NRPS8_ASPFU
ID NRPS8_ASPFU Reviewed; 8515 AA.
AC Q4WVN4;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 23-FEB-2022, entry version 116.
DE RecName: Full=Nonribosomal peptide synthetase 8;
DE EC=6.3.2.-;
GN Name=NRPS8; Synonyms=pes3, pesI; ORFNames=AFUA_5G12730;
OS Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS A1100) (Aspergillus fumigatus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=330879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX PubMed=16372009; DOI=10.1038/nature04332;
RA Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA Barrell B.G., Denning D.W.;
RT "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT Aspergillus fumigatus.";
RL Nature 438:1151-1156(2005).
RN [2]
RP NOMENCLATURE.
RX PubMed=16962256; DOI=10.1016/j.gene.2006.07.008;
RA Cramer R.A. Jr., Stajich J.E., Yamanaka Y., Dietrich F.S., Steinbach W.J.,
RA Perfect J.R.;
RT "Phylogenomic analysis of non-ribosomal peptide synthetases in the genus
RT Aspergillus.";
RL Gene 383:24-32(2006).
RN [3]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=21746855; DOI=10.1128/iai.00192-11;
RA O'Hanlon K.A., Cairns T., Stack D., Schrettl M., Bignell E.M., Kavanagh K.,
RA Miggin S.M., O'Keeffe G., Larsen T.O., Doyle S.;
RT "Targeted disruption of nonribosomal peptide synthetase pes3 augments the
RT virulence of Aspergillus fumigatus.";
RL Infect. Immun. 79:3978-3992(2011).
RN [4]
RP REVIEW ON FUNCTION, AND DOMAIN.
RX PubMed=17464044; DOI=10.1099/mic.0.2006/006908-0;
RA Stack D., Neville C., Doyle S.;
RT "Nonribosomal peptide synthesis in Aspergillus fumigatus and other fungi.";
RL Microbiology 153:1297-1306(2007).
CC -!- FUNCTION: Nonribosomal peptide synthesis (NRPS) is a key mechanism
CC responsible for the biosynthesis of bioactive metabolites which are
CC potentially contributing to organismal virulence. However, contarary to
CC other nonribosomal peptide synthases, NRPS8 does not encode a secreted
CC peptide, but has more a structural role since it is involved in germ
CC tube formation. {ECO:0000269|PubMed:21746855}.
CC -!- DOMAIN: NRP synthetases are composed of discrete domains (adenylation
CC (A), thiolation (T) or peptidyl carrier protein (PCP) and condensation
CC (C) domains) which when grouped together are referred to as a single
CC module. Each module is responsible for the recognition (via the A
CC domain) and incorporation of a single amino acid into the growing
CC peptide product. Thus, an NRP synthetase is generally composed of one
CC or more modules and can terminate in a thioesterase domain (TE) that
CC releases the newly synthesized peptide from the enzyme. Occasionally,
CC epimerase (E) domains (responsible for l- to d- amino acid conversion)
CC are present within the NRP synthetase. NRPS8 has the following
CC architecture: A-T-C-A-T-E-C-A-T-C-T-E-C-A-T-C-A-T-C-A-T-E-C.
CC {ECO:0000269|PubMed:17464044}.
CC -!- DISRUPTION PHENOTYPE: In contrast to other NRP synthetases, deletion of
CC NRPS8 significantly increases the virulence, probably through aberrant
CC innate immune recognition of the fungus. {ECO:0000269|PubMed:21746855}.
CC -!- SIMILARITY: Belongs to the NRP synthetase family. {ECO:0000305}.
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DR EMBL; AAHF01000003; EAL91342.1; -; Genomic_DNA.
DR RefSeq; XP_753380.1; XM_748287.1.
DR SMR; Q4WVN4; -.
DR STRING; 746128.CADAFUBP00005903; -.
DR PRIDE; Q4WVN4; -.
DR EnsemblFungi; EAL91342; EAL91342; AFUA_5G12730.
DR GeneID; 3511687; -.
DR KEGG; afm:AFUA_5G12730; -.
