NRSF_CAUVN
ID NRSF_CAUVN Reviewed; 214 AA.
AC A0A0H3CC47;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 16-SEP-2015, sequence version 1.
DT 25-MAY-2022, entry version 21.
DE RecName: Full=Anti-sigma-F factor NrsF;
DE AltName: Full=Negative regulator of sigma F {ECO:0000303|PubMed:22985357};
DE AltName: Full=Regulator of SigF;
DE AltName: Full=Sigma-F anti-sigma factor NrsF;
GN Name=nrsF {ECO:0000303|PubMed:22985357}; OrderedLocusNames=CCNA_03273;
OS Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC Caulobacteraceae; Caulobacter.
OX NCBI_TaxID=565050;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NA1000 / CB15N;
RX PubMed=20472802; DOI=10.1128/jb.00255-10;
RA Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V.,
RA Walunas T.L., Crosson S.;
RT "The genetic basis of laboratory adaptation in Caulobacter crescentus.";
RL J. Bacteriol. 192:3678-3688(2010).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, INDUCTION, DISRUPTION PHENOTYPE, AND
RP MUTAGENESIS OF CYS-131 AND CYS-181.
RC STRAIN=NA1000 / CB15N;
RX PubMed=22985357; DOI=10.1186/1471-2180-12-210;
RA Kohler C., Lourenco R.F., Avelar G.M., Gomes S.L.;
RT "Extracytoplasmic function (ECF) sigma factor sigmaF is involved in
RT Caulobacter crescentus response to heavy metal stress.";
RL BMC Microbiol. 12:210-210(2012).
CC -!- FUNCTION: An anti-sigma factor for extracytoplasmic function (ECF)
CC sigma factor sigma-F (SigF), which responds to chromate and cadmium.
CC Overexpression leads to loss of response to dichromate. ECF sigma
CC factors are held in an inactive form by a cognate anti-sigma factor
CC (Probable).
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000305|PubMed:22985357}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- INDUCTION: Slightly induced by potassium dichromate (K(2)Cr(2)O(7))
CC (PubMed:22985357). Induction by Cr is partially dependent on sigF
CC (PubMed:22985357). Probably part of the sigF-nrsF operon
CC (PubMed:22985357). {ECO:0000269|PubMed:22985357}.
CC -!- DISRUPTION PHENOTYPE: Cannot be deleted in the presence of an intact
CC sigF gene, a double sigF-nrsF mutant is viable.
CC {ECO:0000269|PubMed:22985357}.
CC -!- SIMILARITY: Belongs to the NrsF anti-sigma-F factor family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP001340; ACL96826.1; -; Genomic_DNA.
DR RefSeq; WP_010921085.1; NC_011916.1.
DR RefSeq; YP_002518734.1; NC_011916.1.
DR AlphaFoldDB; A0A0H3CC47; -.
DR EnsemblBacteria; ACL96826; ACL96826; CCNA_03273.
DR GeneID; 7332035; -.
DR KEGG; ccs:CCNA_03273; -.
DR PATRIC; fig|565050.3.peg.3275; -.
DR HOGENOM; CLU_097826_1_0_5; -.
DR OMA; LYCAEMS; -.
DR OrthoDB; 1539086at2; -.
DR PhylomeDB; A0A0H3CC47; -.
DR Proteomes; UP000001364; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR009495; NrsF.
DR Pfam; PF06532; NrsF; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Stress response; Transcription; Transcription regulation; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..214
FT /note="Anti-sigma-F factor NrsF"
FT /id="PRO_0000440884"
FT TOPO_DOM 1..25
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 26..46
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 47..53
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 54..74
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 75..90
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 91..111
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..134
FT /note="Periplasmic"
FT /evidence="ECO:0000305|PubMed:22985357"
FT TRANSMEM 135..155
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 156..158
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 159..179
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 180..185
FT /note="Periplasmic"
FT /evidence="ECO:0000305|PubMed:22985357"
FT TRANSMEM 186..206
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 207..214
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT MUTAGEN 131
FT /note="C->S: Up-regulation of all sigF-regulon genes, even
FT in the absence of dichromate stress, increased expression
FT after stress. Greater up-regulation in the absence of
FT stress, some SigF protein mis-localized to the cytoplasm;
FT when associated with S-181."
FT /evidence="ECO:0000269|PubMed:22985357"
FT MUTAGEN 181
FT /note="C->S: Up-regulation of all sigF-regulon genes, even
FT in the absence of dichromate stress, increased expression
FT after stress. Greater up-regulation in the absence of
FT stress, some SigF protein mis-localized to the cytoplasm;
FT when associated with S-131."
FT /evidence="ECO:0000269|PubMed:22985357"
SQ SEQUENCE 214 AA; 22529 MW; A3E5AD21085FEBB2 CRC64;
MRTDDLIDAL AADAGRGTEP APPRRLALVA GLGGVAALLL VLGWLQARPD LGQAILGPMF
WVKAIYTGLL GLAGYLAVER LSRPGGSGRR GWIIGAVVFG ACAVAGIYQA ITSPDVQAAL
KLLHGYSWRS CSPRILVLGL PMLALGLWAL RGMAPTRPGL AGFAMGLFSG GVVATLYGLH
CPEHTFTFLA LWYSLGVLAL GLIGGWAGRW LLRW
