834.
DR Ensembl; ENSMUST00000168890; ENSMUSP00000130297; ENSMUSG00000042834.
DR Ensembl; ENSMUST00000171533; ENSMUSP00000127787; ENSMUSG00000042834.
DR Ensembl; ENSMUST00000237066; ENSMUSP00000157912; ENSMUSG00000042834.
DR GeneID; 27528; -.
DR KEGG; mmu:27528; -.
DR UCSC; uc008ejt.2; mouse.
DR CTD; 9315; -.
DR MGI; MGI:99444; Nrep.
DR VEuPathDB; HostDB:ENSMUSG00000042834; -.
DR eggNOG; ENOG502SFKT; Eukaryota.
DR GeneTree; ENSGT00390000016521; -.
DR HOGENOM; CLU_204758_0_0_1; -.
DR InParanoid; Q07475; -.
DR OMA; IPPGNHE; -.
DR OrthoDB; 1603548at2759; -.
DR PhylomeDB; Q07475; -.
DR TreeFam; TF336368; -.
DR BioGRID-ORCS; 27528; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Nrep; mouse.
DR PRO; PR:Q07475; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q07475; protein.
DR Bgee; ENSMUSG00000042834; Expressed in external carotid artery and 260 other tissues.
DR ExpressionAtlas; Q07475; baseline and differential.
DR Genevisible; Q07475; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0031103; P:axon regeneration; IBA:GO_Central.
DR GO; GO:0045664; P:regulation of neuron differentiation; ISO:MGI.
DR GO; GO:0017015; P:regulation of transforming growth factor beta receptor signaling pathway; IDA:MGI.
DR InterPro; IPR024417; Neuronal_3.1.
DR PANTHER; PTHR17102; PTHR17102; 1.
DR Pfam; PF11092; Alveol-reg_P311; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..68
FT /note="Neuronal regeneration-related protein"
FT /id="PRO_0000057938"
FT REGION 42..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 68 AA; 7688 MW; FAC111CFE0AEFEAF CRC64;
MVYYPELLVW VSQEPFAYKE MEGGLIKGRL PVPKEVNRKK MEETGAASLT PPGSREFTSP
ATSYLHPF