DR VEuPathDB; FungiDB:Afu5g12730; -.
DR eggNOG; KOG1178; Eukaryota.
DR HOGENOM; CLU_222660_0_0_1; -.
DR InParanoid; Q4WVN4; -.
DR OMA; QYIEWLE; -.
DR OrthoDB; 4243at2759; -.
DR Proteomes; UP000002530; Chromosome 5.
DR GO; GO:0031956; F:medium-chain fatty acid-CoA ligase activity; IBA:GO_Central.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006631; P:fatty acid metabolic process; IBA:GO_Central.
DR GO; GO:0019184; P:nonribosomal peptide biosynthetic process; ISM:AspGD.
DR GO; GO:0019748; P:secondary metabolic process; NAS:AspGD.
DR GO; GO:0009847; P:spore germination; IMP:AspGD.
DR Gene3D; 1.10.1200.10; -; 7.
DR Gene3D; 3.30.300.30; -; 6.
DR Gene3D; 3.30.559.10; -; 10.
DR Gene3D; 3.40.50.12780; -; 5.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR Pfam; PF00501; AMP-binding; 6.
DR Pfam; PF00668; Condensation; 10.
DR Pfam; PF00550; PP-binding; 7.
DR SMART; SM00823; PKS_PP; 7.
DR SUPFAM; SSF47336; SSF47336; 7.
DR TIGRFAMs; TIGR01733; AA-adenyl-dom; 6.
DR PROSITE; PS00455; AMP_BINDING; 6.
DR PROSITE; PS50075; CARRIER; 7.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
DR PROSITE; PS50007; PIPLC_X_DOMAIN; 1.
PE 3: Inferred from homology;
KW Ligase; Phosphopantetheine; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..8515
FT /note="Nonribosomal peptide synthetase 8"
FT /id="PRO_0000416549"
FT DOMAIN 615..691
FT /note="Carrier 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 1732..1808
FT /note="Carrier 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 3286..3362
FT /note="Carrier 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 3857..3933
FT /note="Carrier 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 5385..5461
FT /note="Carrier 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 6482..6558
FT /note="Carrier 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 7575..7651
FT /note="Carrier 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT REGION 59..736
FT /note="Adenylation 1"
FT REGION 587..1159
FT /note="Condensation 1"
FT REGION 1163..1705
FT /note="Adenylation 2"
FT REGION 1830..2273
FT /note="Epimerase 1"
