ID   NRPR2_METMA             Reviewed;         252 AA.
AC   Q8PVJ4;
DT   07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Global nitrogen regulator NrpRII {ECO:0000305};
DE   AltName: Full=Nitrogen regulatory protein R II {ECO:0000305};
GN   Name=nrpRII {ECO:0000303|PubMed:18415079};
GN   OrderedLocusNames=MM_1969 {ECO:0000312|EMBL:AAM31665.1};
OS   Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM
OS   11833 / OCM 88) (Methanosarcina frisia).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=192952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX   PubMed=12125824;
RA   Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA   Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA   Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA   Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA   Fritz H.-J., Gottschalk G.;
RT   "The genome of Methanosarcina mazei: evidence for lateral gene transfer
RT   between Bacteria and Archaea.";
RL   J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
RN   [2]
RP   GENE NAME.
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX   PubMed=18415079; DOI=10.1007/s00203-008-0369-3;
RA   Weidenbach K., Ehlers C., Kock J., Ehrenreich A., Schmitz R.A.;
RT   "Insights into the NrpR regulon in Methanosarcina mazei Goe1.";
RL   Arch. Microbiol. 190:319-332(2008).
RN   [3]
RP   FUNCTION, ACTIVITY REGULATION, SUBUNIT, AND INTERACTION WITH NRPRI; TBP AND
RP   TFB.
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX   PubMed=20875081; DOI=10.1111/j.1742-4658.2010.07821.x;
RA   Weidenbach K., Ehlers C., Kock J., Schmitz R.A.;
RT   "NrpRII mediates contacts between NrpRI and general transcription factors
RT   in the archaeon Methanosarcina mazei Goe1.";
RL   FEBS J. 277:4398-4411(2010).
CC   -!- FUNCTION: Involved in nitrogen regulation. Under nitrogen sufficiency,
CC       represses the transcription of the nifH and the glnk1 promoters, via
CC       formation of a complex with the global nitrogen regulator NrpRI. Does
CC       not bind DNA directly. {ECO:0000269|PubMed:20875081}.
CC   -!- ACTIVITY REGULATION: Under nitrogen limitation, binding of 2-
CC       oxoglutarate to the NrpRI/NrpRII complex decreases the binding affinity
CC       of NrpRI to DNA as well as the binding affinity of NrpRII to TBP and
CC       TFB, which leads to removal of the complex from the operator, RNA
CC       polymerase recruitment and initiation of transcription.
CC       {ECO:0000269|PubMed:20875081}.
CC   -!- SUBUNIT: Forms a complex with NrpRI and the general archaeal
CC       transcription factors TBP and TFB. Interacts directly with NrpRI, TBP
CC       and TFB. {ECO:0000269|PubMed:20875081}.
CC   -!- INTERACTION:
CC       Q8PVJ4; Q8PXY1: nrpRI; NbExp=3; IntAct=EBI-8377070, EBI-8377032;
CC   -!- SIMILARITY: Belongs to the NrpR family. {ECO:0000305}.
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DR   EMBL; AE008384; AAM31665.1; -; Genomic_DNA.
DR   RefSeq; WP_011033901.1; NC_003901.1.
DR   AlphaFoldDB; Q8PVJ4; -.
DR   SMR; Q8PVJ4; -.
DR   IntAct; Q8PVJ4; 5.
DR   MINT; Q8PVJ4; -.
DR   STRING; 192952.MM_1969; -.
DR   EnsemblBacteria; AAM31665; AAM31665; MM_1969.
DR   GeneID; 24878707; -.
DR   KEGG; mma:MM_1969; -.
DR   PATRIC; fig|192952.21.peg.2268; -.
DR   eggNOG; arCOG02711; Archaea.
DR   HOGENOM; CLU_073525_0_0_2; -.
DR   OMA; IGGTNPM; -.
DR   Proteomes; UP000000595; Chromosome.
DR   Gene3D; 3.30.70.1360; -; 2.
DR   InterPro; IPR002846; NRD.
DR   InterPro; IPR038982; NrpR.
DR   InterPro; IPR036984; NrpR_dom_sf.
DR   PANTHER; PTHR41964; PTHR41964; 1.
DR   Pfam; PF01995; NRD1_2; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..252
FT                   /note="Global nitrogen regulator NrpRII"
FT                   /id="PRO_0000431496"
FT   REGION          8..247
FT                   /note="NRD"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   252 AA;  27375 MW;  54DAFCC558762784 CRC64;
     MQKNGYRIQF TSSRIRDLMY RTTFDPKKMD GDIILNLSLI DKKDLDDVLG IFKMVISSGL
     SVTPYVKVIS EGESIGDMTI EKGKVGIGTV CSITIDGVLL KAGIPVNPKL GGVVQIRNGI
     PVRFTDVLTY VSTTVDPLEI LMSQGITSVS EMLRTGSGKV LANLREAPMV ARDEIESNLS
     DLLDAGFSGI LEVGEPNTRV LDVPIERDHL GIVVIGGTNP MAVVQEYGIP IDTSAMSRLI
     SFKEMSRIED LV