NRGB_BACSU
ID NRGB_BACSU Reviewed; 116 AA.
AC Q07428;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Nitrogen regulatory PII-like protein;
GN Name=nrgB; OrderedLocusNames=BSU36520;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8282685; DOI=10.1128/jb.176.1.108-114.1994;
RA Wray L.V. Jr., Atkinson M.R., Fisher S.H.;
RT "The nitrogen-regulated Bacillus subtilis nrgAB operon encodes a membrane
RT protein and a protein highly similar to the Escherichia coli glnB-encoded
RT PII protein.";
RL J. Bacteriol. 176:108-114(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9353933; DOI=10.1099/00221287-143-10-3313;
RA Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V.,
RA Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G.,
RA Kunst F., Danchin A., Glaser P.;
RT "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334
RT degrees).";
RL Microbiology 143:3313-3328(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RC STRAIN=168;
RX PubMed=14600241; DOI=10.1099/mic.0.26512-0;
RA Detsch C., Stuelke J.;
RT "Ammonium utilization in Bacillus subtilis: transport and regulatory
RT functions of NrgA and NrgB.";
RL Microbiology 149:3289-3297(2003).
RN [5]
RP INDUCTION BY TNRA.
RX PubMed=12823818; DOI=10.1046/j.1365-2958.2003.03567.x;
RA Yoshida K., Yamaguchi H., Kinehara M., Ohki Y.-H., Nakaura Y., Fujita Y.;
RT "Identification of additional TnrA-regulated genes of Bacillus subtilis
RT associated with a TnrA box.";
RL Mol. Microbiol. 49:157-165(2003).
RN [6]
RP REGULATION OF THE NRGAB OPERON BY TNRA.
RC STRAIN=168;
RX PubMed=10864496; DOI=10.1006/jmbi.2000.3846;
RA Wray L.V. Jr., Zalieckas J.M., Fisher S.H.;
RT "Purification and in vitro activities of the Bacillus subtilis TnrA
RT transcription factor.";
RL J. Mol. Biol. 300:29-40(2000).
CC -!- FUNCTION: Required for full induction of the nrgAB operon under
CC conditions of ammonium limitation. {ECO:0000269|PubMed:14600241}.
CC -!- SUBUNIT: Needs to interact with NrgA in order to localize correctly to
CC the membrane.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:14600241};
CC Peripheral membrane protein {ECO:0000269|PubMed:14600241}.
CC -!- INDUCTION: The nrgAB operon is activated by TnrA under nitrogen-limited
CC conditions. {ECO:0000269|PubMed:12823818}.
CC -!- DISRUPTION PHENOTYPE: Cells show no growth phenotype even at acidic pH.
CC {ECO:0000269|PubMed:14600241}.
CC -!- SIMILARITY: Belongs to the P(II) protein family. {ECO:0000255|PROSITE-
CC ProRule:PRU00675}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-5 is the initiator.
CC {ECO:0000305}.
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DR EMBL; L03216; AAA17400.1; -; Unassigned_DNA.
DR EMBL; Z82987; CAB05373.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15669.1; -; Genomic_DNA.
DR PIR; B36865; B36865.
DR RefSeq; NP_391533.1; NC_000964.3.
DR RefSeq; WP_003221829.1; NZ_JNCM01000034.1.
DR PDB; 4R25; X-ray; 2.52 A; A=5-116.
DR PDB; 4RX6; X-ray; 2.60 A; A/B/D=1-116.
DR PDBsum; 4R25; -.
DR PDBsum; 4RX6; -.
DR AlphaFoldDB; Q07428; -.
DR SMR; Q07428; -.
DR STRING; 224308.BSU36520; -.
DR PaxDb; Q07428; -.
DR PRIDE; Q07428; -.
DR EnsemblBacteria; CAB15669; CAB15669; BSU_36520.
DR GeneID; 64305421; -.
DR GeneID; 936937; -.
DR KEGG; bsu:BSU36520; -.
DR PATRIC; fig|224308.179.peg.3952; -.
DR eggNOG; COG0347; Bacteria.
DR InParanoid; Q07428; -.
DR OMA; HQIEVNF; -.
DR PhylomeDB; Q07428; -.
DR BioCyc; BSUB:BSU36520-MON; -.
DR PRO; PR:Q07428; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IBA:GO_Central.
DR GO; GO:0030234; F:enzyme regulator activity; IBA:GO_Central.
DR GO; GO:0006808; P:regulation of nitrogen utilization; IBA:GO_Central.
DR Gene3D; 3.30.70.120; -; 1.
DR InterPro; IPR002187; N-reg_PII.
DR InterPro; IPR011322; N-reg_PII-like_a/b.
DR InterPro; IPR015867; N-reg_PII/ATP_PRibTrfase_C.
DR InterPro; IPR017918; N-reg_PII_CS.
DR PANTHER; PTHR30115; PTHR30115; 1.
DR Pfam; PF00543; P-II; 1.
DR PRINTS; PR00340; PIIGLNB.
DR SMART; SM00938; P-II; 1.
DR SUPFAM; SSF54913; SSF54913; 1.
DR PROSITE; PS00638; PII_GLNB_CTER; 1.
DR PROSITE; PS51343; PII_GLNB_DOM; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Cell membrane; Membrane; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..116
FT /note="Nitrogen regulatory PII-like protein"
FT /id="PRO_0000139814"
FT STRAND 6..12
FT /evidence="ECO:0007829|PDB:4R25"
FT HELIX 17..27
FT /evidence="ECO:0007829|PDB:4R25"
FT STRAND 33..46
FT /evidence="ECO:0007829|PDB:4R25"
FT HELIX 48..50
FT /evidence="ECO:0007829|PDB:4RX6"
FT STRAND 53..72
FT /evidence="ECO:0007829|PDB:4R25"
FT HELIX 74..85
FT /evidence="ECO:0007829|PDB:4R25"
FT STRAND 88..90
FT /evidence="ECO:0007829|PDB:4R25"
FT STRAND 94..99
FT /evidence="ECO:0007829|PDB:4R25"
FT STRAND 101..105
FT /evidence="ECO:0007829|PDB:4RX6"
FT TURN 106..108
FT /evidence="ECO:0007829|PDB:4R25"
FT HELIX 112..114
FT /evidence="ECO:0007829|PDB:4R25"
SQ SEQUENCE 116 AA; 12822 MW; 386FCB5C1133CC59 CRC64;
MSGQMFKVEI VTRPANFEKL KQELGKIGVT SLTFSNVHGC GLQKAHTELY RGVKIESNVY
ERLKIEIVVS KVPVDQVTET AKRVLKTGSP GDGKIFVYEI SNTINIRTGE EGPEAL
