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NRL4_ORYSJ
ID   NRL4_ORYSJ              Reviewed;         362 AA.
AC   Q6H849; A3A9D2; B7EVI5; Q9SXX6;
DT   14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Bifunctional nitrilase/nitrile hydratase NIT4;
DE            Short=OsNIT4;
DE            EC=3.5.5.1;
DE            EC=3.5.5.4;
DE   AltName: Full=Cyanoalanine nitrilase;
DE   AltName: Full=Nitrilase 4;
GN   Name=NIT4; OrderedLocusNames=Os02g0635200, LOC_Os02g42350;
GN   ORFNames=OJ1626_B09.8, OsJ_007404;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Nipponbare; TISSUE=Leaf;
RA   Chiba R., Dohmoto M., Yamaguchi K.;
RT   "Oryza sativa, a gene for nitrilase-like protein.";
RL   Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Highly specific for beta-cyano-L-alanine (Ala(CN)). Low
CC       activity with 3-phenylpropionitrile (PPN). Not associated with auxin
CC       production but may be involved in cyanide detoxification (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a nitrile + 2 H2O = a carboxylate + NH4(+);
CC         Xref=Rhea:RHEA:21724, ChEBI:CHEBI:15377, ChEBI:CHEBI:18379,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:29067; EC=3.5.5.1;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10105};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-cyano-L-alanine + 2 H2O = L-aspartate + NH4(+);
CC         Xref=Rhea:RHEA:11188, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:29991, ChEBI:CHEBI:77860; EC=3.5.5.4;
CC   -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC       Nitrilase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAZ23921.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB027054; BAA77679.1; -; mRNA.
DR   EMBL; AP004069; BAD25100.1; -; Genomic_DNA.
DR   EMBL; AP008208; BAF09432.1; -; Genomic_DNA.
DR   EMBL; AP014958; BAS79936.1; -; Genomic_DNA.
DR   EMBL; CM000139; EAZ23921.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AK104033; BAG96382.1; -; mRNA.
DR   EMBL; AK121244; BAH00392.1; -; mRNA.
DR   PIR; T52266; T52266.
DR   RefSeq; XP_015622782.1; XM_015767296.1.
DR   AlphaFoldDB; Q6H849; -.
DR   SMR; Q6H849; -.
DR   STRING; 4530.OS02T0635200-01; -.
DR   PaxDb; Q6H849; -.
DR   PRIDE; Q6H849; -.
DR   EnsemblPlants; Os02t0635200-01; Os02t0635200-01; Os02g0635200.
DR   GeneID; 4330077; -.
DR   Gramene; Os02t0635200-01; Os02t0635200-01; Os02g0635200.
DR   KEGG; osa:4330077; -.
DR   eggNOG; KOG0805; Eukaryota.
DR   HOGENOM; CLU_030130_6_1_1; -.
DR   InParanoid; Q6H849; -.
DR   OMA; PKGLDFG; -.
DR   OrthoDB; 1001274at2759; -.
DR   PlantReactome; R-OSA-1119486; IAA biosynthesis I.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000007752; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   Genevisible; Q6H849; OS.
DR   GO; GO:0047427; F:cyanoalanine nitrilase activity; IEA:UniProtKB-EC.
DR   GO; GO:0080061; F:indole-3-acetonitrile nitrilase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000257; F:nitrilase activity; IBA:GO_Central.
DR   GO; GO:0018822; F:nitrile hydratase activity; IBA:GO_Central.
DR   GO; GO:0051410; P:detoxification of nitrogen compound; IBA:GO_Central.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   CDD; cd07564; nitrilases_CHs; 1.
DR   Gene3D; 3.60.110.10; -; 1.
DR   InterPro; IPR003010; C-N_Hydrolase.
DR   InterPro; IPR036526; C-N_Hydrolase_sf.
DR   InterPro; IPR000132; Nitrilase/CN_hydratase_CS.
DR   InterPro; IPR044149; Nitrilases_CHs.
DR   PANTHER; PTHR46044; PTHR46044; 1.
DR   Pfam; PF00795; CN_hydrolase; 1.
DR   SUPFAM; SSF56317; SSF56317; 1.
DR   PROSITE; PS50263; CN_HYDROLASE; 1.
DR   PROSITE; PS00920; NITRIL_CHT_1; 1.
DR   PROSITE; PS00921; NITRIL_CHT_2; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..362
FT                   /note="Bifunctional nitrilase/nitrile hydratase NIT4"
FT                   /id="PRO_0000352513"
FT   DOMAIN          31..307
FT                   /note="CN hydrolase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        71
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        162
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        196
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054,
FT                   ECO:0000255|PROSITE-ProRule:PRU10105"
FT   CONFLICT        25
FT                   /note="D -> T (in Ref. 1; BAA77679)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        263
FT                   /note="S -> T (in Ref. 1; BAA77679)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   362 AA;  38676 MW;  5D3CA71FFD95FDE0 CRC64;
     MAMVPSGSGG GPPVIAEVEM NGGADSGAAT VRATVVQAST VFYDTPATLD KAERLIEEAA
     GYGSQLVVFP EAFVGGYPRG STFGFGANIS IGNPKDKGKE EFRKYHAAAI EVPGPEVTRL
     AAMAGKYKVF LVMGVIEREG YTLYCSVLFF DPLGRYLGKH RKLMPTALER IIWGFGDGST
     IPVYDTPLGK IGALICWENK MPLLRTALYG KGIEIYCAPT ADSRQVWQAS MTHIALEGGC
     FVLSANQFCR RKDYPPPPEY VFSGLGEEPS PDTVVCPGGS VIISPSGEVL AGPNYEGEAL
     ITADLDLGEI VRAKFDFDVV GHYARPEVLS LVVNDQPHLP VSFTSAAEKT TAAKSDSTAK
     PY
 
 
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