FT REGION 2301..2709
FT /note="Condensation 2"
FT REGION 2733..3266
FT /note="Adenylation 3"
FT REGION 3406..3819
FT /note="Condensation 3"
FT REGION 3953..4392
FT /note="Epimerase 2"
FT REGION 4420..4823
FT /note="Condensation 4"
FT REGION 4837..5363
FT /note="Adenylation 4"
FT REGION 5508..5923
FT /note="Condensation 5"
FT REGION 5941..6459
FT /note="Adenylation 5"
FT REGION 6606..6992
FT /note="Condensation 6"
FT REGION 7030..7544
FT /note="Adenylation 6"
FT REGION 7670..8119
FT /note="Epimerase 3"
FT REGION 8164..8504
FT /note="Condensation 7"
FT REGION 8488..8515
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 652
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 1769
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 3323
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 3894
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 5422
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 6519
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 7612
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ SEQUENCE 8515 AA; 940213 MW; 3488DFADACA264D0 CRC64;
MSNNTMVNMS IEMKYDNDDT TSPDQKLPGL EALEMVKGAA SVVAEIEPSS SASSIMGSRE
HHERMLGPLE VCVHDLVQEQ SRQHGQTIAV HSIEKDLTYA ELERYADWMA KYLMVKRDIQ
AGHIIPFCLK KSVWTMVAML AIMKTGAACA ALDPSQPVSR IKRILDDTEA PLVIVHRDYL
GLAETLDVPS IVLGPDLWEG TSSGDTDKPL PVVDATQPAY IAFTSGSTGE PKGIVVPHRS
IATSMREHGP ATRVDTETRA LQFASYTFDM SFQEMFTTLT HGGCVCVPSE AERWNDLAGA
MERLGVNWAK LTPTVVRLLH PEQVPSLRTL VVGGEPITQD IIQTWAHRVD LIVSYGPAEA
SIMAAVSDPL APTALPRVIG RQVGGTLHVV DAGNHDRLVE GSGEGELLIE GPILATGYLK
DQSRTDATFI IDPKWVSCDS ENVTTAPRRF LKTGDLVHRD EDGVLYHLGR KDFRLKLNGR
LIDLAAIETC LLGSAQIASA VVIATREDTF WQQSLVAVIS AKPTPSSRPD PHDAGHIRIQ
TQHDLMLPLI RDLKDVAQSA LPDYMVPTVW LVVDSVPRLP SGKIHRRRVV QWLENLDEFT
VQEIRSMQNH TPHCEPETAM ERRLRDIWAE VLNMPVSTVS TMASFTGLGG DSVAAMRLAR
RCAAQGLEID VQMILQNLSI TELAPRVKVA EHSTDTSADN ERFSIGISLD QLCQSLPPRL
RSSITLDDVV DTYRCSPMQE AILLSRSRFQ GYYDVRWVAE VVSIDQDTPI SLQRVRDAWV
DVVRRHPALR TFFVQDASTG NRLLTQVILG SFPPLVSIDH SQATLDGIMG VNAAESSDPL
HQEPHRLSLF SLPTGQVFLK LELNHALSDG VSTALIFRDL SLAYSKALPS SPAPSFGGFI
