NRFE_ECOLI
ID NRFE_ECOLI Reviewed; 552 AA.
AC P32710; Q2M6N0;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 29-AUG-2003, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Probable cytochrome c-type biogenesis protein NrfE;
GN Name=nrfE; Synonyms=yjcL; OrderedLocusNames=b4074, JW4035;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8057835; DOI=10.1111/j.1365-2958.1994.tb01004.x;
RA Hussain H.A., Grove J., Griffiths L., Busby S., Cole J.;
RT "A seven-gene operon essential for formate-dependent nitrite reduction to
RT ammonia by enteric bacteria.";
RL Mol. Microbiol. 12:153-163(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8265357; DOI=10.1093/nar/21.23.5408;
RA Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.;
RT "Analysis of the Escherichia coli genome. IV. DNA sequence of the region
RT from 89.2 to 92.8 minutes.";
RL Nucleic Acids Res. 21:5408-5417(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / JCB7120;
RX PubMed=9593308; DOI=10.1046/j.1365-2958.1998.00792.x;
RA Eaves D.J., Grove J., Staudenmann W., James P., Poole R.K., White S.A.,
RA Griffiths I., Cole J.A.;
RT "Involvement of products of the nrfEFG genes in the covalent attachment of
RT haem c to a novel cysteine-lysine motif in the cytochrome c552 nitrite
RT reductase from Escherichia coli.";
RL Mol. Microbiol. 28:205-216(1998).
RN [6]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: May be required for the biogenesis of c-type cytochromes.
CC Possible subunit of a heme lyase. {ECO:0000305|PubMed:15919996}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000305|PubMed:15919996}.
CC -!- DISRUPTION PHENOTYPE: Triple nrfE-nrfF-nrfG deletion mutants no longer
CC attach heme 1 (covalently attached to 'Cys-122', the CWSCK motif) of
CC cytochrome c552 (AC P0ABK9). {ECO:0000269|PubMed:9593308}.
CC -!- SIMILARITY: Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family.
CC {ECO:0000305}.
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DR EMBL; X72298; CAA51045.1; -; Genomic_DNA.
DR EMBL; U00006; AAC43168.1; -; Genomic_DNA.
DR EMBL; U00096; AAD13457.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78076.1; -; Genomic_DNA.
DR PIR; A65216; E57987.
DR RefSeq; NP_418498.1; NC_000913.3.
DR AlphaFoldDB; P32710; -.
DR SMR; P32710; -.
DR BioGRID; 4262677; 7.
DR STRING; 511145.b4074; -.
DR PaxDb; P32710; -.
DR PRIDE; P32710; -.
DR EnsemblBacteria; AAD13457; AAD13457; b4074.
DR EnsemblBacteria; BAE78076; BAE78076; BAE78076.
DR GeneID; 948579; -.
DR KEGG; ecj:JW4035; -.
DR KEGG; eco:b4074; -.
DR PATRIC; fig|511145.12.peg.4197; -.
DR EchoBASE; EB1891; -.
DR eggNOG; COG1138; Bacteria.
DR HOGENOM; CLU_015041_3_0_6; -.
DR InParanoid; P32710; -.
DR OMA; EFWLEDN; -.
DR PhylomeDB; P32710; -.
DR BioCyc; EcoCyc:EG11948-MON; -.
DR PRO; PR:P32710; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0015232; F:heme transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0018378; P:cytochrome c-heme linkage via heme-L-cysteine; IMP:EcoCyc.
DR InterPro; IPR032523; CcmF_C.
DR InterPro; IPR002541; Cyt_c_assembly.
DR InterPro; IPR003567; Cyt_c_biogenesis.
DR InterPro; IPR003568; Cyt_c_biogenesis_CcmF.
DR InterPro; IPR003570; Cyt_c_biogenesis_NrfE.
DR PANTHER; PTHR43653; PTHR43653; 1.
DR Pfam; PF16327; CcmF_C; 2.
DR Pfam; PF01578; Cytochrom_C_asm; 1.
DR PRINTS; PR01410; CCBIOGENESIS.
DR PRINTS; PR01413; NRFEBIOGNSIS.
DR TIGRFAMs; TIGR00353; nrfE; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Cytochrome c-type biogenesis; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..552
FT /note="Probable cytochrome c-type biogenesis protein NrfE"
FT /id="PRO_0000201589"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 294..314
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 333..353
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 399..419
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 525..545
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 32
FT /note="L -> V (in Ref. 1; CAA51045)"
FT /evidence="ECO:0000305"
FT CONFLICT 38
FT /note="G -> A (in Ref. 1; CAA51045)"
FT /evidence="ECO:0000305"
FT CONFLICT 190
FT /note="C -> S (in Ref. 1; CAA51045)"
FT /evidence="ECO:0000305"
FT CONFLICT 218
FT /note="W -> L (in Ref. 1; CAA51045)"
FT /evidence="ECO:0000305"
FT CONFLICT 220..226
FT /note="GWWFWDP -> RLVVLGS (in Ref. 1; CAA51045)"
FT /evidence="ECO:0000305"
FT CONFLICT 455
FT /note="G -> A (in Ref. 1; CAA51045)"
FT /evidence="ECO:0000305"
FT CONFLICT 481
FT /note="R -> A (in Ref. 1; CAA51045)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 552 AA; 61295 MW; 138C2E21933699E3 CRC64;
MLTPLTAFAG VRLRWPAMMR LTCIGILAQF ALLLLAFGVL TYCFLISDFS VIYVAQHSYS
LLSWELKLAA VWGGHEGSLL LWVLLLSAWS ALFAWHYRQQ TDPLFPLTLA VLSLMLAALL
LFVVLWSDPF VRIFPPAIEG RDLNPMLQHP GLIFHPPLLY LGYGGLMVAA SVALASLLRG
EFDGACARIC WRWALPGWSA LTAGIILGSW WAYCELGWGG WWFWDPVENA SLLPWLSATA
LLHSLSLTRQ RGIFCHWSLL LAIVTLMLSL LGTLIVRSGI LVSVHAFALD NVRAVPLFSL
FALISLASLA LYGWRARDGG PAVHFSGLSR EMLILATLLL FCAVLLIVLV GTLYPMIYGL
LGWGRLSVGA PYFNRATLPF GLLMLVVIVL ATFVSGKRVQ LPALVAHAGV LLFAAGVVVS
SVSRQEISLN LQPGQQVTLA GYTFRFECLD LQAKGNYTSE KAIVALFDHQ QRIGELTPER
RFYEARRQQM MEPSIRWNGI HDWYAVMGEK TGPDRYAFRL YVQSGVRWIW GGGLLMIAGA
LLSGWRGKKR DE
