NRPA2_ARATH
ID NRPA2_ARATH Reviewed; 1178 AA.
AC F4I366; Q0WLL1; Q9C8S4; Q9FXG4;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=DNA-directed RNA polymerase I subunit 2 {ECO:0000305};
DE AltName: Full=DNA-directed RNA polymerase I subunit RPA2 {ECO:0000305};
DE Short=DNA polymerase I subunit A2 {ECO:0000305};
DE EC=2.7.7.6 {ECO:0000305};
DE AltName: Full=Nuclear RNA polymerase A2 {ECO:0000312|EMBL:AEE31152.1};
GN Name=NRPA2 {ECO:0000312|EMBL:AEE31152.1}; Synonyms=RPA2 {ECO:0000305};
GN OrderedLocusNames=At1g29940 {ECO:0000312|Araport:AT1G29940};
GN ORFNames=F1N18.2 {ECO:0000312|EMBL:AAG10602.1},
GN T1P2.15 {ECO:0000312|EMBL:AAG52049.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 601-1178.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18723889; DOI=10.1534/genetics.108.090621;
RA Onodera Y., Nakagawa K., Haag J.R., Pikaard D., Mikami T., Ream T., Ito Y.,
RA Pikaard C.S.;
RT "Sex-biased lethality or transmission of defective transcription machinery
RT in Arabidopsis.";
RL Genetics 180:207-218(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC Second largest core component of RNA polymerase I which synthesizes
CC ribosomal RNA precursors. Proposed to contribute to the polymerase
CC catalytic activity and forms the polymerase active center together with
CC the largest subunit. Pol I is composed of mobile elements and NRPA2 is
CC part of the core element with the central large cleft and probably a
CC clamp element that moves to open and close the cleft.
CC {ECO:0000250|UniProtKB:P22138}.
CC -!- FUNCTION: Essential for the completion of the three rounds of mitosis
CC in female megaspores required for the development of mature
CC gametophytes. {ECO:0000269|PubMed:18723889}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000305};
CC -!- SUBUNIT: Component of the RNA polymerase I (Pol I) complex consisting
CC of at least 13 subunits. {ECO:0000250|UniProtKB:P22138}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P22138}.
CC -!- DISRUPTION PHENOTYPE: Defect in seed production due to female
CC gametophyte developmental arrest. {ECO:0000269|PubMed:18723889}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG10602.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAG52049.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AY075644; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC008030; AAG10602.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC022455; AAG52049.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31152.1; -; Genomic_DNA.
DR EMBL; AY075644; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK230187; BAF01996.1; -; mRNA.
DR PIR; B86423; B86423.
DR RefSeq; NP_564341.2; NM_102734.5.
DR AlphaFoldDB; F4I366; -.
DR SMR; F4I366; -.
DR IntAct; F4I366; 1.
DR STRING; 3702.AT1G29940.1; -.
DR iPTMnet; F4I366; -.
DR PaxDb; F4I366; -.
DR PRIDE; F4I366; -.
DR ProteomicsDB; 250601; -.
DR EnsemblPlants; AT1G29940.1; AT1G29940.1; AT1G29940.
DR GeneID; 839872; -.
DR Gramene; AT1G29940.1; AT1G29940.1; AT1G29940.
DR KEGG; ath:AT1G29940; -.
DR Araport; AT1G29940; -.
DR TAIR; locus:2019272; AT1G29940.
DR eggNOG; KOG0216; Eukaryota.
DR HOGENOM; CLU_000524_5_1_1; -.
DR InParanoid; F4I366; -.
DR OMA; FFGVVHY; -.
DR OrthoDB; 42570at2759; -.
DR PRO; PR:F4I366; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I366; baseline and differential.
DR Genevisible; F4I366; AT.
DR GO; GO:0005736; C:RNA polymerase I complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0009561; P:megagametogenesis; IMP:UniProtKB.
DR GO; GO:0042254; P:ribosome biogenesis; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR InterPro; IPR009674; Rpa2_dom_4.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF06883; RNA_pol_Rpa2_4; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 2: Evidence at transcript level;
KW DNA-directed RNA polymerase; Metal-binding; Nucleotidyltransferase;
KW Nucleus; Reference proteome; Ribosome biogenesis; Transcription;
KW Transferase; Zinc; Zinc-finger.
FT CHAIN 1..1178
FT /note="DNA-directed RNA polymerase I subunit 2"
FT /id="PRO_0000434009"
FT ZN_FING 1097..1137
FT /note="C4-type"
FT /evidence="ECO:0000305"
FT CONFLICT 1002
FT /note="G -> D (in Ref. 4; BAF01996)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1178 AA; 132381 MW; 9660FCAEF8FD6086 CRC64;
MVVNAKDSTV PTMEDFKELH NLVTHHIESF DYMTLKGLDV MFNRIKPVSV YDPNTENELS
IWLENPLVFA PQKESFKSTS RKEPLLPFEC RQAKISYTGT FMADVCFKYN DGVVVRDKFD
FGQFPIMLMS KLCSLKGADC RKLLKCKEST SEMGGYFILN GIERVFRCVI APKRNHPTSM
IRNSFRDRKE GYSSKAVVTR CVRDDQSSVT VKLYYLRNGS ARVGFWIVGR EYLLPVGLVL
KALTNSCDEE IYESLNCCYS EHYGRGDGAI GTQLVRERAK IILDEVRDLG LFTREQCRKH
LGQHFQPVLD GVKKESLSIV AEAVLRDYLF VHLDNDHDKF NLLIFIIQKL YSLVDQTSLP
DNPDSLQNQE ILVPGHVITI YLKEKLEEWL RKCKSLLKDE LDNTNSKFSF ESLADVKKLI
NKNPPRSIGT SIETLLKTGA LKTQSGLDLQ QRAGYTVQAE RLNFLRFLSF FRAVHRGASF
AGLRTTTVRK LLPESWGFLC PVHTPDGTPC GLLNHMTRTS RITSQFDSKG NIRDFLKIRK
SVVDVLTGAG MVPSLPKLVR AGPPKVIHVL LDGQVVGTLS SNLVTKVVSY IRRLKVEAPS
VIPEDLEVGY VPTSMGGSYP GLYLASCPAR FIRPVKNISI PSDNIELIGP FEQVFMEISC
PDGGNGGRNN SSLATHEEIH PTGMISVVAN LTPWSDHNQS PRNMYQCQMA KQTMAYSTQA
LQFRADQKIY HLQTPQSPVV RTKTYTTYSI DENPTGTNAI VAVLAHTGFD MEDAMILNKS
SVERGMCHGQ IYQTENIDLS DQNSRFDSGS KSFRRSTNKA EHFRIDADGL PSVGQKLYPD
EPYCSIYDEV TNKTRHMKRK GTDPVIVDFV SVDMKSKKHP QRANIRFRHA RNPIIGDKFS
SRHGQKGVCS QLWPDIDMPF NGVTGMRPDL IINPHAFPSR MTIAMLLESI AAKGGSLHGK
FVDATPFRDA VKKTNGEEES KSSLLVDDLG SMLKEKGFNH YGTETLYSGY LGVELKCEIF
MGPVYYQRLR HMVSDKFQVR STGQVDQLTH QPIKGRKRGG GIRFGEMERD SLLAHGASYL
LHDRLHTSSD HHIADVCSLC GSLLTSSVVN VQQKKLIQEI GKLPPGRTPK KVTCYSCKTS
KGMETVAMPY VFRYLAAELA SMNIKMTLQL SDREGVTD
