NRR_ORYSJ
ID NRR_ORYSJ Reviewed; 127 AA.
AC Q5ZEF1;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Protein NEGATIVE REGULATOR OF RESISTANCE {ECO:0000303|PubMed:16115061};
DE AltName: Full=NPR1-interactor {ECO:0000305};
GN Name=NRR {ECO:0000303|PubMed:16115061};
GN OrderedLocusNames=Os01g0130200 {ECO:0000312|EMBL:BAS70208.1},
GN LOC_Os01g03940 {ECO:0000305};
GN ORFNames=P0408F06.32 {ECO:0000312|EMBL:BAD52486.1},
GN P0504H10.7 {ECO:0000312|EMBL:BAD52461.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP FUNCTION, INTERACTION WITH NPR1/NH1, SUBCELLULAR LOCATION, AND MUTAGENESIS
RP OF GLU-35; PHE-36 AND LEU-40.
RX PubMed=16115061; DOI=10.1111/j.1365-313x.2005.02485.x;
RA Chern M., Canlas P.E., Fitzgerald H.A., Ronald P.C.;
RT "Rice NRR, a negative regulator of disease resistance, interacts with
RT Arabidopsis NPR1 and rice NH1.";
RL Plant J. 43:623-635(2005).
RN [5]
RP FUNCTION, INTERACTION WITH NPR1/NH1, AND MUTAGENESIS OF TRP-62; PHE-66 AND
RP 107-ASP-LEU-108.
RX PubMed=22353606; DOI=10.1186/1746-4811-8-6;
RA Chern M., Bai W., Sze-To W.H., Canlas P.E., Bartley L.E., Ronald P.C.;
RT "A rice transient assay system identifies a novel domain in NRR required
RT for interaction with NH1/OsNPR1 and inhibition of NH1-mediated
RT transcriptional activation.";
RL Plant Methods 8:6-6(2012).
RN [6]
RP INTERACTION WITH NPR1/NH1 AND NPR3/NH3.
RX PubMed=24919709; DOI=10.1186/1471-2164-15-461;
RA Chern M., Bai W., Ruan D., Oh T., Chen X., Ronald P.C.;
RT "Interaction specificity and coexpression of rice NPR1 homologs 1 and 3
RT (NH1 and NH3), TGA transcription factors and negative regulator of
RT resistance (NRR) proteins.";
RL BMC Genomics 15:461-461(2014).
CC -!- FUNCTION: Acts as negative regulator of disease resistance. Acts on
CC basal resistance, age-related resistance and resistance mediated by the
CC LRR receptor kinase XA21. Plants over-expressing NRR display enhanced
CC susceptibility to the bacterial blight Xanthomonas oryzae pv. oryzae
CC (Xoo) (PubMed:16115061). Binds to and represses NPR1/NH1-mediated
CC transcriptional activation of LG2 in vitro (PubMed:22353606).
CC {ECO:0000269|PubMed:16115061, ECO:0000269|PubMed:22353606}.
CC -!- SUBUNIT: Interacts with NPR1/NH1 (PubMed:16115061, PubMed:22353606,
CC PubMed:24919709). Interacts with NPR3/NH3 (PubMed:24919709).
CC {ECO:0000269|PubMed:16115061, ECO:0000269|PubMed:22353606,
CC ECO:0000269|PubMed:24919709}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16115061}.
CC -!- SIMILARITY: Belongs to the NPR1-interactor family.
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DR EMBL; AP002526; BAD52461.1; -; Genomic_DNA.
DR EMBL; AP002538; BAD52486.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS70208.1; -; Genomic_DNA.
DR RefSeq; XP_015631290.1; XM_015775804.1.
DR AlphaFoldDB; Q5ZEF1; -.
DR SMR; Q5ZEF1; -.
DR STRING; 4530.OS01T0130200-00; -.
DR PaxDb; Q5ZEF1; -.
DR PRIDE; Q5ZEF1; -.
DR EnsemblPlants; Os01t0130200-00; Os01t0130200-00; Os01g0130200.
DR Gramene; Os01t0130200-00; Os01t0130200-00; Os01g0130200.
DR KEGG; osa:107278621; -.
DR eggNOG; ENOG502SWTX; Eukaryota.
DR HOGENOM; CLU_146278_0_0_1; -.
DR InParanoid; Q5ZEF1; -.
DR OMA; APPRALC; -.
DR OrthoDB; 1638543at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0042742; P:defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0010112; P:regulation of systemic acquired resistance; IEA:InterPro.
DR InterPro; IPR031425; NPR1/NH1-interacting.
DR PANTHER; PTHR33669; PTHR33669; 1.
PE 1: Evidence at protein level;
KW Nucleus; Plant defense; Reference proteome.
FT CHAIN 1..127
FT /note="Protein NEGATIVE REGULATOR OF RESISTANCE"
FT /id="PRO_0000437008"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 47..127
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 35
FT /note="E->K: Slightly reduces NPR1/NH1 binding efficiency."
FT /evidence="ECO:0000269|PubMed:16115061"
FT MUTAGEN 36
FT /note="F->G: Slightly reduces NPR1/NH1 binding efficiency."
FT /evidence="ECO:0000269|PubMed:16115061"
FT MUTAGEN 40
FT /note="L->G: Slightly reduces NPR1/NH1 binding efficiency."
FT /evidence="ECO:0000269|PubMed:16115061"
FT MUTAGEN 62
FT /note="W->A: Abolishes interaction with NPR1/NH1 and
FT repression of NPR1/NH1-mediated transcriptional activation
FT of LG2; when associated with A-66."
FT /evidence="ECO:0000269|PubMed:22353606"
FT MUTAGEN 66
FT /note="F->A: Abolishes interaction with NPR1/NH1 and
FT repression of NPR1/NH1-mediated transcriptional activation
FT of LG2;; when associated with A-62."
FT /evidence="ECO:0000269|PubMed:22353606"
FT MUTAGEN 107..108
FT /note="DL->AA: Increases NPR1/NH1 binding efficiency and
FT repression of NPR1/NH1-mediated transcriptional activation
FT of LG2."
FT /evidence="ECO:0000269|PubMed:22353606"
SQ SEQUENCE 127 AA; 13805 MW; 6A9A60D6FA7E24A8 CRC64;
MDATTTAKRK RPAASDIADD APTTVDEVSD AEVEEFYAIL RRMRDATRRL GARPPPPRAP
AWRPSFSWED FADAPPKQAP PPPQQPADHE RVAENATPPR RPAPGLDLNV EPPSDAPATP
RSARAPA
