NRT21_ARATH
ID NRT21_ARATH Reviewed; 530 AA.
AC O82811;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=High-affinity nitrate transporter 2.1;
DE Short=AtNRT2:1;
DE AltName: Full=Protein ACH1;
DE AltName: Full=Protein LATERAL ROOT INITIATION 1;
GN Name=NRT2.1; Synonyms=ACH1, LIN1, NRT2;1AT; OrderedLocusNames=At1g08090;
GN ORFNames=T6D22.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Landsberg erecta;
RA Zhang H., Forde B.G.;
RT "Cloning and characterisation of the NRT2 high affinity nitrate
RT transporters from Arabidopsis.";
RL Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], INDUCTION BY NITRATE AND AMMONIUM, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=10205909; DOI=10.1046/j.1365-313x.1999.00396.x;
RA Zhuo D., Okamoto M., Vidmar J.J., Glass A.D.;
RT "Regulation of a putative high-affinity nitrate transporter (Nrt2;1At) in
RT roots of Arabidopsis thaliana.";
RL Plant J. 17:563-568(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=10066586; DOI=10.1016/s1369-5266(98)80110-6;
RA Daniel-Vedele F., Filleur S., Caboche M.;
RT "Nitrate transport: a key step in nitrate assimilation.";
RL Curr. Opin. Plant Biol. 1:235-239(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=9951738; DOI=10.1007/s004250050505;
RA Filleur S., Daniel-Vedele F.;
RT "Expression analysis of a high-affinity nitrate transporter isolated from
RT Arabidopsis thaliana by differential display.";
RL Planta 207:461-469(1999).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [7]
RP INDUCTION BY NITRATE AND LIGHT.
RX PubMed=10417701; DOI=10.1046/j.1365-313x.1999.00480.x;
RA Lejay L., Tillard P., Lepetit M., Olive F., Filleur S., Daniel-Vedele F.,
RA Gojon A.;
RT "Molecular and functional regulation of two NO3- uptake systems by N- and
RT C-status of Arabidopsis plants.";
RL Plant J. 18:509-519(1999).
RN [8]
RP DISRUPTION PHENOTYPE.
RX PubMed=11165253; DOI=10.1016/s0014-5793(01)02096-8;
RA Filleur S., Dorbe M.F., Cerezo M., Orsel M., Granier F., Gojon A.,
RA Daniel-Vedele F.;
RT "An arabidopsis T-DNA mutant affected in Nrt2 genes is impaired in nitrate
RT uptake.";
RL FEBS Lett. 489:220-224(2001).
RN [9]
RP FUNCTION.
RX PubMed=11553754; DOI=10.1104/pp.127.1.262;
RA Cerezo M., Tillard P., Filleur S., Munos S., Daniel-Vedele F., Gojon A.;
RT "Major alterations of the regulation of root NO(3)(-) uptake are associated
RT with the mutation of Nrt2.1 and Nrt2.2 genes in Arabidopsis.";
RL Plant Physiol. 127:262-271(2001).
RN [10]
RP GENE FAMILY.
RX PubMed=11912225; DOI=10.1093/jexbot/53.370.825;
RA Orsel M., Filleur S., Fraisier V., Daniel-Vedele F.;
RT "Nitrate transport in plants: which gene and which control?";
RL J. Exp. Bot. 53:825-833(2002).
RN [11]
RP INDUCTION BY NITRATE, AND GENE FAMILY.
RX PubMed=12668777; DOI=10.1093/pcp/pcg036;
RA Okamoto M., Vidmar J.J., Glass A.D.;
RT "Regulation of NRT1 and NRT2 gene families of Arabidopsis thaliana:
RT responses to nitrate provision.";
RL Plant Cell Physiol. 44:304-317(2003).
RN [12]
RP FUNCTION, AND INDUCTION BY NITRATE.
