ID   NRKB_TRYBB              Reviewed;         431 AA.
AC   Q03428;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Putative serine/threonine-protein kinase B;
DE            EC=2.7.11.1;
GN   Name=NRKB;
OS   Trypanosoma brucei brucei.
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=5702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Isolate TREU66;
RX   PubMed=8515773; DOI=10.1016/0166-6851(93)90012-m;
RA   Gale M.J. Jr., Parsons M.;
RT   "A Trypanosoma brucei gene family encoding protein kinases with catalytic
RT   domains structurally related to Nek1 and NIMA.";
RL   Mol. Biochem. Parasitol. 59:111-122(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC         [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC         threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC         Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC         EC=2.7.11.1;
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC       kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
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DR   EMBL; L03777; AAB59253.1; -; mRNA.
DR   AlphaFoldDB; Q03428; -.
DR   SMR; Q03428; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR   GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR   Gene3D; 2.30.29.30; -; 1.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR001849; PH_domain.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   InterPro; IPR008271; Ser/Thr_kinase_AS.
DR   Pfam; PF00169; PH; 1.
DR   Pfam; PF00069; Pkinase; 1.
DR   SMART; SM00233; PH; 1.
DR   SMART; SM00220; S_TKc; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   PROSITE; PS50003; PH_DOMAIN; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR   PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Kinase; Nucleotide-binding; Serine/threonine-protein kinase;
KW   Transferase.
FT   CHAIN           1..431
FT                   /note="Putative serine/threonine-protein kinase B"
FT                   /id="PRO_0000086448"
FT   DOMAIN          20..279
FT                   /note="Protein kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   DOMAIN          331..429
FT                   /note="PH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT   ACT_SITE        147
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT                   ECO:0000255|PROSITE-ProRule:PRU10027"
FT   BINDING         26..34
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   BINDING         49
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ   SEQUENCE   431 AA;  48172 MW;  F6B4B3EF9E99FF74 CRC64;
     MSEPFSTILG TDGSGGRCKY LNKGIVGLGS YGEGYVAERV EDGSLCVAKV MDLSKMSRRD
     KRYAQSEIKY PTNCNHPNII RYIEDHEEND RLLIVMEFAD SGNLDEQIKP WGTGDARYFQ
     EHEALFLFLQ LCLALDYIHS HKMLHRDIKS ANVLLTSTGL VKLGDFGFSH QYEDTVSGVV
     ASTFCGTPYY LAPELWNNLR YNKKADVWSL GVLLYEIMGM KKPFSASNLK GLMSKVLAGT
     YAPLPDSFSS EFKRVVDGIL VADPNDRPSV RENFQIPYIN KGLKLFVQAL KKNERILDSV
     KEVLVSQVSE ILSSEVSPDA HRFLESQINY DVTHRGHVNK LGGGNGKSWK PRFLQIVRGQ
     LILTDDEEGN NPKGLNLEQV QGACPVPYST AKRDFVFALN TVGGEGMWFQ AVSHGDMEMW
     VHAIQRGIGV A