ID   NRLA_ACISA              Reviewed;          21 AA.
AC   P33036;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Nitrilase;
DE            EC=3.5.5.1;
DE   Flags: Fragment;
OS   Acinetobacter sp. (strain AK226).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC   Acinetobacter.
OX   NCBI_TaxID=72569;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=1369128; DOI=10.1271/bbb1961.55.1459;
RA   Yamamoto K., Komatsu K.;
RT   "Purification and characterization of nitrilase responsible for the
RT   enantioselective hydrolysis from Acinetobacter sp. AK 226.";
RL   Agric. Biol. Chem. 55:1459-1466(1991).
CC   -!- FUNCTION: Acts on many kinds of nitrile compounds such as aliphatic,
CC       aromatic, and heterocyclic mononitriles or dinitriles. Prefers S-(-)-2-
CC       (4'-isobutylphenyl)-propionitrile to R-(+)-2-(4'-isobutylphenyl)-
CC       propionitrile as the substrate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a nitrile + 2 H2O = a carboxylate + NH4(+);
CC         Xref=Rhea:RHEA:21724, ChEBI:CHEBI:15377, ChEBI:CHEBI:18379,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:29067; EC=3.5.5.1;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10105};
CC   -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC       Nitrilase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; P33036; -.
DR   GO; GO:0080061; F:indole-3-acetonitrile nitrilase activity; IEA:UniProtKB-EC.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase.
FT   CHAIN           1..>21
FT                   /note="Nitrilase"
FT                   /id="PRO_0000204041"
FT   NON_TER         21
SQ   SEQUENCE   21 AA;  2223 MW;  5FA741C41EAC619B CRC64;
     VSYNSKFLAA TVQAEPVVLD A