ID   NRFA_STAA8              Reviewed;         251 AA.
AC   Q2G0Z5;
DT   22-AUG-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=NADPH-dependent oxidoreductase;
DE            EC=1.6.-.-;
GN   Name=nfrA; OrderedLocusNames=SAOUHSC_00366;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=15774866; DOI=10.1128/jb.187.7.2249-2256.2005;
RA   Streker K., Freiberg C., Labischinski H., Hacker J., Ohlsen K.;
RT   "Staphylococcus aureus NfrA (SA0367) is a flavin mononucleotide-dependent
RT   NADPH oxidase involved in oxidative stress response.";
RL   J. Bacteriol. 187:2249-2256(2005).
CC   -!- FUNCTION: Reduces FMN, organic nitro compounds and disulfide DTNB.
CC       Involved in maintenance of the cellular redox state and the disulfide
CC       stress response.
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=17 uM for NADPH;
CC         Vmax=16 umol/min/mg enzyme toward FMN (at 21 degrees Celsius and at
CC         pH 6.8 in the presence of 50 mM NaCl);
CC         Vmax=20 umol/min/mg enzyme toward nitrofurazone (at 21 degrees
CC         Celsius and at pH 6.8 in the presence of 50 mM NaCl);
CC         Vmax=15 umol/min/mg enzyme toward nitrofurantoin (at 21 degrees
CC         Celsius and at pH 6.8 in the presence of 50 mM NaCl);
CC         Vmax=97 umol/min/mg enzyme toward ferricyanide (at 21 degrees Celsius
CC         and at pH 6.8 in the presence of 50 mM NaCl);
CC         Vmax=0.12 umol/min/mg enzyme toward DTNB (at 21 degrees Celsius and
CC         at pH 6.8 in the presence of 50 mM NaCl);
CC   -!- INDUCTION: By ethanol stress and disulfide stress.
CC   -!- SIMILARITY: Belongs to the flavin oxidoreductase frp family.
CC       {ECO:0000305}.
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DR   EMBL; CP000253; ABD29532.1; -; Genomic_DNA.
DR   RefSeq; WP_001289311.1; NZ_LS483365.1.
DR   RefSeq; YP_498955.1; NC_007795.1.
DR   AlphaFoldDB; Q2G0Z5; -.
DR   SMR; Q2G0Z5; -.
DR   STRING; 1280.SAXN108_0432; -.
DR   EnsemblBacteria; ABD29532; ABD29532; SAOUHSC_00366.
DR   GeneID; 3919785; -.
DR   KEGG; sao:SAOUHSC_00366; -.
DR   PATRIC; fig|93061.5.peg.336; -.
DR   eggNOG; COG0778; Bacteria.
DR   HOGENOM; CLU_070764_0_0_9; -.
DR   OMA; GMCLGYP; -.
DR   PRO; PR:Q2G0Z5; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   CDD; cd02146; NfsA-like; 1.
DR   Gene3D; 3.40.109.10; -; 1.
DR   InterPro; IPR016446; Flavin_OxRdtase_Frp.
DR   InterPro; IPR029479; Nitroreductase.
DR   InterPro; IPR000415; Nitroreductase-like.
DR   PANTHER; PTHR43425; PTHR43425; 1.
DR   Pfam; PF00881; Nitroreductase; 1.
DR   PIRSF; PIRSF005426; Frp; 1.
DR   SUPFAM; SSF55469; SSF55469; 1.
PE   1: Evidence at protein level;
KW   Flavoprotein; FMN; NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..251
FT                   /note="NADPH-dependent oxidoreductase"
FT                   /id="PRO_0000247968"
SQ   SEQUENCE   251 AA;  28597 MW;  1355044E1B8F11F0 CRC64;
     MSEHVYNLVK KHHSVRKFKN KPLSEDVVKK LVEAGQSAST SSFLQAYSII GIDDEKIKEN
     LREVSGQPYV VENGYLFVFV IDYYRHHLVD QHAETDMENA YGSTEGLLVG AIDAALVAEN
     IAVTAEDMGY GIVFLGSLRN DVERVREILD LPDYVFPVFG MAVGEPADDE NGAAKPRLPF
     DHVFHHNKYH ADKETQYAQM ADYDQTISEY YDQRTNGNRK ETWSQQIEMF LGNKARLDML
     EQLQKSGLIQ R