NRP_PINMG
ID NRP_PINMG Reviewed; 686 AA.
AC H2A0M0;
DT 13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2012, sequence version 1.
DT 03-AUG-2022, entry version 25.
DE RecName: Full=Asparagine-rich protein;
DE AltName: Full=Prism uncharacterized shell protein 1;
DE Short=PUSP1;
DE Flags: Precursor;
OS Margaritifera margaritifera (Freshwater pearl mussel).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Bivalvia;
OC Autobranchia; Pteriomorphia; Pterioida; Pterioidea; Pteriidae; Pinctada.
OX NCBI_TaxID=102329;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], AND IDENTIFICATION.
RC TISSUE=Mantle;
RX PubMed=21040589; DOI=10.1186/1471-2164-11-613;
RA Joubert C., Piquemal D., Marie B., Manchon L., Pierrat F.,
RA Zanella-Cleon I., Cochennec-Laureau N., Gueguen Y., Montagnani C.;
RT "Transcriptome and proteome analysis of Pinctada margaritifera calcifying
RT mantle and shell: focus on biomineralization.";
RL BMC Genomics 11:613-613(2010).
RN [2]
RP PROTEIN SEQUENCE OF 260-277; 595-616 AND 641-663, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RC TISSUE=Shell;
RX PubMed=23213212; DOI=10.1073/pnas.1210552109;
RA Marie B., Joubert C., Tayale A., Zanella-Cleon I., Belliard C.,
RA Piquemal D., Cochennec-Laureau N., Marin F., Gueguen Y., Montagnani C.;
RT "Different secretory repertoires control the biomineralization processes of
RT prism and nacre deposition of the pearl oyster shell.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:20986-20991(2012).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23213212}.
CC -!- TISSUE SPECIFICITY: Prismatic layer of shell (at protein level).
CC Expressed primarily in the mantle with highest level in the mantle edge
CC and lower level in the mantle pallium. {ECO:0000269|PubMed:23213212}.
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DR EMBL; HE610387; CCE46161.1; -; mRNA.
DR AlphaFoldDB; H2A0M0; -.
DR SMR; H2A0M0; -.
DR PRIDE; H2A0M0; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR SUPFAM; SSF49899; SSF49899; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Secreted; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..686
FT /note="Asparagine-rich protein"
FT /evidence="ECO:0000255"
FT /id="PRO_0000417943"
FT REGION 34..73
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 125..148
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 201..236
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..54
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 686 AA; 74849 MW; 685D68B9B33E90B0 CRC64;
MKGTSALLLI GFFHATISQD PGGTVVIGSL SGRKRGNLNT GGQITSNSAI LGDVNAGSKL
SEPPKRRNTD KTARMLENNP RIGGSLIPPP GVIMEPNPYD INPPYFVSNK NSQSTNSATN
TMLQSLTSDT KTTTRTSQTS STRASSSITQ GINTMNRNSM RFNQVDRNKI SGFVNSGGQI
QTNPLNTNSQ SSPILAAQRQ ITRQKSENTQ GNSIVRNGGT NSLNIPSSTR RSQPPNMAVQ
IGQNTATFNM GTDGKVLHKF LPTNLFENIN SVSKEPRNTA SVPGIGGMRN PGPSISIRNI
FGTNNIEGSS VQLTGSSGVF VSDPGPKGNP TDVPQFVPSG ISPTVRDPNA LDPFKSIRNQ
IVPDIKRNEV NRGNSMISAP VIDNPTNSNS MVELNSILQN VQNGFLSEAL GNSNNQNNRV
TNIVNQINSA DPQPVRRCQF LPYRDLRTNK IDRRYFRQLV NGKWLNLKCA DGAGFNETTC
LCSIHLTGDA QCSPEVRLNF NDGTIQNLTP INVHIDAEGV DASKGWAHFN GSTQMKFEYF
NAYDVQRDFL IKLRFKADSY IPNQSHPIVT NCVAGQENTD PSIGVFLTGN YPHKIVFILQ
TDKSKLLQHL IFDVPRDGWH DITYKYDGST LTGILDGKEK SLPTEGRIEN RQAVLVFGGC
GNRIFRGNID DIQIYTCIPP SHRNKG