ID   NRNB_BACSU              Reviewed;         399 AA.
AC   O31824;
DT   15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Oligoribonuclease NrnB;
DE            EC=3.1.-.-;
DE   AltName: Full=NanoRNase B;
GN   Name=nrnB; Synonyms=yngD; OrderedLocusNames=BSU18200;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [2]
RP   FUNCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE,
RP   AND MUTAGENESIS OF 86-ASP--HIS-88.
RC   STRAIN=168;
RX   PubMed=19553197; DOI=10.1093/nar/gkp527;
RA   Fang M., Zeisberg W.-M., Condon C., Ogryzko V., Danchin A., Mechold U.;
RT   "Degradation of nanoRNA is performed by multiple redundant RNases in
RT   Bacillus subtilis.";
RL   Nucleic Acids Res. 37:5114-5125(2009).
CC   -!- FUNCTION: Degrades RNA oligonucleotides with a length of 5 nucleotides
CC       in a 3'- to 5'-direction. Less active on shorter RNA oligonucleotides
CC       and on those with a length of 24 nucleotides. Prefers RNA
CC       oligonucleotides containing adenines rather than cytosines.
CC       {ECO:0000269|PubMed:19553197}.
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000269|PubMed:19553197};
CC       Name=Co(2+); Xref=ChEBI:CHEBI:48828;
CC         Evidence={ECO:0000269|PubMed:19553197};
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000269|PubMed:19553197};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 7.5-8.0. {ECO:0000269|PubMed:19553197};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype. Cells show normal growth.
CC       {ECO:0000269|PubMed:19553197}.
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DR   EMBL; AL009126; CAB13703.1; -; Genomic_DNA.
DR   PIR; A69893; A69893.
DR   RefSeq; NP_389702.1; NC_000964.3.
DR   RefSeq; WP_003245472.1; NZ_JNCM01000035.1.
DR   AlphaFoldDB; O31824; -.
DR   SMR; O31824; -.
DR   IntAct; O31824; 2.
DR   STRING; 224308.BSU18200; -.
DR   PaxDb; O31824; -.
DR   PRIDE; O31824; -.
DR   EnsemblBacteria; CAB13703; CAB13703; BSU_18200.
DR   GeneID; 936020; -.
DR   KEGG; bsu:BSU18200; -.
DR   PATRIC; fig|224308.179.peg.1985; -.
DR   eggNOG; COG2404; Bacteria.
DR   InParanoid; O31824; -.
DR   OMA; YDTWEWE; -.
DR   BioCyc; BSUB:BSU18200-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR   InterPro; IPR038763; DHH_sf.
DR   SUPFAM; SSF64182; SSF64182; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Exonuclease; Hydrolase; Nuclease; Reference proteome.
FT   CHAIN           1..399
FT                   /note="Oligoribonuclease NrnB"
FT                   /id="PRO_0000390390"
FT   MUTAGEN         86..88
FT                   /note="DHH->AAA: Strong decrease in activity."
FT                   /evidence="ECO:0000269|PubMed:19553197"
SQ   SEQUENCE   399 AA;  46446 MW;  A6B72F5B85E2CB0D CRC64;
     MYHLYSHNDL DGVGCGIVAK LAFGKDVEIR YNSVNGLNAQ VQYFLEKAKE SNRQDALFIT
     DLAVNEENEE RLNEYVHAGG KVKLIDHHKT ALHLNEHEWG FVQVEYDDGR LTSATSLLYG
     YLIENGFMKP TNALDQFTEL VRQYDTWEWE RYDQKQAKRL NDLFFLLSID EFEAKMIQRL
     STHDEFFFDD FEEKLLDLED EKIERYLRRK KREMVQTFVH EHCVGIVHAE SYHSELGNRL
     GKDNPHLDYI AILSMGSKRV SLRTIHDYID VSEIAGRYGG GGHAKASGCS ITDEVYELFV
     AEAFRIDPVR PDAFRNIYNL KGSANGSLYE NRAQMRFFLF PLDNEWNIQI NGETQDETFA
     AFEEAEWFIK RNYAASLVRD EVFVAFLAEN LKLANQHRK