NRPB7_ARATH
ID NRPB7_ARATH Reviewed; 176 AA.
AC P38421; Q541V8;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=DNA-directed RNA polymerase II subunit 7;
DE Short=RNA polymerase II subunit B7;
GN Name=NRPB7; Synonyms=RPB19; OrderedLocusNames=At5g59180; ORFNames=MNC17.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC STRAIN=cv. Columbia;
RX PubMed=1429663; DOI=10.1016/s0021-9258(18)50071-5;
RA Ulmasov T.N., Guilfoyle T.J.;
RT "Sequence of the fifth largest subunit of RNA polymerase II from plants.";
RL J. Biol. Chem. 267:23165-23169(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT features of the regions of 1,081,958 bp covered by seventeen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:379-391(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP SUBUNIT, AND INTERACTION WITH NRPB4.
RX PubMed=9488692; DOI=10.1074/jbc.273.10.5631;
RA Larkin R.M., Guilfoyle T.J.;
RT "Two small subunits in Arabidopsis RNA polymerase II are related to yeast
RT RPB4 and RPB7 and interact with one another.";
RL J. Biol. Chem. 273:5631-5637(1998).
RN [7]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND NOMENCLATURE.
RX PubMed=19110459; DOI=10.1016/j.molcel.2008.12.015;
RA Ream T.S., Haag J.R., Wierzbicki A.T., Nicora C.D., Norbeck A.D., Zhu J.K.,
RA Hagen G., Guilfoyle T.J., Pasa-Tolic L., Pikaard C.S.;
RT "Subunit compositions of the RNA-silencing enzymes Pol IV and Pol V reveal
RT their origins as specialized forms of RNA polymerase II.";
RL Mol. Cell 33:192-203(2009).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC Component of RNA polymerase II which synthesizes mRNA precursors and
CC many functional non-coding RNAs. Pol II is the central component of the
CC basal RNA polymerase II transcription machinery. It is composed of
CC mobile elements that move relative to each other. NRPB7 is part of a
CC subcomplex with NRPB4 that binds to a pocket formed by NRPB1, NRPB2 and
CC NRPB6 at the base of the clamp element. The NRBP4-NRPB7 subcomplex
CC seems to lock the clamp via NRPB7 in the closed conformation thus
CC preventing double-stranded DNA to enter the active site cleft. The
CC NRPB4-NRPB7 subcomplex binds single-stranded DNA and RNA (By
CC similarity). {ECO:0000250, ECO:0000269|PubMed:19110459}.
CC -!- SUBUNIT: Component of the RNA polymerase II complex consisting of at
CC least 12 subunits. Interacts with NRPB4. {ECO:0000269|PubMed:19110459,
CC ECO:0000269|PubMed:9488692}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit
CC family. {ECO:0000305}.
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DR EMBL; M90505; AAA32861.1; -; Genomic_DNA.
DR EMBL; AB016890; BAB09763.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97154.1; -; Genomic_DNA.
DR EMBL; AK118952; BAC43532.1; -; mRNA.
DR EMBL; BT005667; AAO64087.1; -; mRNA.
DR PIR; A44457; A44457.
DR RefSeq; NP_200726.1; NM_125308.4.
DR AlphaFoldDB; P38421; -.
DR SMR; P38421; -.
DR BioGRID; 21280; 19.
DR STRING; 3702.AT5G59180.1; -.
DR PaxDb; P38421; -.
DR PRIDE; P38421; -.
DR ProteomicsDB; 250567; -.
DR EnsemblPlants; AT5G59180.1; AT5G59180.1; AT5G59180.
DR GeneID; 836036; -.
DR Gramene; AT5G59180.1; AT5G59180.1; AT5G59180.
DR KEGG; ath:AT5G59180; -.
DR Araport; AT5G59180; -.
DR TAIR; locus:2168514; AT5G59180.
DR eggNOG; KOG3298; Eukaryota.
DR HOGENOM; CLU_085878_2_0_1; -.
DR InParanoid; P38421; -.
DR OMA; MRQPGLG; -.
DR OrthoDB; 1140388at2759; -.
DR PhylomeDB; P38421; -.
DR PRO; PR:P38421; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; P38421; baseline and differential.
DR Genevisible; P38421; AT.
DR GO; GO:0000932; C:P-body; IBA:GO_Central.
DR GO; GO:0005665; C:RNA polymerase II, core complex; IDA:UniProtKB.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0003727; F:single-stranded RNA binding; IBA:GO_Central.
DR GO; GO:0031369; F:translation initiation factor binding; IBA:GO_Central.
DR GO; GO:0000291; P:nuclear-transcribed mRNA catabolic process, exonucleolytic; IBA:GO_Central.
DR GO; GO:0060213; P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; IBA:GO_Central.
DR GO; GO:0045948; P:positive regulation of translational initiation; IBA:GO_Central.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.1490.120; -; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR036898; RNA_pol_Rpb7-like_N_sf.
DR InterPro; IPR045113; Rpb7-like.
DR InterPro; IPR005576; Rpb7-like_N.
DR InterPro; IPR003029; S1_domain.
DR PANTHER; PTHR12709; PTHR12709; 1.
DR Pfam; PF00575; S1; 1.
DR Pfam; PF03876; SHS2_Rpb7-N; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF88798; SSF88798; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; DNA-directed RNA polymerase; Nucleus;
KW Reference proteome; Transcription.
FT CHAIN 1..176
FT /note="DNA-directed RNA polymerase II subunit 7"
FT /id="PRO_0000073990"
SQ SEQUENCE 176 AA; 19462 MW; A508334EA3EA8186 CRC64;
MFFHIVLERN MQLHPRFFGR NLKENLVSKL MKDVEGTCSG RHGFVVAITG IDTIGKGLIR
DGTGFVTFPV KYQCVVFRPF KGEILEAVVT LVNKMGFFAE AGPVQIFVSK HLIPDDMEFQ
AGDMPNYTTS DGSVKIQKEC EVRLKIIGTR VDATAIFCVG TIKDDFLGVI NDPAAA