RRLNLGEAEA SSTALKYWTD RLTGMVPCLF PVLREAAWTG PSAVQHVEIP IHGSQGALRR
FCTQHGTTIA NVFQTAWALV LSIYTGTDDV SFCYLVSGRD ASVDNVDEIV GPLISIMVHR
LTLSRSLALL QVLRQVQSDF TVALGHQHCS LAQIAHSLNL RGQPMSNTVV NVQRRFSQGG
PDGVADVYIR GIDCCNPTEF AIAVDVEDWE TYMTARLSYW ESCISQTQAE GIAETLAEVI
RNIQTNPLQT VGEVALVGDA VLAKLSTWNA VLPEANEACL PELVERQVIS QPSAVAIDTD
AEQITYISLW NLSGLLAGRL IDSGVQPRDL VAVCVPQSSW AVIAMLAIQR AGGACVPLDP
KAPAQRWWEI ISRTGISTVV TSETKKHIMS SQLPGLQVVS ADGTEIDQHH LQGSGRVLPV
LSVDSTAYVL FTSGSTGSPK GIDVPHRAIC TSLCAHCPVL GITNETRSLQ FAAYTFDASI
EETFGVLVHG GCVCIPSEDT KMNGLVDFIN RKAISWAFFT PSLVRLIDPD LVPSLQTIVL
GGEAVGNDIF NTWSHRVDLI NGYGPAEASI CCAAAHLSLR QTQSPSTIGR AVGCRIWVVD
PQNINRLLPP DCVGELLIEG HIVANGYWGD EERTASSFLP PPEFLQSLSL EYPADNFRRC
FYRTGDLVRQ RHDGSLIYVG RSDWQTKVNG QRVEIGEVEA QLSFHMAKNH SNHLSMVCVP
KSGPWMKRLV AILSLDPEEC TVGNRSNVVF CLDKPETAAM IRTISKGIES SLPPFMIPTV
WIPVKQLPTL ASGKINRRCV QEWVETSNEG IFLSVSRINS EAFGTSSTET TRGLTPTETV
IRAIWSRVLN IPLHSIGLDN TFLSLGGDSI SAMQVVYQAH REGLTISVQD IFHCKTVAKL
GRHADHSSKN TSSSNLLAPP VDEVEVPFAL SPIQQWFFES VPHKPAILNH YNQSAHFRVM
KEIDHSQLFK ALQHVVQRHA MLRSRFILDS WSWQQKITTD IEGSFRLEVE SIDAITAFRS
CCERAQKMID IIHGPLLVAI LVKVAEPAQH FLVLIGHHLS MDVVSWSIIH RELEAFLAGG
QVIAPLSSTS FQQWARLHYE PNIVPAEPRE VLPFSVLEAD LDYWGMRDTA NEYRHGEYIT
TSVDEETTAS IFKEANIAIG TEPVELIMAA VLYSFGRIFH DRSLPALYME AHGREGDEFG
LDLSGTVGWF TTICPLQLHR ESLHTWARAV AQVKDRRRSI PAKGWAYFAC RTVSPKGQAS
FNHHQQMEIL FNFTGSTGDI NDEHDRFLSP VRLMEDSRSD FDPKTPRVAL FAIEASVENR
QLRFSVSYHR SMRHVPRVKQ WIHSLPSTLQ EGVQMLSTIG RQPTLYDCPL AALNYSDLDS
ILARIQQSQA DMVVEEIYPC SHIQEGILLS SMRNPGHYQV RWLVKVEARR GLPVSTQRLA
KAWQSVVRKH SILRTIFVDD PSGTSSFLQV VVEDPRYPAS IVEVQHRDSV ASLDEDIDFT
VGELPYRATI YQLHDGNVFF LLDISHAILD GTSMGILAHE LVRGYDGSLT GDEAPHYRDY
IRLLQTMPRN ETLAHWKAYL QDIEPCKMIS RNNCVEKVIT PEVRKVAVQL PSTESLQQFC
KTYEVTFANI LQAVWAVVLM HYSGSETVCF GYLSSGRDLP IPHVDRAVGP YINILPCAVR
LQQSSSRLDV VKAIQADLYQ NLAHEHCSLW QIHKELGLKG TTLFNTLVNF QKTTAAVEDA
SISLTTVASR DPSEYDLAFN VTDEGSSMTA ELAFWSSFMD EPDANDLSRA VSRVFNEFVR
SPEAVLHDLS PIGPLNVEQI VRLMPSPISG EQQCLHWLIE QWVCRTPDAP AVCSTELEWS
YAKLHQLTTS LSHHLCQLGV GRNDRAAICM EKSPWVIVAM LAVLQAGAAF VPLDPSHPRT
