ID   NRSF_AZOOP              Reviewed;         213 AA.
AC   G8QM60;
DT   05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT   22-FEB-2012, sequence version 1.
DT   03-AUG-2022, entry version 38.
DE   RecName: Full=Probable anti-sigma-F factor NrsF;
DE   AltName: Full=Negative regulator of sigma F {ECO:0000303|PubMed:25968643};
DE   AltName: Full=Regulator of SigF;
DE   AltName: Full=Sigma-F anti-sigma factor NrsF;
GN   Name=nrsf {ECO:0000303|PubMed:25968643}; OrderedLocusNames=Dsui_0154;
OS   Azospira oryzae (strain ATCC BAA-33 / DSM 13638 / PS) (Dechlorosoma
OS   suillum).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales;
OC   Rhodocyclaceae; Azospira.
OX   NCBI_TaxID=640081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-33 / DSM 13638 / PS;
RX   PubMed=22535943; DOI=10.1128/jb.00124-12;
RA   Byrne-Bailey K.G., Coates J.D.;
RT   "Complete genome sequence of the anaerobic perchlorate-reducing bacterium
RT   Azospira suillum strain PS.";
RL   J. Bacteriol. 194:2767-2768(2012).
RN   [2]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC BAA-33 / DSM 13638 / PS;
RX   PubMed=25968643; DOI=10.1128/mbio.00233-15;
RA   Melnyk R.A., Youngblut M.D., Clark I.C., Carlson H.K., Wetmore K.M.,
RA   Price M.N., Iavarone A.T., Deutschbauer A.M., Arkin A.P., Coates J.D.;
RT   "Novel mechanism for scavenging of hypochlorite involving a periplasmic
RT   methionine-rich peptide and methionine sulfoxide reductase.";
RL   MBio 6:E00233-E00233(2015).
CC   -!- FUNCTION: Probably an anti-sigma factor for extracytoplasmic function
CC       (ECF) sigma factor sigma-F (SigF), which responds to (hypo)chlorite.
CC       ECF sigma factors are held in an inactive form by a cognate anti-sigma
CC       factor (Probable). {ECO:0000269|PubMed:25968643,
CC       ECO:0000305|PubMed:25968643}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255}; Multi-pass
CC       membrane protein {ECO:0000255}.
CC   -!- INDUCTION: Part of the SigF regulon, induced by chlorite (HClO(2))
CC       under control of SigF. Part of the probable sigF-nrsF operon.
CC       {ECO:0000269|PubMed:25968643}.
CC   -!- DISRUPTION PHENOTYPE: Growth not inhibited by chlorite during aerobic
CC       growth, slight reduction in lag-phase when grown on minimal medium with
CC       lactate and chlorite. Up-regulation of the SigF regulon (Dsui_0156 to
CC       Dsui_0159). {ECO:0000269|PubMed:25968643}.
CC   -!- SIMILARITY: Belongs to the NrsF anti-sigma-F factor family.
CC       {ECO:0000305}.
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DR   EMBL; CP003153; AEV24576.1; -; Genomic_DNA.
DR   RefSeq; WP_014235278.1; NC_016616.1.
DR   AlphaFoldDB; G8QM60; -.
DR   STRING; 640081.Dsui_0154; -.
DR   EnsemblBacteria; AEV24576; AEV24576; Dsui_0154.
DR   KEGG; dsu:Dsui_0154; -.
DR   eggNOG; COG4944; Bacteria.
DR   HOGENOM; CLU_097826_1_0_4; -.
DR   OMA; FVLIWYT; -.
DR   OrthoDB; 1539086at2; -.
DR   Proteomes; UP000005633; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR009495; NrsF.
DR   Pfam; PF06532; NrsF; 1.
PE   2: Evidence at transcript level;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Stress response; Transcription; Transcription regulation; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..213
FT                   /note="Probable anti-sigma-F factor NrsF"
FT                   /id="PRO_0000440883"
FT   TRANSMEM        27..47
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        51..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        126..146
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        159..179
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        187..207
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   213 AA;  22450 MW;  FEA4242E24DF52D0 CRC64;
     MKTEDLITML AAGAGAVEAP SAAQRYALAI GWGAAGATLL MLALLQVRHD LGLALLLPMF
     WVKVGFVTCL AAGSLFAVLR LSRPGAKTNW VPAALGLPVL GMWAIAAFTL IEAEPMERSN
     LFFGDTWKSC PLLIAMLSVP VFAAVLRSMK DLAPTRPRLA GFAAGLLAGA VAAVVYCLHC
     PELGAPFIGF WYLLGMLIPA AVGVLLGNSM LRW