NRPA_SOYBN
ID NRPA_SOYBN Reviewed; 368 AA.
AC Q5JZR1; C6TKA1;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=DCD domain-containing protein NRP-A {ECO:0000305};
DE AltName: Full=Asparagine-rich protein {ECO:0000303|Ref.1};
DE Short=N-rich protein {ECO:0000303|Ref.1};
DE AltName: Full=N-rich protein A {ECO:0000303|PubMed:18490446};
GN Name=NRP-A {ECO:0000303|PubMed:18490446}; Synonyms=NRP {ECO:0000303|Ref.1};
GN ORFNames=GLYMA_20G066100 {ECO:0000312|EMBL:KRG90080.1};
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
RX DOI=10.1023/A:1011202225323;
RA Ludwig A.A., Tenhaken R.;
RT "A new cell wall located N-rich protein is strongly induced during the
RT hypersensitive response in Glycine max L.";
RL Eur. J. Plant Pathol. 107:323-336(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Cheung F., Xiao Y., Chan A., Moskal W., Town C.D.;
RL Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Williams 82;
RX PubMed=20075913; DOI=10.1038/nature08670;
RA Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA Stacey G., Shoemaker R.C., Jackson S.A.;
RT "Genome sequence of the palaeopolyploid soybean.";
RL Nature 463:178-183(2010).
RN [4]
RP INDUCTION.
RX PubMed=18036212; DOI=10.1186/1471-2164-8-431;
RA Irsigler A.S., Costa M.D., Zhang P., Reis P.A., Dewey R.E., Boston R.S.,
RA Fontes E.P.;
RT "Expression profiling on soybean leaves reveals integration of ER- and
RT osmotic-stress pathways.";
RL BMC Genomics 8:431-431(2007).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=18490446; DOI=10.1074/jbc.m802654200;
RA Costa M.D., Reis P.A., Valente M.A., Irsigler A.S., Carvalho C.M.,
RA Loureiro M.E., Aragao F.J., Boston R.S., Fietto L.G., Fontes E.P.;
RT "A new branch of endoplasmic reticulum stress signaling and the osmotic
RT signal converge on plant-specific asparagine-rich proteins to promote cell
RT death.";
RL J. Biol. Chem. 283:20209-20219(2008).
RN [6]
RP INDUCTION.
RX PubMed=21482825; DOI=10.1074/jbc.m111.233494;
RA Alves M.S., Reis P.A., Dadalto S.P., Faria J.A., Fontes E.P., Fietto L.G.;
RT "A novel transcription factor, ERD15 (Early Responsive to Dehydration 15),
RT connects endoplasmic reticulum stress with an osmotic stress-induced cell
RT death signal.";
RL J. Biol. Chem. 286:20020-20030(2011).
CC -!- FUNCTION: Involved in stress signaling pathway that mediates cell death
CC in response to endoplasmic reticulum (ER) stress and osmotic stress.
CC {ECO:0000269|PubMed:18490446}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18490446}.
CC -!- INDUCTION: Induced by infection with an avirulent strain of the
CC bacterial pathogen Pseudomonas syringae pv glycinea (Ref.1). Induced by
CC infection with the fungal pathogen Phytophtora sojae (Ref.1). Induced
CC by copper, cycloheximide and reduced glutathione (Ref.1). Induced by
CC tunicamycin, azetidine-2-carboxylate (AZC) and osmotic stress
CC (PubMed:18490446, PubMed:18036212). Induced by dithiothreitol (DTT)
CC (PubMed:18490446). Induced by wounding (PubMed:21482825).
CC {ECO:0000269|PubMed:18036212, ECO:0000269|PubMed:18490446,
CC ECO:0000269|PubMed:21482825, ECO:0000269|Ref.1}.
CC -!- MISCELLANEOUS: Overexpression of NRP in protoplasts induces caspase-3-
CC like activity and promotes extensive DNA fragmentation
CC (PubMed:18490446). Transient expression of NRP in planta causes leaf
CC yellowing, chlorophyll loss, malondialdehyde production, ethylene
CC accumulation, and induction of the senescence marker gene CP1
CC (PubMed:18490446). {ECO:0000269|PubMed:18490446}.
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DR EMBL; AJ875407; CAI44933.1; -; mRNA.
DR EMBL; BT098107; ACU23341.1; -; mRNA.
DR EMBL; CM000853; KRG90080.1; -; Genomic_DNA.
DR RefSeq; NP_001236375.1; NM_001249446.1.
DR AlphaFoldDB; Q5JZR1; -.
DR STRING; 3847.GLYMA20G16100.1; -.
DR PRIDE; Q5JZR1; -.
DR EnsemblPlants; KRG90080; KRG90080; GLYMA_20G066100.
DR GeneID; 547685; -.
DR Gramene; KRG90080; KRG90080; GLYMA_20G066100.
DR KEGG; gmx:547685; -.
DR eggNOG; ENOG502QSUM; Eukaryota.
DR HOGENOM; CLU_051751_0_0_1; -.
DR InParanoid; Q5JZR1; -.
DR OrthoDB; 1210304at2759; -.
DR Proteomes; UP000008827; Chromosome 20.
DR ExpressionAtlas; Q5JZR1; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0034976; P:response to endoplasmic reticulum stress; IMP:UniProtKB.
DR GO; GO:0009414; P:response to water deprivation; IMP:UniProtKB.
DR InterPro; IPR013989; Dev_and_cell_death_domain.
DR InterPro; IPR044832; NRP-like.
DR PANTHER; PTHR46034; PTHR46034; 1.
DR Pfam; PF10539; Dev_Cell_Death; 1.
DR SMART; SM00767; DCD; 1.
DR PROSITE; PS51222; DCD; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Reference proteome; Stress response.
FT CHAIN 1..368
FT /note="DCD domain-containing protein NRP-A"
FT /id="PRO_0000451106"
FT DOMAIN 223..355
FT /note="DCD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00569"
FT REGION 35..61
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 171..218
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 178..203
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 329
FT /note="L -> F (in Ref. 2; ACU23341)"
FT /evidence="ECO:0000305"
FT CONFLICT 348..349
FT /note="SL -> FF (in Ref. 2; ACU23341)"
FT /evidence="ECO:0000305"
FT CONFLICT 359
FT /note="F -> L (in Ref. 2; ACU23341)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 368 AA; 41550 MW; B972367270CBE48A CRC64;
MDNNNDFWKF SDQLRLESGL ANLSLNDYSI WSNSYSSKRP DQRRNFDVKG SDFNNNNNSS
KAFDDDFNDG WKITNSNGPL FSMPHNNNNN TLEVGGFNKG GGIYSNTNTT ISSYHPNNLN
NNAFGGFNKG IYSNTTSSPY LNNNHHHLDD NNNLNRNNLK GYKTYFKGED QFHTPKSAKK
KNTTNNTNNK KHGDNTNNND GTKTGAEKKF KTLPPSESLP KNETIGGYIF VCNNDTMAEN
LQRQLFGLPP RYRDSVRTIT PGLPIFLYNY STHQLHGIFE AASFGGSNID PTAWEDKKCP
GESRFPAQVQ VITRKVCEPL EEDSFRPILH HYDGPKFRLE LSVPEALSLL DIFANQNSFD
DIFKAIPA