RRESMISSLD AQVLLISLDA DEDAHLTMSS CRQVRVGSTR GAGSDTGVSS NLPKNEPDDA
AYILFTSGST GQPKGVVVPH RAVCSSIKAW SDMLNIRSTT RSLQFAAYTF DAAIGEIFAV
LANGGCVCVP SESERLNFLP ETITQLDVNW SFLTPSVIRQ IDPSSVPTLQ TLALGGEPLS
KEVIETWCDR VHLINVYGPT ETCVFSHANP ITDSKQEPSL IGPPILGRSW VVSPFNIDIL
VPRGCIGELV IESPAVAAGY FNNPEQTAKA FIAPPRWWKL AQDALSGSNK RTEDELPRFY
LTGDMVRQNV DGSITYLGRR DTQTKINGQR VELGEIEYHI LRLPAVLHAF VGVPKGGPYE
SRLVAVVSLR SEDGSGRGEA VLPEDFRLEV VEPQIAARYT SDLIVHLERN LPAYMVPAFL
VVVRQLPLQP SGKINRRMIL SWLSEPTMAG LQKTQHNFTR REEEPTTLGP VEKQMREIWS
EVLNLPVTRI RLDQSFFDLG GDSITAMQVV SRCRRSGLQL TVQDLLRWKT ITKVTPRTIS
LSQVTGERVY SLTSAGSVVD MRSIASKLEA IGLPGRAGVK GVYACSPMQE GILLAALKSP
GKYEVVLMLE IRATGSQDRV DLELLESAWL QVVDRHDMLR TVFLQEKTAT GVFSQVVYKH
IDPPIEITTV DDLDTLRSAS FSAGHFDCIP YRVTLCKLSG SSLAYVRLDI SHAVVDGWSL
SILSRDLQQA YDGQLPSQPV AQYCELIQYL ESQPQETSME FWRHLLTGMV PCHLPNMISN
SGSHSTQEVK LHQTRLEVDR NQELRDFCAA HDITIANVFQ LAWAVVLYRY TGMEDVCFGY
LISGRDAPID NLEDAVGPFI NILVARATLR QGISVKEFLG EIRDTFLAMS AHQHTSLTQI
QHELAVGNLG LFNTALNVQH RALTQQNPHS DIEICELSGR DPSEFGAILN VIDAGNTLEF
ALSYWSDLLS EETGREICSF LSCILSAVLD NSGCSVKTVS QTAADAARSF IDRKGDGDIS
STPRGNPAPE CYRPLKEPTN LIALTVQQVC AEVLDLSVSS LSLDETFLSL GGDSLLAMKV
VSRCREHGVA LTVQHMLQNQ TIREVFEHAR FSDSFSYRQL RHTPDPTGIP FPLSPIQKLH
FHLMPTGQNY YNQSFFLRVT ERLEASAIER AVRLLVLRHS VLRARFNQQI DGAWAQVISP
DIEGSYHFSA TSLISWDDLW PLVEGAQKRL NIRQGPLLSV DVLNLQGGDQ HIYLVGHHLV
VDLVSWRIIL ADLEIILRGG ELSHDPPLSF QTWIRLATEY AQDNIDPATT LPFQLRPGNF
AYWGMAGIPN LAKDVSSLSF TLPTDITSSL LGPANASLGT EPTDLMIAAL AHTFSLVFHD
HSGLTIFQES HGREPWTPEI DLSATCGWFT VLTPLSVETD KVEFRNTLKR VKDLRRRIPG
KGWPYFASRF ASRRRTGQKL DEKDEIEVLF NFVGLYQQFN RNDSLFVRPV ADVSLPPDFS
PDSIRLALIE INSLVDGQGR MVMHVTYNSR MKRQEGLTAW LERSQQVLEE EMPKLLTAAP
ERTPSDFQLL SLSYNELSAL ESHCHKQFGL DLNAVEDIYP CSPMQEGILL SQVRDPSLYR
VEWVADVRCV GGEAVDLGQL KQAWGQVVRR HPILRTIFVE RDHDAGAYLQ LVLNQSLFPS
SCNSASAYKV LLHLPYHITF QCLPETDTVR ITLQANHAII DGVTLAILAR DLGSAYGGEL
PDVSGPPFSD FVKFLRTRTI DKDLQYWSEF LSDSQPTLFP SLGSQLGPTP GAEDDLFVEK
HIHFADGAKM HEFCATFGVT VLNLFQVAWA LVLRLYTGQD DVCFGYLASG RDSNVSGIDD
IAGPMINMLV CRLQPTRDKT PRELLKQAHK HLTLALSHQH VPLAEVQNRL RTHGTPLFNT
