NRM1_YEAST
ID NRM1_YEAST Reviewed; 249 AA.
AC P53718; D6W1I4;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Transcription factor NRM1;
DE AltName: Full=Negative regulator of MBF targets 1;
GN Name=NRM1; OrderedLocusNames=YNR009W; ORFNames=N2044;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=7900425; DOI=10.1002/yea.320101013;
RA Verhasselt P., Aert R., Voet M., Volckaert G.;
RT "Twelve open reading frames revealed in the 23.6 kb segment flanking the
RT centromere on the Saccharomyces cerevisiae chromosome XIV right arm.";
RL Yeast 10:1355-1361(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP IDENTIFICATION IN THE MBF COMPLEX, FUNCTION, AND INDUCTION.
RX PubMed=16916637; DOI=10.1016/j.molcel.2006.06.025;
RA de Bruin R.A.M., Kalashnikova T.I., Chahwan C., McDonald W.H.,
RA Wohlschlegel J., Yates J. III, Russell P., Wittenberg C.;
RT "Constraining G1-specific transcription to late G1 phase: the MBF-
RT associated corepressor Nrm1 acts via negative feedback.";
RL Mol. Cell 23:483-496(2006).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT "Analysis of phosphorylation sites on proteins from Saccharomyces
RT cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-145, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- FUNCTION: Negative regulatory component of the MBF complex involved in
CC cell-cycle-dependent transcription. {ECO:0000269|PubMed:16916637}.
CC -!- SUBUNIT: The MBF complex is composed of at least SWI6, MBP1 and NRM1.
CC {ECO:0000269|PubMed:16916637}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000269|PubMed:14562095}.
CC -!- INDUCTION: During G1 phase of the cell cycle.
CC {ECO:0000269|PubMed:16916637}.
CC -!- MISCELLANEOUS: Present with 2880 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the WHI5/NRM1 family. {ECO:0000305}.
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DR EMBL; X77395; CAA54577.1; -; Genomic_DNA.
DR EMBL; Z71624; CAA96286.1; -; Genomic_DNA.
DR EMBL; AY692622; AAT92641.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10550.1; -; Genomic_DNA.
DR PIR; S48346; S48346.
DR RefSeq; NP_014406.1; NM_001183186.1.
DR AlphaFoldDB; P53718; -.
DR SMR; P53718; -.
DR BioGRID; 35834; 62.
DR DIP; DIP-4530N; -.
DR IntAct; P53718; 2.
DR STRING; 4932.YNR009W; -.
DR iPTMnet; P53718; -.
DR MaxQB; P53718; -.
DR PaxDb; P53718; -.
DR PRIDE; P53718; -.
DR EnsemblFungi; YNR009W_mRNA; YNR009W; YNR009W.
DR GeneID; 855743; -.
DR KEGG; sce:YNR009W; -.
DR SGD; S000005292; NRM1.
DR VEuPathDB; FungiDB:YNR009W; -.
DR eggNOG; ENOG502S72A; Eukaryota.
DR HOGENOM; CLU_098759_0_0_1; -.
DR InParanoid; P53718; -.
DR OMA; QFAYYKY; -.
DR BioCyc; YEAST:G3O-33326-MON; -.
DR PRO; PR:P53718; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P53718; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0030907; C:MBF transcription complex; IDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0140297; F:DNA-binding transcription factor binding; IPI:SGD.
DR GO; GO:0071930; P:negative regulation of transcription involved in G1/S transition of mitotic cell cycle; IMP:SGD.
DR InterPro; IPR013734; TF_Nrm1/Whi5.
DR Pfam; PF08528; Whi5; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Phosphoprotein; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..249
FT /note="Transcription factor NRM1"
FT /id="PRO_0000203470"
FT REGION 208..230
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 145
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
SQ SEQUENCE 249 AA; 27564 MW; F776CC26B92C8C46 CRC64;
MSIMKQRLPL GEFSSSKINK LAIANIADAS EPRNHGENNV GTVCLPSIKS LMVSPEVYEN
TKSLPVPLMR SSGGGMACAS KSSCQDGIST KTTSRDYSEL SKKLQIRLQF AYYKYKTKQT
DKNFTDLKSK HSITRPSKVA THSKSEPLTR RRKLVLSQGH YKTPARSKIK TPSSICSHDN
TSSFTSFRGV SESSSTTADM NVADTTTPIR NNINTKHSNS HNRTLYQRQE TPTSIKAAKS
LIHLFTSNQ
