NRNA_MYCGE
ID NRNA_MYCGE Reviewed; 318 AA.
AC P22746;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 31-JAN-2018, sequence version 4.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Bifunctional oligoribonuclease and PAP phosphatase NrnA;
DE EC=3.1.-.-;
DE AltName: Full=3'(2'),5'-bisphosphate nucleotidase;
DE EC=3.1.3.7;
DE AltName: Full=3'-phosphoadenosine 5'-phosphate phosphatase;
DE Short=PAP phosphatase;
DE AltName: Full=Mgp-operon protein 1;
DE Short=Mgp1;
DE AltName: Full=ORF-1 protein;
DE AltName: Full=nanoRNase;
GN Name=nrnA; OrderedLocusNames=MG190;
OS Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
OS (Mycoplasmoides genitalium).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=243273;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT "The minimal gene complement of Mycoplasma genitalium.";
RL Science 270:397-403(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 66-318.
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=2583522; DOI=10.1016/0378-1119(89)90051-6;
RA Inamine J.M., Loechel S., Collier A.M., Barile M.F., Hu P.-C.;
RT "Nucleotide sequence of the MgPa (mgp) operon of Mycoplasma genitalium and
RT comparison to the P1 (mpp) operon of Mycoplasma pneumoniae.";
RL Gene 82:259-267(1989).
RN [3]
RP SEQUENCE REVISION TO 270, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=16407165; DOI=10.1073/pnas.0510013103;
RA Glass J.I., Assad-Garcia N., Alperovich N., Yooseph S., Lewis M.R.,
RA Maruf M., Hutchison C.A. III, Smith H.O., Venter J.C.;
RT "Essential genes of a minimal bacterium.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:425-430(2006).
CC -!- FUNCTION: Bifunctional enzyme which has both oligoribonuclease and pAp-
CC phosphatase activities. Degrades RNA and DNA oligonucleotides with a
CC length of 5 nucleotides and shorter, with a preference for longer
CC oligomers. Converts 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP
CC (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=adenosine 3',5'-bisphosphate + H2O = AMP + phosphate;
CC Xref=Rhea:RHEA:10040, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:58343, ChEBI:CHEBI:456215; EC=3.1.3.7;
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000250};
CC -!- DISRUPTION PHENOTYPE: Probably essential, it was not disrupted in a
CC global transposon mutagenesis study. {ECO:0000269|PubMed:16407165}.
CC -!- SIMILARITY: Belongs to the NrnA oligoribonuclease family.
CC {ECO:0000305}.
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DR EMBL; L43967; AAC71409.2; -; Genomic_DNA.
DR EMBL; M31431; AAA25419.1; -; Genomic_DNA.
DR PIR; A64221; A64221.
DR RefSeq; WP_009886005.1; NZ_AAGX01000020.1.
DR AlphaFoldDB; P22746; -.
DR SMR; P22746; -.
DR STRING; 243273.MG_190; -.
DR EnsemblBacteria; AAC71409; AAC71409; MG_190.
DR KEGG; mge:MG_190; -.
DR eggNOG; COG0618; Bacteria.
DR HOGENOM; CLU_039720_1_0_14; -.
DR OMA; CTRWVES; -.
DR OrthoDB; 1040743at2; -.
DR BioCyc; MGEN243273:G1GJ2-218-MON; -.
DR Proteomes; UP000000807; Chromosome.
DR GO; GO:0008441; F:3'(2'),5'-bisphosphate nucleotidase activity; IEA:UniProtKB-EC.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR InterPro; IPR001667; DDH_dom.
DR InterPro; IPR038763; DHH_sf.
DR InterPro; IPR003156; DHHA1_dom.
DR Pfam; PF01368; DHH; 1.
DR Pfam; PF02272; DHHA1; 1.
DR SUPFAM; SSF64182; SSF64182; 1.
PE 3: Inferred from homology;
KW Exonuclease; Hydrolase; Nuclease; Reference proteome.
FT CHAIN 1..318
FT /note="Bifunctional oligoribonuclease and PAP phosphatase
FT NrnA"
FT /id="PRO_0000096465"
SQ SEQUENCE 318 AA; 36413 MW; F48E4B51ABE55080 CRC64;
MKKGSITEAI NAIKQFDKIV IFHHVRPDGD CLGAQQGLFH LIKANFKNKE VKCVGNNNNL
FSFINMTFTN QIDESFLKEA LAIVVDANYK NRIELRELLD KNLFKAVLRI DHHPNEDDLN
TSFNFVEESY VACCEQIVEM ATVAKWTIPP VAATLLYIGI YTDSNRFLYS NTSYRTLYLA
AILYKAKADI RIVHDHLNHT SLADLKFKKY VYNHFKTQGQ VIYFICTKKI QKRLRMTADQ
CARVNLLSNI ADYKIWLFFI EQANNEIRIE LRSNGINVRD IAIKYGGGGH NNASGAIITN
KKQISDVVSD CVKKIVYN
