NRG2_ARATH
ID NRG2_ARATH Reviewed; 796 AA.
AC Q93YU8; Q56XC8; Q9LY33;
DT 27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Nitrate regulatory gene2 protein {ECO:0000303|PubMed:26744214};
DE Short=NRG2 protein {ECO:0000303|PubMed:26744214};
GN Name=NRG2 {ECO:0000303|PubMed:26744214};
GN OrderedLocusNames=At3g60320 {ECO:0000312|Araport:AT3G60320,
GN ECO:0000312|EMBL:AEE80044.1, ECO:0000312|EMBL:BAD93791.1};
GN ORFNames=F27H5_110 {ECO:0000312|EMBL:CAB87869.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAL24107.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-534.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, INTERACTION WITH NLP7, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=26744214; DOI=10.1105/tpc.15.00567;
RA Xu N., Wang R., Zhao L., Zhang C., Li Z., Lei Z., Liu F., Guan P., Chu Z.,
RA Crawford N.M., Wang Y.;
RT "The Arabidopsis NRG2 protein mediates nitrate signaling and interacts with
RT and regulates key nitrate regulators.";
RL Plant Cell 28:485-504(2016).
CC -!- FUNCTION: Required for nitrate signaling. Regulates expression of the
CC nitrate-responsive genes NIA1, NIR1, NRT2.1 and NPF6.3/NRT1.1.
CC {ECO:0000269|PubMed:26744214}.
CC -!- SUBUNIT: Interacts with NLP7. {ECO:0000269|PubMed:26744214}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:26744214}.
CC -!- TISSUE SPECIFICITY: Highest expression levels in leaves and roots, and
CC lowest in flowers and siliques. Also expressed in stems and seedlings.
CC Also expressed in stromata. In flowers, expressed in the pistil,
CC junction of filament and anther, and vascular tissue of sepals and
CC petals. In roots, expressed in stelar cells including the pericycle,
CC phloem and parenchyma cells. In leaves, expressed in bundle sheath,
CC phloem and parenchyma cells of the vascular tissue.
CC {ECO:0000269|PubMed:26744214}.
CC -!- DISRUPTION PHENOTYPE: Under high nitrate concentration, seedlings are
CC slightly smaller and display later flowering than wild-type. Under low
CC nitrate concentration, seedlings appear normal. Nitrate accumulation of
CC the seedlings and roots is significantly lower than in wild-type,
CC however no difference of nitrate accumulation in leaves. Nitrate
CC content differences in roots and leaves may be due to the reduced
CC expression of NPF6.3/NRT1.1 in roots and the increased expression of
CC NPF7.2/NRT1.8 in leaves. After nitrate treatment, altered expression of
CC many genes involved in nitrogen-related clusters including nitrate
CC transport and response to nitrate. {ECO:0000269|PubMed:26744214}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB87869.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL163852; CAB87869.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002686; AEE80044.1; -; Genomic_DNA.
DR EMBL; AY059759; AAL24107.1; -; mRNA.
DR EMBL; AK221747; BAD93791.1; -; mRNA.
DR PIR; T49227; T49227.
DR RefSeq; NP_191591.2; NM_115896.3.
DR AlphaFoldDB; Q93YU8; -.
DR SMR; Q93YU8; -.
DR STRING; 3702.AT3G60320.1; -.
DR iPTMnet; Q93YU8; -.
DR PaxDb; Q93YU8; -.
DR PRIDE; Q93YU8; -.
DR ProteomicsDB; 250564; -.
DR EnsemblPlants; AT3G60320.1; AT3G60320.1; AT3G60320.
DR GeneID; 825203; -.
DR Gramene; AT3G60320.1; AT3G60320.1; AT3G60320.
DR KEGG; ath:AT3G60320; -.
DR Araport; AT3G60320; -.
DR TAIR; locus:2081922; AT3G60320.
DR eggNOG; ENOG502QU01; Eukaryota.
DR HOGENOM; CLU_010985_5_0_1; -.
DR InParanoid; Q93YU8; -.
DR OMA; MWKSMNQ; -.
DR OrthoDB; 482517at2759; -.
DR PhylomeDB; Q93YU8; -.
DR PRO; PR:Q93YU8; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q93YU8; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0071249; P:cellular response to nitrate; IMP:TAIR.
DR GO; GO:0015706; P:nitrate transmembrane transport; IMP:TAIR.
DR GO; GO:0071705; P:nitrogen compound transport; IEP:TAIR.
DR GO; GO:0010167; P:response to nitrate; IEP:TAIR.
DR GO; GO:1901698; P:response to nitrogen compound; IEP:TAIR.
DR InterPro; IPR006868; DUF630.
DR InterPro; IPR006867; DUF632.
DR Pfam; PF04783; DUF630; 1.
DR Pfam; PF04782; DUF632; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..796
FT /note="Nitrate regulatory gene2 protein"
FT /id="PRO_0000441624"
FT REGION 57..174
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 190..342
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 227..255
FT /evidence="ECO:0000255"
FT COMPBIAS 71..103
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 104..126
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 139..174
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 196..247
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 255..271
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 290..310
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 371
FT /note="A -> D (in Ref. 4; BAD93791)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 796 AA; 89979 MW; A893773CF978E490 CRC64;
MGCAASKLDN EDAVRRCKDR RRLMKEAVYA RHHLAAAHAD YCRSLRITGS ALSSFASGEP
LSVSDQTPAV FLHTPPPPLS EQSPAKFVPP RFSPSPAPSS VYPPSTSPSV ASSKQPSVMS
TSSNRRRKQQ PKPRLPHILS ESSPSSSPRS ERSNFMPNLY PSAYQNSTYS ATPSHASSVW
NWENFYPPSP PDSEFFNRKA QEKKHNSDNR FNDEDTETVR SEYDFFDTRK QKQKQFESMR
NQVEEETETE REEVQCSEWE DHDHYSTTSS SDAAEEEEED DDRESISEVG TRSEFGSTVR
SNSMRRHHQQ PSPMPQVYGG AEQSKYDKAD DATISSGSYR GGGDIADMKM VVRHRDLKEI
IDAIKENFDK AAASGEQVSQ MLELGRAELD RSFSQLKKTV IHSSSLLSNL SSTWTSKPPL
AVKYRIDTTA LDQPNSSKSL CSTLDRLLAW EKKLYEEIKA REGFKIEHEK KLSQLQSQEY
KGEDEAKLDK TKASITRLQS LIIVTSQAVT TTSTAIIRLR DTDLVPQLVE LCHGFMYMWK
SMHQYHETQN SIVEQVRGLI NRSGKGESTS ELHRQATRDL ESAVSSWHSS FSSLIKFQRD
FIHSVHAWFK LTLLPVCQED AANHHKEPLD AYAFCDEWKL ALDRIPDTVA SEAIKSFINV
VHVISAKQAD EHKIKKRTES ASKELEKKAS SVRNLERKYY QSYSMVGVGL PESGPDNQHM
LDARDPLSDK KSELAVCQRR VEEEMVKYSK AIEVTRAMTL NNLQTGLPGV FQSLTSFSAL
FMESLQTVCT RSYSIK