LVNLQKSNVG QGISHLTIST IGANDPNEFT IGLQIADNGQ SIDVLFGHWL SRVSVDQADL
LASLLSSTVN NIMARPRARL GTINFCNGVH AQKMAEWNEQ ARRPVVESTL HSIIQDQARQ
RPSTIAIAST EAMWTYEELE RAADQTARYL LRQGVQPGTI LPFCMAKSPR AIVVMLAILK
VGCACAALDP AHPPDRLKLI VQQTGAKFVI SEPVVMDSLI LDGTANILSL TDCGGSINEP
GLTPCQLPSV KPTDIAFIMF TSGSTGTPKG VLIQHDSICT SIQYNGEAEM VTSSTRGLQF
SSYAFDTSVD EIFTVLSRGG CVCVPTEAER MNHLAAFISR FDVNWLSITP TVARLIAPGE
VPSVRTIVLG GEEIDPGVVN HWKDHAELVA SYGPAEASIA CAASPVTSVV GDALLGRPVA
SSLWVVDPSD HDALMPIGTA GELVIGGPLV ARGYLNDPDR TSLAFVCPKW STELNLPFNR
FYRTGDMARW NVDGTLSYVG RLDTQVKLNG QRVELGEVER HLLAQPCLQC STCAVPQSGL
LANRLVGVIG LQTPQISAAD GFHCLEISQA RTLVPYASDA EESLRAKLPP YMVPTVWIGV
QSLPLNASGK LDRRKVNKWL ESFQDEDTLN IFQLVGSEQE AEDEPPLTPI QQTIRNIWAD
VLGRTSESIG LQRSFFALGG DSVAAIRVVA QCRQANLQLT VQDVFQARTI QSLAACATAI
IEKPVETVSS LLEPSQCENP ESELAKLDSE VLSGLGGPEN IEEIYPCSPM QEGILFSRSS
IGGSYDTRLV VEVLPRDGAE VDLDRLKNAW AAVVQRHPIL RTVFADRPSD DSAFIQVTFR
KYRPVIMGCE TSEQSLDDMI AMPVQPFDDR RNPPHRFTIC TSSQQRVFIL LEISHVLTDA
VSIDIIWRDL QLAYEGALTT SKAPRYSRFV SYLQGTSQKD HMAYWLKFLK DAEPCLFPHL
GTGNQKGATR AVSVTISRAM TDHIRQFCAS LQITVANLIQ VMWSMVLRSY TGMDDVSFGY
ITSGRDLPLD GIDDLVGPLI SMMISRVRYT PSMKVADVIK QVGQDTVASM AHQHCSLAAI
HREVGLKSRS LFNTVLTVVR PHSTQSIDSS LQLTQIASSA GTSEFDVVLE VSDSGVELDT
TLAYSESALR SEDATNLSQA IMCALNWIIA HPESLVDHLS LCSPDLISQM TAMNNASPEW
ELRQCLHELI SLRAHRQPDS PALWTGQGTM TYSELDSKST MLARQLISLG VRPGSLVPIC
LSKSTVAVLA MLAIMKAGGA FVPLDPLHPT QRLADLVQRT GAKLILSSAN TRNSAEFAGP
RVVEVEQLLS RVTSVNEIDG VCPAPDPEGI AYVLFTSGST GVPKGVVVPH RAVCCSIRAH
SEAMNINTTS RSLQFASYTF DACICEIFSV LVAGGTVCIP SEEERVHDLA GFITRSQANW
AFFTPTVIRT LGLSPSQVPS LRTLVLGGEV VTVHDARTWA GHVSLFNGYG PTETCVFCAT
TPIHPDGVTY GRIGRPIGCA AWVVRPDNHD ILLPPGCPGE LLIEGPIVSQ GYLNDPVRTQ
EAFITHPAWA QNRKLSQNQS SARRFYKTGD LVRQSPDGTL VYMARLDSQV KINGQRLDLG
EIRHQIHSVV SEDVQVFIDL LPPTCLPNEK ALLVAFLAST RFEPEQTSGF SPPIKALTSQ
LEQDLPRLLP RYMIPSVYLP LSAIPLTSGG KVDRQALRRR VSRMSMKELL VYTGEEQGTK
LPISTAEEQQ MQMLWAEVLR IPPETIGASD HFFRLGADSI DGMKLVALAQ RHGILITLAD
IFRSPRLSDL ATLLESPAHP DDSKHDLKSI IPAFSLLNVH SRNTVLKEIK ADYALDVSQI