RX PubMed=15107992; DOI=10.1007/s00425-004-1266-x;
RA Orsel M., Eulenburg K., Krapp A., Daniel-Vedele F.;
RT "Disruption of the nitrate transporter genes AtNRT2.1 and AtNRT2.2
RT restricts growth at low external nitrate concentration.";
RL Planta 219:714-721(2004).
RN [13]
RP FUNCTION, MUTAGENESIS OF GLY-119, AND DISRUPTION PHENOTYPE.
RX PubMed=16157886; DOI=10.1073/pnas.0504219102;
RA Little D.Y., Rao H., Oliva S., Daniel-Vedele F., Krapp A., Malamy J.E.;
RT "The putative high-affinity nitrate transporter NRT2.1 represses lateral
RT root initiation in response to nutritional cues.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:13693-13698(2005).
RN [14]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=16415211; DOI=10.1104/pp.105.075721;
RA Remans T., Nacry P., Pervent M., Girin T., Tillard P., Lepetit M.,
RA Gojon A.;
RT "A central role for the nitrate transporter NRT2.1 in the integrated
RT morphological and physiological responses of the root system to nitrogen
RT limitation in Arabidopsis.";
RL Plant Physiol. 140:909-921(2006).
RN [15]
RP INDUCTION BY NITRATE.
RX PubMed=16415212; DOI=10.1104/pp.105.074385;
RA Okamoto M., Kumar A., Li W., Wang Y., Siddiqi M.Y., Crawford N.M.,
RA Glass A.D.;
RT "High-affinity nitrate transport in roots of Arabidopsis depends on
RT expression of the NAR2-like gene AtNRT3.1.";
RL Plant Physiol. 140:1036-1046(2006).
RN [16]
RP INTERACTION WITH NRT3.1, AND INDUCTION.
RX PubMed=17012411; DOI=10.1104/pp.106.085209;
RA Orsel M., Chopin F., Leleu O., Smith S.J., Krapp A., Daniel-Vedele F.,
RA Miller A.J.;
RT "Characterization of a two-component high-affinity nitrate uptake system in
RT Arabidopsis. Physiology and protein-protein interaction.";
RL Plant Physiol. 142:1304-1317(2006).
RN [17]
RP GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [18]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION BY LIGHT AND
RP SUCROSE.
RX PubMed=17573350; DOI=10.1074/jbc.m700901200;
RA Wirth J., Chopin F., Santoni V., Viennois G., Tillard P., Krapp A.,
RA Lejay L., Daniel-Vedele F., Gojon A.;
RT "Regulation of root nitrate uptake at the NRT2.1 protein level in
RT Arabidopsis thaliana.";
RL J. Biol. Chem. 282:23541-23552(2007).
RN [19]
RP TISSUE SPECIFICITY.
RX PubMed=17540716; DOI=10.1105/tpc.107.050542;
RA Chopin F., Orsel M., Dorbe M.F., Chardon F., Truong H.N., Miller A.J.,
RA Krapp A., Daniel-Vedele F.;
RT "The Arabidopsis ATNRT2.7 nitrate transporter controls nitrate content in
RT seeds.";
RL Plant Cell 19:1590-1602(2007).
RN [20]
RP INDUCTION BY NITRATE AND SUCROSE.
RX PubMed=17897408; DOI=10.1111/j.1365-3040.2007.01712.x;
RA Girin T., Lejay L., Wirth J., Widiez T., Palenchar P.M., Nazoa P.,
RA Touraine B., Gojon A., Lepetit M.;
RT "Identification of a 150 bp cis-acting element of the AtNRT2.1 promoter
RT involved in the regulation of gene expression by the N and C status of the
RT plant.";
RL Plant Cell Environ. 30:1366-1380(2007).
RN [21]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17085507; DOI=10.1104/pp.106.091223;
RA Li W., Wang Y., Okamoto M., Crawford N.M., Siddiqi M.Y., Glass A.D.;
RT "Dissection of the AtNRT2.1:AtNRT2.2 inducible high-affinity nitrate
RT transporter gene cluster.";
RL Plant Physiol. 143:425-433(2007).
