NRFF_ECOLI
ID NRFF_ECOLI Reviewed; 127 AA.
AC P32711; Q2M6M9;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Formate-dependent nitrite reductase complex subunit NrfF;
DE Flags: Precursor;
GN Name=nrfF; Synonyms=yjcM; OrderedLocusNames=b4075, JW4036;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8057835; DOI=10.1111/j.1365-2958.1994.tb01004.x;
RA Hussain H.A., Grove J., Griffiths L., Busby S., Cole J.;
RT "A seven-gene operon essential for formate-dependent nitrite reduction to
RT ammonia by enteric bacteria.";
RL Mol. Microbiol. 12:153-163(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8265357; DOI=10.1093/nar/21.23.5408;
RA Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.;
RT "Analysis of the Escherichia coli genome. IV. DNA sequence of the region
RT from 89.2 to 92.8 minutes.";
RL Nucleic Acids Res. 21:5408-5417(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP POSSIBLE FUNCTION.
RC STRAIN=K12;
RX PubMed=8842153; DOI=10.1007/bf02173779;
RA Grovc J., Busby S., Cole J.;
RT "The role of the genes nrf EFG and ccmFH in cytochrome c biosynthesis in
RT Escherichia coli.";
RL Mol. Gen. Genet. 252:332-341(1996).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / JCB7120;
RX PubMed=9593308; DOI=10.1046/j.1365-2958.1998.00792.x;
RA Eaves D.J., Grove J., Staudenmann W., James P., Poole R.K., White S.A.,
RA Griffiths I., Cole J.A.;
RT "Involvement of products of the nrfEFG genes in the covalent attachment of
RT haem c to a novel cysteine-lysine motif in the cytochrome c552 nitrite
RT reductase from Escherichia coli.";
RL Mol. Microbiol. 28:205-216(1998).
CC -!- FUNCTION: Not required for the biosynthesis of any of the c-type
CC cytochromes. Possible subunit of a heme lyase.
CC {ECO:0000305|PubMed:9593308}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Triple nrfE-nrfF-nrfG deletion mutants no longer
CC attach heme 1 (covalently attached to 'Cys-122', the CWSCK motif) of
CC cytochrome c552 (AC P0ABK9). {ECO:0000269|PubMed:9593308}.
CC -!- SIMILARITY: Belongs to the CcmH/CycL/Ccl2/NrfF family. {ECO:0000305}.
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DR EMBL; X72298; CAA51046.1; -; Genomic_DNA.
DR EMBL; U00006; AAC43169.1; -; Genomic_DNA.
DR EMBL; U00096; AAD13458.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78077.1; -; Genomic_DNA.
DR PIR; F57987; F57987.
DR RefSeq; NP_418499.1; NC_000913.3.
DR RefSeq; WP_001032531.1; NZ_SSZK01000016.1.
DR AlphaFoldDB; P32711; -.
DR SMR; P32711; -.
DR BioGRID; 4262678; 9.
DR STRING; 511145.b4075; -.
DR PaxDb; P32711; -.
DR PRIDE; P32711; -.
DR EnsemblBacteria; AAD13458; AAD13458; b4075.
DR EnsemblBacteria; BAE78077; BAE78077; BAE78077.
DR GeneID; 948578; -.
DR KEGG; ecj:JW4036; -.
DR KEGG; eco:b4075; -.
DR PATRIC; fig|1411691.4.peg.2629; -.
DR EchoBASE; EB1892; -.
DR eggNOG; COG3088; Bacteria.
DR HOGENOM; CLU_107187_0_2_6; -.
DR InParanoid; P32711; -.
DR OMA; INEIRCP; -.
DR PhylomeDB; P32711; -.
DR BioCyc; EcoCyc:EG11949-MON; -.
DR PRO; PR:P32711; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0018378; P:cytochrome c-heme linkage via heme-L-cysteine; IMP:EcoCyc.
DR Gene3D; 1.10.8.640; -; 1.
DR InterPro; IPR005616; CcmH/CycL/Ccl2/NrfF.
DR InterPro; IPR038297; CcmH/CycL/NrfF/Ccl2_sf.
DR InterPro; IPR017565; For-dep_Cytc_NO2Rdtase_NrfF.
DR Pfam; PF03918; CcmH; 1.
DR TIGRFAMs; TIGR03147; cyt_nit_nrfF; 1.
PE 3: Inferred from homology;
KW Heme; Iron; Metal-binding; Periplasm; Reference proteome; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..127
FT /note="Formate-dependent nitrite reductase complex subunit
FT NrfF"
FT /id="PRO_0000006614"
FT BINDING 44
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /note="covalent"
FT /evidence="ECO:0000255"
FT BINDING 47
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /note="covalent"
FT /evidence="ECO:0000255"
SQ SEQUENCE 127 AA; 14523 MW; AFE4404B9A398231 CRC64;
MNKGLLTLLL LFTCFAHAQV VDTWQFANPQ QQQQALNIAS QLRCPQCQNQ NLLESNAPVA
VSMRHQVYSM VAEGKNEVEI IGWMTERYGD FVRYNPPLTG QTLVLWALPV VLLLLMALIL
WRVRAKR
