ID   NRLA_ALCFA              Reviewed;         356 AA.
AC   P20960;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 2.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Nitrilase, arylacetone-specific;
DE            EC=3.5.5.1;
DE   AltName: Full=Arylacetonitrilase;
OS   Alcaligenes faecalis.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Alcaligenes.
OX   NCBI_TaxID=511;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, AND
RP   MUTAGENESIS OF CYS-162 AND CYS-163.
RC   STRAIN=JM3;
RX   PubMed=8419930; DOI=10.1073/pnas.90.1.247;
RA   Kobayashi M., Izui H., Nagasawa T., Yamada H.;
RT   "Nitrilase in biosynthesis of the plant hormone indole-3-acetic acid from
RT   indole-3-acetonitrile: cloning of the Alcaligenes gene and site-directed
RT   mutagenesis of cysteine residues.";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:247-251(1993).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-33.
RC   STRAIN=JM3;
RX   PubMed=2269298; DOI=10.1111/j.1432-1033.1990.tb19467.x;
RA   Nagasawa T., Mauger J., Yamada H.;
RT   "A novel nitrilase, arylacetonitrilase, of Alcaligenes faecalis JM3.
RT   Purification and characterization.";
RL   Eur. J. Biochem. 194:765-772(1990).
CC   -!- FUNCTION: Nitrilase that acts mostly on arylacetonitriles.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a nitrile + 2 H2O = a carboxylate + NH4(+);
CC         Xref=Rhea:RHEA:21724, ChEBI:CHEBI:15377, ChEBI:CHEBI:18379,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:29067; EC=3.5.5.1;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10105};
CC   -!- SUBUNIT: Homohexamer. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC       Nitrilase family. {ECO:0000305}.
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DR   EMBL; D13419; BAA02684.1; -; Genomic_DNA.
DR   PIR; A47181; A47181.
DR   RefSeq; WP_042484030.1; NZ_CYTB01000001.1.
DR   AlphaFoldDB; P20960; -.
DR   SMR; P20960; -.
DR   STRING; 511.JT27_09845; -.
DR   KEGG; ag:BAA02684; -.
DR   eggNOG; COG0388; Bacteria.
DR   BioCyc; MetaCyc:MON-11587; -.
DR   BRENDA; 3.5.5.1; 232.
DR   GO; GO:0080061; F:indole-3-acetonitrile nitrilase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   CDD; cd07564; nitrilases_CHs; 1.
DR   Gene3D; 3.60.110.10; -; 1.
DR   InterPro; IPR003010; C-N_Hydrolase.
DR   InterPro; IPR036526; C-N_Hydrolase_sf.
DR   InterPro; IPR000132; Nitrilase/CN_hydratase_CS.
DR   InterPro; IPR044149; Nitrilases_CHs.
DR   PANTHER; PTHR46044; PTHR46044; 1.
DR   Pfam; PF00795; CN_hydrolase; 1.
DR   SUPFAM; SSF56317; SSF56317; 1.
DR   PROSITE; PS50263; CN_HYDROLASE; 1.
DR   PROSITE; PS00920; NITRIL_CHT_1; 1.
DR   PROSITE; PS00921; NITRIL_CHT_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase.
FT   CHAIN           1..356
FT                   /note="Nitrilase, arylacetone-specific"
FT                   /id="PRO_0000204042"
FT   DOMAIN          7..280
FT                   /note="CN hydrolase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   REGION          324..356
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        336..356
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        47
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        129
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        163
FT                   /note="Nucleophile"
FT   MUTAGEN         162
FT                   /note="C->N: Increase of activity."
FT                   /evidence="ECO:0000269|PubMed:8419930"
FT   MUTAGEN         163
FT                   /note="C->A: 100% loss of activity."
FT                   /evidence="ECO:0000269|PubMed:8419930"
SQ   SEQUENCE   356 AA;  38908 MW;  2195465A38D40FF8 CRC64;
     MQTRKIVRAA AVQAASPNYD LATGVDKTIE LARQARDEGC DLIVFGETWL PGYPFHVWLG
     APAWSLKYSA RYYANSLSLD SAEFQRIAQA ARTLGIFIAL GYSERSGGSL YLGQCLIDDK
     GQMLWSRRKL KPTHVERTVF GEGYARDLIV SDTELGRVGA LCCWEHLSPL SKYALYSQHE
     AIHIAAWPSF SLYSEQAHAL SAKVNMAASQ IYSVEGQCFT IAASSVVTQE TLDMLEVGEH
     NASLLKVGGG SSMIFAPDGR TLAPYLPHDA EGLIIADLNM EEIAFAKAIN DPVGHYSKPE
     ATRLVLDLGH REPMTRVHSK SVIQEEAPEP HVQSTAAPVA VSQTQDSDTL LVQEPS