RN [22]
RP SUBCELLULAR LOCATION.
RX PubMed=17583518; DOI=10.1016/j.plaphy.2007.04.007;
RA Chopin F., Wirth J., Dorbe M.F., Lejay L., Krapp A., Gojon A.,
RA Daniel-Vedele F.;
RT "The Arabidopsis nitrate transporter AtNRT2.1 is targeted to the root
RT plasma membrane.";
RL Plant Physiol. Biochem. 45:630-635(2007).
RN [23]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=19704673; DOI=10.4161/psb.2.4.3870;
RA Orsel M., Chopin F., Leleu O., Smith S.J., Krapp A., Daniel-Vedele F.,
RA Miller A.J.;
RT "Nitrate signaling and the two component high affinity uptake system in
RT Arabidopsis.";
RL Plant Signal. Behav. 2:260-262(2007).
RN [24]
RP SUBCELLULAR LOCATION, SUBUNIT, AND INTERACTION WITH NRT3.1.
RX PubMed=20561257; DOI=10.1111/j.1365-313x.2010.04278.x;
RA Yong Z., Kotur Z., Glass A.D.;
RT "Characterization of an intact two-component high-affinity nitrate
RT transporter from Arabidopsis roots.";
RL Plant J. 63:739-748(2010).
CC -!- FUNCTION: Involved in nitrate transport, but does not seem to be able
CC to mediate transport by its own. Acts as a dual component transporter
CC with NTR3.1. Acts as a repressor of lateral root initiation under high
CC sucrose/low nitrate conditions. {ECO:0000269|PubMed:11553754,
CC ECO:0000269|PubMed:15107992, ECO:0000269|PubMed:16157886,
CC ECO:0000269|PubMed:16415211, ECO:0000269|PubMed:17085507,
CC ECO:0000269|PubMed:19704673}.
CC -!- SUBUNIT: Monomer. Heterotetramer composed of two NRT2.1 and two NRT3.1.
CC Interacts with NRT3.1. {ECO:0000269|PubMed:17012411,
CC ECO:0000269|PubMed:20561257}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:17573350,
CC ECO:0000269|PubMed:17583518, ECO:0000269|PubMed:19704673,
CC ECO:0000269|PubMed:20561257}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:17573350, ECO:0000269|PubMed:17583518,
CC ECO:0000269|PubMed:19704673, ECO:0000269|PubMed:20561257}.
CC -!- TISSUE SPECIFICITY: Root cortical and epidermal cells. Not expressed in
CC new lateral root Primordia. Detected in shoots.
CC {ECO:0000269|PubMed:10205909, ECO:0000269|PubMed:16415211,
CC ECO:0000269|PubMed:17540716, ECO:0000269|PubMed:17573350}.
CC -!- INDUCTION: Up-regulated at transcript level by light and sucrose, and
CC transiently by nitrate, but no changes at protein level. Down-regulated
CC by ammonium, amino acids and N-metabolites resulting from nitrate
CC reduction. Induced by sudden N starvation and by growth on low nitrate
CC concentration. Circadian-regulation. Expression increases during the
CC light phase and decreases during the dark phase.
CC {ECO:0000269|PubMed:10205909, ECO:0000269|PubMed:10417701,
CC ECO:0000269|PubMed:12668777, ECO:0000269|PubMed:15107992,
CC ECO:0000269|PubMed:16415212, ECO:0000269|PubMed:17012411,
CC ECO:0000269|PubMed:17573350, ECO:0000269|PubMed:17897408}.
CC -!- PTM: Might be subject to partial proteolysis at the C-terminus.
CC -!- DISRUPTION PHENOTYPE: Impaired in the high-affinity nitrate transport.
CC Reduced growth on low-nitrate media. Reduced nitrate uptake and
CC increased lateral root initiation. {ECO:0000269|PubMed:11165253,
CC ECO:0000269|PubMed:16157886, ECO:0000269|PubMed:17085507}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR EMBL; Z97058; CAB09794.1; -; mRNA.
