NRT21_CHLRE
ID NRT21_CHLRE Reviewed; 547 AA.
AC Q39608; A8J4P5;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Nitrate transporter 2.1 {ECO:0000303|PubMed:8567664};
DE AltName: Full=Nitrate assimilation related protein 3 {ECO:0000303|PubMed:8180624};
GN Name=NRT2.1 {ECO:0000303|PubMed:8567664};
GN Synonyms=NAR-3 {ECO:0000303|PubMed:8180624},
GN NAR3 {ECO:0000303|PubMed:8567664};
GN ORFNames=CHLREDRAFT_192090 {ECO:0000312|EMBL:EDP00897.1};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055 {ECO:0000312|EMBL:CAA80925.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=21gr / CC-1690;
RX PubMed=8180624; DOI=10.1111/j.1365-313x.1994.00407.x;
RA Quesada A., Galvan A., Fernandez E.;
RT "Identification of nitrate transporter genes in Chlamydomonas
RT reinhardtii.";
RL Plant J. 5:407-419(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
RN [3]
RP INDUCTION BY NITRATE.
RX PubMed=8111016; DOI=10.1007/bf00040584;
RA Quesada A., Fernandez E.;
RT "Expression of nitrate assimilation related genes in Chlamydomonas
RT reinhardtii.";
RL Plant Mol. Biol. 24:185-194(1994).
RN [4]
RP FUNCTION, AND ACTIVITY REGULATION.
RX PubMed=8567664; DOI=10.1074/jbc.271.4.2088;
RA Galvan A., Quesada A., Fernandez E.;
RT "Nitrate and nitrite are transported by different specific transport
RT systems and by a bispecific transporter in Chlamydomonas reinhardtii.";
RL J. Biol. Chem. 271:2088-2092(1996).
RN [5]
RP INDUCTION BY NITRATE AND NITRITE.
RX PubMed=9648741; DOI=10.1007/s004380050743;
RA Quesada A., Hidalgo J., Fernandez E.;
RT "Three Nrt2 genes are differentially regulated in Chlamydomonas
RT reinhardtii.";
RL Mol. Gen. Genet. 258:373-377(1998).
RN [6]
RP FUNCTION.
RX PubMed=10682832; DOI=10.1016/s0014-5793(00)01085-1;
RA Zhou J.J., Fernandez E., Galvan A., Miller A.J.;
RT "A high affinity nitrate transport system from Chlamydomonas requires two
RT gene products.";
RL FEBS Lett. 466:225-227(2000).
CC -!- FUNCTION: Involved in nitrate transport, but does not seem to be able
CC to mediate transport by its own (PubMed:8180624). Acts as a dual
CC component transporter with NAR2 (system 1) (PubMed:8180624,
CC PubMed:10682832). Imports nitrate with high affinity when expressed
CC with NAR2 in a heterologous system (Xenopus oocytes) (PubMed:10682832).
CC Involved in a high affinity and a high capacity transport specific for
CC both nitrate and nitrite (PubMed:8567664).
CC {ECO:0000269|PubMed:10682832, ECO:0000269|PubMed:8180624,
CC ECO:0000269|PubMed:8567664}.
CC -!- ACTIVITY REGULATION: Nitrite transport mediated by system 1 is very
CC sensitive to inhibition by nitrate. {ECO:0000269|PubMed:8567664}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Up-regulated by nitrate (PubMed:8111016, PubMed:9648741).
CC Up-regulated by nitrite (PubMed:9648741). {ECO:0000269|PubMed:8111016,
CC ECO:0000269|PubMed:9648741}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EDP00897.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; Z25438; CAA80925.1; -; mRNA.
DR EMBL; DS496137; EDP00897.1; ALT_SEQ; Genomic_DNA.
DR PIR; S40142; S40142.
DR AlphaFoldDB; Q39608; -.
DR SMR; Q39608; -.
DR IntAct; Q39608; 1.
DR STRING; 3055.EDP00897; -.
DR TCDB; 2.A.1.8.6; the major facilitator superfamily (mfs).
DR EnsemblPlants; PNW79322; PNW79322; CHLRE_09g410850v5.
DR Gramene; PNW79322; PNW79322; CHLRE_09g410850v5.
DR eggNOG; ENOG502QPIC; Eukaryota.
DR OrthoDB; 542533at2759; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:1990351; C:transporter complex; IMP:UniProtKB.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015113; F:nitrite transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IDA:UniProtKB.
DR GO; GO:0015707; P:nitrite transport; IDA:UniProtKB.
DR CDD; cd17341; MFS_NRT2_like; 1.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR InterPro; IPR004737; NO3_transporter_NarK/NarU-like.
DR PANTHER; PTHR23515; PTHR23515; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00886; 2A0108; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Nitrate assimilation; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..547
FT /note="Nitrate transporter 2.1"
FT /id="PRO_0000438980"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 262..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..358
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..386
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 400..420
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 433..453
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 547 AA; 59131 MW; DCDBF86126EE0B2B CRC64;
MAEKPATVNA ELVKEMDAAP KKYPYSLDSE GKANYCPVWR FTQPHMMAFH LSWICFFMSF
VATFAPASLA PIIRDDLFLT KSQLGNAGVA AVCGAIAARI FMGIVVDSIG PRYGAAATML
MTAPAVFCMA LVTDFSTFAC VRFFIGLSLC MFVCCQFWCG TMFNVKIVGT ANAIAAGWGN
MGGGACHFIM PLIYQGIKDG GVPGYQAWRW AFFVPGGIYI LTATLTLLLG IDHPSGKDYR
DLKKEGTLKA KGAMWPVVKC GLGNYRSWIL ALTYGYSFGV ELTVDNVIVE YLFDQFGLNL
AVAGALGAIF GLMNLFTRAT GGMISDLVAK PFGMRGRIWA LWIIQTLGGI FCIVLGKVSN
SLSSTIVIMI VFSIFCQQAC GLHFGITPFV SRRAYGVVSG LVGAGGNTGA AITQAIWFAG
TAPWQLTLSK ADGFVYMGIM TIGLTLPLFF IWFPMWGSML TGPREGAEEE DYYMREWSAE
EVASGLHQGS MRFAMESKSQ RGTRDKRAAG PARVPQQLRL GARCCQARGG LIRCVLAQSL
GATGCSD
