ID   NRPA_METMA              Reviewed;         134 AA.
AC   Q8PW88;
DT   07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Nif-regulating protein A {ECO:0000303|PubMed:24930989};
GN   Name=nrpA {ECO:0000303|PubMed:24930989};
GN   OrderedLocusNames=MM_1708 {ECO:0000312|EMBL:AAM31404.1};
OS   Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM
OS   11833 / OCM 88) (Methanosarcina frisia).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=192952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX   PubMed=12125824;
RA   Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA   Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA   Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA   Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA   Fritz H.-J., Gottschalk G.;
RT   "The genome of Methanosarcina mazei: evidence for lateral gene transfer
RT   between Bacteria and Archaea.";
RL   J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
RN   [2]
RP   FUNCTION, INTERACTION WITH TBPS, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX   PubMed=24930989; DOI=10.1111/febs.12876;
RA   Weidenbach K., Ehlers C., Schmitz R.A.;
RT   "The transcriptional activator NrpA is crucial for inducing nitrogen
RT   fixation in Methanosarcina mazei Goe1 under nitrogen-limited conditions.";
RL   FEBS J. 281:3507-3522(2014).
CC   -!- FUNCTION: Involved in nitrogen regulation. Enhances the transcription
CC       of the nitrogen fixation (nif) operon under nitrogen-limited
CC       conditions. Acts by binding to the nifH promoter region.
CC       {ECO:0000269|PubMed:24930989}.
CC   -!- SUBUNIT: Interacts with the general archaeal transcription factors
CC       TBPs. {ECO:0000269|PubMed:24930989}.
CC   -!- INDUCTION: Induced by nitrogen-limited conditions.
CC       {ECO:0000269|PubMed:24930989}.
CC   -!- DISRUPTION PHENOTYPE: Under nitrogen-limited conditions, mutant shows a
CC       considerably changed growth rate and a prolonged lag phase. Level of
CC       NifH is significantly decreased. {ECO:0000269|PubMed:24930989}.
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DR   EMBL; AE008384; AAM31404.1; -; Genomic_DNA.
DR   RefSeq; WP_011033650.1; NC_003901.1.
DR   AlphaFoldDB; Q8PW88; -.
DR   STRING; 192952.MM_1708; -.
DR   EnsemblBacteria; AAM31404; AAM31404; MM_1708.
DR   GeneID; 24840727; -.
DR   KEGG; mma:MM_1708; -.
DR   PATRIC; fig|192952.21.peg.1982; -.
DR   eggNOG; arCOG02680; Archaea.
DR   HOGENOM; CLU_1615313_0_0_2; -.
DR   OMA; TIWARPL; -.
DR   Proteomes; UP000000595; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR012041; Znf_CPxCG-like.
DR   PANTHER; PTHR42195; PTHR42195; 1.
DR   Pfam; PF19769; CPxCG_zf; 1.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Metal-binding; Reference proteome; Transcription;
KW   Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..134
FT                   /note="Nif-regulating protein A"
FT                   /id="PRO_0000431493"
FT   ZN_FING         3..36
FT                   /note="C4-type; atypical"
FT                   /evidence="ECO:0000303|PubMed:24930989"
SQ   SEQUENCE   134 AA;  15197 MW;  06383C3FB99397F1 CRC64;
     MHCLECGLVY IVSGLKVPEK ISVRVFVNRI EHPFTHWVKL NAGELLREDE EIILDKVKTG
     YGSPFVITSL QIGVRRVPSA LAEELDTIWA RPLNEITFAK RRKIRQKHPM SFENITSTAG
     TSTASEYLEV VDES