NRFG_ECOLI
ID NRFG_ECOLI Reviewed; 198 AA.
AC P32712; Q2M6M8;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Formate-dependent nitrite reductase complex subunit NrfG;
GN Name=nrfG; Synonyms=yjcN; OrderedLocusNames=b4076, JW4037;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8057835; DOI=10.1111/j.1365-2958.1994.tb01004.x;
RA Hussain H.A., Grove J., Griffiths L., Busby S., Cole J.;
RT "A seven-gene operon essential for formate-dependent nitrite reduction to
RT ammonia by enteric bacteria.";
RL Mol. Microbiol. 12:153-163(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8265357; DOI=10.1093/nar/21.23.5408;
RA Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.;
RT "Analysis of the Escherichia coli genome. IV. DNA sequence of the region
RT from 89.2 to 92.8 minutes.";
RL Nucleic Acids Res. 21:5408-5417(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP POSSIBLE FUNCTION.
RC STRAIN=K12;
RX PubMed=8842153; DOI=10.1007/bf02173779;
RA Grovc J., Busby S., Cole J.;
RT "The role of the genes nrf EFG and ccmFH in cytochrome c biosynthesis in
RT Escherichia coli.";
RL Mol. Gen. Genet. 252:332-341(1996).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / JCB7120;
RX PubMed=9593308; DOI=10.1046/j.1365-2958.1998.00792.x;
RA Eaves D.J., Grove J., Staudenmann W., James P., Poole R.K., White S.A.,
RA Griffiths I., Cole J.A.;
RT "Involvement of products of the nrfEFG genes in the covalent attachment of
RT haem c to a novel cysteine-lysine motif in the cytochrome c552 nitrite
RT reductase from Escherichia coli.";
RL Mol. Microbiol. 28:205-216(1998).
CC -!- FUNCTION: Required for formate-dependent nitrite reduction. Not
CC required for the biosynthesis of any of the c-type cytochromes nor for
CC the secretion of the periplasmic cytochromes.
CC {ECO:0000305|PubMed:9593308}.
CC -!- DISRUPTION PHENOTYPE: Triple nrfE-nrfF-nrfG deletion mutants no longer
CC attach heme 1 (covalently attached to 'Cys-122', the CWSCK motif) of
CC cytochrome c552 (AC P0ABK9). {ECO:0000269|PubMed:9593308}.
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DR EMBL; X72298; CAA51047.1; -; Genomic_DNA.
DR EMBL; U00006; AAC43170.1; -; Genomic_DNA.
DR EMBL; U00096; AAD13459.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78078.1; -; Genomic_DNA.
DR PIR; A57987; A57987.
DR RefSeq; NP_418500.1; NC_000913.3.
DR RefSeq; WP_000812896.1; NZ_SSZK01000016.1.
DR AlphaFoldDB; P32712; -.
DR SMR; P32712; -.
DR BioGRID; 4261973; 10.
DR BioGRID; 852886; 1.
DR IntAct; P32712; 5.
DR STRING; 511145.b4076; -.
DR PaxDb; P32712; -.
DR PRIDE; P32712; -.
DR EnsemblBacteria; AAD13459; AAD13459; b4076.
DR EnsemblBacteria; BAE78078; BAE78078; BAE78078.
DR GeneID; 948592; -.
DR KEGG; ecj:JW4037; -.
DR KEGG; eco:b4076; -.
DR PATRIC; fig|1411691.4.peg.2628; -.
DR EchoBASE; EB1893; -.
DR eggNOG; COG4235; Bacteria.
DR HOGENOM; CLU_036074_3_1_6; -.
DR InParanoid; P32712; -.
DR OMA; ALICFDY; -.
DR PhylomeDB; P32712; -.
DR BioCyc; EcoCyc:EG11950-MON; -.
DR PRO; PR:P32712; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0018378; P:cytochrome c-heme linkage via heme-L-cysteine; IMP:EcoCyc.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR SMART; SM00028; TPR; 2.
DR SUPFAM; SSF48452; SSF48452; 1.
DR PROSITE; PS50005; TPR; 2.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 4: Predicted;
KW Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..198
FT /note="Formate-dependent nitrite reductase complex subunit
FT NrfG"
FT /id="PRO_0000106433"
FT REPEAT 73..106
FT /note="TPR 1"
FT REPEAT 144..177
FT /note="TPR 2"
SQ SEQUENCE 198 AA; 22784 MW; 4F7E369ABC5949C3 CRC64;
MKQPKIPVKM LTTLTILMVF LCVGSYLLSP KWQAVRAEYQ RQRDPLHQFA SQQTPEAQLQ
ALQDKIRANP QNSEQWALLG EYYLWQNDYS NSLLAYRQAL QLRGENAELY AALATVLYYQ
ASQHMTAQTR AMIDKALALD SNEITALMLL ASDAFMQANY AQAIELWQKV MDLNSPRVNR
TQLVESINMA KLLQRRLD
