ID   NRFF_ECO57              Reviewed;         127 AA.
AC   Q8X5S5;
DT   06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Formate-dependent nitrite reductase complex subunit NrfF;
DE   Flags: Precursor;
GN   Name=nrfF; OrderedLocusNames=Z5674, ECs5057;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Not required for the biosynthesis of any of the c-type
CC       cytochromes. Possible subunit of a heme lyase.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the CcmH/CycL/Ccl2/NrfF family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG59273.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB38480.1; -; Genomic_DNA.
DR   PIR; A98261; A98261.
DR   PIR; E86101; E86101.
DR   RefSeq; NP_313084.1; NC_002695.1.
DR   RefSeq; WP_000276458.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; Q8X5S5; -.
DR   SMR; Q8X5S5; -.
DR   STRING; 155864.EDL933_5415; -.
DR   EnsemblBacteria; AAG59273; AAG59273; Z5674.
DR   EnsemblBacteria; BAB38480; BAB38480; ECs_5057.
DR   GeneID; 914285; -.
DR   KEGG; ece:Z5674; -.
DR   KEGG; ecs:ECs_5057; -.
DR   PATRIC; fig|386585.9.peg.5283; -.
DR   eggNOG; COG3088; Bacteria.
DR   HOGENOM; CLU_107187_0_2_6; -.
DR   OMA; INEIRCP; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.8.640; -; 1.
DR   InterPro; IPR005616; CcmH/CycL/Ccl2/NrfF.
DR   InterPro; IPR038297; CcmH/CycL/NrfF/Ccl2_sf.
DR   InterPro; IPR017565; For-dep_Cytc_NO2Rdtase_NrfF.
DR   Pfam; PF03918; CcmH; 1.
DR   TIGRFAMs; TIGR03147; cyt_nit_nrfF; 1.
PE   3: Inferred from homology;
KW   Heme; Iron; Metal-binding; Periplasm; Reference proteome; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..127
FT                   /note="Formate-dependent nitrite reductase complex subunit
FT                   NrfF"
FT                   /id="PRO_0000006615"
FT   BINDING         44
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /note="covalent"
FT                   /evidence="ECO:0000255"
FT   BINDING         47
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /note="covalent"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   127 AA;  14564 MW;  12529B2DABF69637 CRC64;
     MYKGLLTLLL LFTCFARAQV VDTWQFANPQ QQQQALNIAS QLRCPQCQNQ NLLESNAPVA
     VSMRHQVYSM VAEGKSEVEI IGWMTERYGD FVRYNPPLTG QTLVLWALPV VLLLLMALIL
     WRVRAKR