DR EMBL; AF019748; AAC35883.1; -; mRNA.
DR EMBL; AF093754; AAC64170.1; -; mRNA.
DR EMBL; AC026875; AAF79826.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28241.1; -; Genomic_DNA.
DR PIR; T51836; T51836.
DR RefSeq; NP_172288.1; NM_100684.3.
DR AlphaFoldDB; O82811; -.
DR SMR; O82811; -.
DR BioGRID; 22568; 1.
DR IntAct; O82811; 1.
DR STRING; 3702.AT1G08090.1; -.
DR TCDB; 2.A.1.8.12; the major facilitator superfamily (mfs).
DR iPTMnet; O82811; -.
DR PaxDb; O82811; -.
DR PRIDE; O82811; -.
DR ProteomicsDB; 249052; -.
DR EnsemblPlants; AT1G08090.1; AT1G08090.1; AT1G08090.
DR GeneID; 837327; -.
DR Gramene; AT1G08090.1; AT1G08090.1; AT1G08090.
DR KEGG; ath:AT1G08090; -.
DR Araport; AT1G08090; -.
DR TAIR; locus:2205190; AT1G08090.
DR eggNOG; ENOG502QPIC; Eukaryota.
DR HOGENOM; CLU_024204_0_0_1; -.
DR InParanoid; O82811; -.
DR OMA; IPCFMFA; -.
DR OrthoDB; 542533at2759; -.
DR PhylomeDB; O82811; -.
DR PRO; PR:O82811; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O82811; baseline and differential.
DR Genevisible; O82811; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; ISS:TAIR.
DR GO; GO:0009705; C:plant-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0071249; P:cellular response to nitrate; IBA:GO_Central.
DR GO; GO:0048527; P:lateral root development; IMP:TAIR.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IMP:TAIR.
DR GO; GO:0010167; P:response to nitrate; IEP:TAIR.
DR CDD; cd17341; MFS_NRT2_like; 1.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR PANTHER; PTHR23515; PTHR23515; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Nitrate assimilation; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..530
FT /note="High-affinity nitrate transporter 2.1"
FT /id="PRO_0000400098"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..149
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 271..290
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 310..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 352..372
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 383..403
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 410..430
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 441..461
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 490..530
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 521
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9LXH0"
FT MUTAGEN 119
FT /note="G->R: In lin1; reduced nitrate uptake and increased
FT lateral root initiation."
FT /evidence="ECO:0000269|PubMed:16157886"
SQ SEQUENCE 530 AA; 57710 MW; A40F435039635090 CRC64;
MGDSTGEPGS SMHGVTGREQ SFAFSVQSPI VHTDKTAKFD LPVDTEHKAT VFKLFSFAKP
HMRTFHLSWI SFSTCFVSTF AAAPLVPIIR ENLNLTKQDI GNAGVASVSG SIFSRLVMGA
VCDLLGPRYG CAFLVMLSAP TVFSMSFVSD AAGFITVRFM IGFCLATFVS CQYWMSTMFN
SQIIGLVNGT AAGWGNMGGG ITQLLMPIVY EIIRRCGSTA FTAWRIAFFV PGWLHIIMGI
LVLNLGQDLP DGNRATLEKA GEVAKDKFGK ILWYAVTNYR TWIFVLLYGY SMGVELSTDN
VIAEYFFDRF HLKLHTAGLI AACFGMANFF ARPAGGYASD FAAKYFGMRG RLWTLWIIQT
AGGLFCVWLG RANTLVTAVV AMVLFSMGAQ AACGATFAIV PFVSRRALGI ISGLTGAGGN
FGSGLTQLLF FSTSHFTTEQ GLTWMGVMIV ACTLPVTLVH FPQWGSMFLP PSTDPVKGTE
AHYYGSEWNE QEKQKNMHQG SLRFAENAKS EGGRRVRSAA TPPENTPNNV