EDIYPCTPLQ ESLMAASIQS HGAYVHHLVE KLPPSGEVPA IMSAWQSVIK MTPILRTRIV
QTVSAGLLQV VLKESVQWLH RRQAIQEYLD EDARHSMTLG DPLLRLACLH DPGTPHTGHI
VITIHHSIYD GWSLPHIRKL VYATQNGHPC STSLPFNRFI HYLERKSDSR ASDSFWQSFL
YRSQPLAFPP LPSTGYQPVG TDSVQLSVHW PSTFPPSAFT LSTFVRVAWA LVLGSYSGTD
DVIFGLSLSG RDTPIPGILD ILGPTICTVP FRVKFSGESI GALLERAHAD SAAMLPYQHI
GLHHIRQLGP DCQLACDFQT LLVIQPARDP SDPEPHSELT FTSSGGLTYA FALICQPHPS
GIELHGDFDS NCVSRPVAER LLSQMKSVMG TLIFGDRRKL AAEVDVIDIS QKATLATWQR
EPLQPGEGRV EDLIISRAQQ APDDLAIHAW DGELTYNELV EESATLAENL KRRGIGPGML
VPLCFVKSIY YVVTLLAVTR TGAAFVPIDP DAPIERMQKI LKLTNACCIL TSASLAEQTR
AKAPARVAVF AIPLDRSARM STDSDLMPGQ SIVSHEAVYV LFTSGSTGIP KGVVVTHSSM
KASLKAHGRR LGLSESSRVL QFSNHTFDVS LLEILTTLAY GGCVCIPSDG DRVNRLSEYM
RDAKVNFAIL TPSVARILSP VSVPDLRTLA LAGEAWGQEI VNIWRDSVRL FNAYGPTEAT
ILSAIGEVDA QCFRPNNIGS GSGALCWVTS PTDPTRLMAI GAAGELLLEG PILAQGYLGE
EEKTRAAFID PPMWRRELSS HGAPPCRSSL YRTGDLARYE EDGSITYLGR MDGQVKIRGQ
RTELSEIEHH ILASDAVRNA VVLLRKNKLV CVLSLQSTSL TPAPSRPGDI RPVSDDDRDA
ALRICLSIRA GLARKVPEYM VPDLWVPVID LPLSSSGKLA RKGVDDWLAS VDTKHLINLS
LQKIPLSTTS PSPNIASSSV ERAIRQVIAG ALQLPVQQVS VDAPFTALGG DSITAILVAS
KLRNMGILLT MRDILEFPSI QHLASREDLT APSPANLPLD VEQVNVPFEL TPIQRFFFCF
FPDGANHFNQ SILVRVARRF TYDQWITSLR ALVQRHGMLR ARFSNVDNNL QQRITDESEG
ACCVKWHTLE AMDPSYISTA LDRCERRIDI FQGPICAVEI FDFPHEQIFF IAAHHLVMDF
VSQQILLKDL DSLLAGEELS TPRPLSFQAW SLKQIEYGSN LALSPQAVLP HHENVPLANL
NYWGIAAMDS CYADSAVRVL EFDSTVTSSL VGDANRAFNS EPIELFIASL LHSFANTFTD
RSAPAIFKEG HGRQTREPRL DPSSTVGWFT TITPIALAVS PQLSTFEDTL RRVKDICRAI
PANGFDYFTS RFLNASGSSA FQSHGPMMEI VLNYAGVLNN VQQGGTLFCP IATEEQRQMR
YHDINPQLRR FAVFDIYAQV AGGKLSFTFA YSPSLNYQDR ISAWIESLRR LLEAISVDLP
AKQPQKTLAD YPRARLDYTA LERLHKDIIP SLYPAKLDDV WECSPTQTVM LRARSYQPLF
FSPHFIWKIA GTKASEGNRE RLTKAWKRIV ARHSVLRSVF TSQLTATYHQ IVLANPPFFI
TWADMAGKES PSEALRRLPP LPSDELHLAF RLTASEDDEG DLFCRLDINH ALIDHVSINV
ILSDVIAVYG GQPMDSDSAF STFRDYVEYS HLRLVDGEPY WQDRLHDACP CVLTQRPYRQ
IPGVLFSKSV SVNASALKAL CLSSGFTLAS LFQACWAVLL QRYVGSDDVL FGYIASNRGL
PIRGIDRMVG PLISILPRRV RLSPSASSVS EQVRAIAKHI HEQLHDDLEH HMSAGNTMAE
VIQRGRCIEE LLFPFDTAIN FRSQPSAAVN SVSSDPTSPL QFADGQDPMP VSHQP
