NRT22_ARATH
ID NRT22_ARATH Reviewed; 522 AA.
AC Q9LMZ9; O82412;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=High-affinity nitrate transporter 2.2;
DE Short=AtNRT2:2;
GN Name=NRT2.2; Synonyms=ACH2, NRT2;2AT; OrderedLocusNames=At1g08100;
GN ORFNames=T6D22.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INDUCTION BY NITRATE, AND TISSUE SPECIFICITY.
RX PubMed=10205909; DOI=10.1046/j.1365-313x.1999.00396.x;
RA Zhuo D., Okamoto M., Vidmar J.J., Glass A.D.;
RT "Regulation of a putative high-affinity nitrate transporter (Nrt2;1At) in
RT roots of Arabidopsis thaliana.";
RL Plant J. 17:563-568(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, AND INDUCTION BY NITRATE.
RX PubMed=11553754; DOI=10.1104/pp.127.1.262;
RA Cerezo M., Tillard P., Filleur S., Munos S., Daniel-Vedele F., Gojon A.;
RT "Major alterations of the regulation of root NO(3)(-) uptake are associated
RT with the mutation of Nrt2.1 and Nrt2.2 genes in Arabidopsis.";
RL Plant Physiol. 127:262-271(2001).
RN [5]
RP GENE FAMILY.
RX PubMed=11912225; DOI=10.1093/jexbot/53.370.825;
RA Orsel M., Filleur S., Fraisier V., Daniel-Vedele F.;
RT "Nitrate transport in plants: which gene and which control?";
RL J. Exp. Bot. 53:825-833(2002).
RN [6]
RP INDUCTION BY NITRATE, TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=12668777; DOI=10.1093/pcp/pcg036;
RA Okamoto M., Vidmar J.J., Glass A.D.;
RT "Regulation of NRT1 and NRT2 gene families of Arabidopsis thaliana:
RT responses to nitrate provision.";
RL Plant Cell Physiol. 44:304-317(2003).
RN [7]
RP FUNCTION, AND INDUCTION BY NITRATE.
RX PubMed=15107992; DOI=10.1007/s00425-004-1266-x;
RA Orsel M., Eulenburg K., Krapp A., Daniel-Vedele F.;
RT "Disruption of the nitrate transporter genes AtNRT2.1 and AtNRT2.2
RT restricts growth at low external nitrate concentration.";
RL Planta 219:714-721(2004).
RN [8]
RP GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17085507; DOI=10.1104/pp.106.091223;
RA Li W., Wang Y., Okamoto M., Crawford N.M., Siddiqi M.Y., Glass A.D.;
RT "Dissection of the AtNRT2.1:AtNRT2.2 inducible high-affinity nitrate
RT transporter gene cluster.";
RL Plant Physiol. 143:425-433(2007).
CC -!- FUNCTION: Involved in high-affinity nitrate transport.
CC {ECO:0000269|PubMed:11553754, ECO:0000269|PubMed:15107992,
CC ECO:0000269|PubMed:17085507}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and shoots.
CC {ECO:0000269|PubMed:10205909, ECO:0000269|PubMed:12668777}.
CC -!- INDUCTION: Transient up-regulation at transcript level by nitrate. No
CC induction by growth on low nitrate concentration.
CC {ECO:0000269|PubMed:10205909, ECO:0000269|PubMed:11553754,
CC ECO:0000269|PubMed:12668777, ECO:0000269|PubMed:15107992}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:17085507}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR EMBL; AF019749; AAC35884.1; -; mRNA.
DR EMBL; AC026875; AAF79836.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28242.1; -; Genomic_DNA.
DR PIR; F86215; F86215.
DR RefSeq; NP_172289.1; NM_100685.1.
DR AlphaFoldDB; Q9LMZ9; -.
DR BioGRID; 22569; 1.
DR STRING; 3702.AT1G08100.1; -.
DR PaxDb; Q9LMZ9; -.
DR PRIDE; Q9LMZ9; -.
DR EnsemblPlants; AT1G08100.1; AT1G08100.1; AT1G08100.
DR GeneID; 837328; -.
DR Gramene; AT1G08100.1; AT1G08100.1; AT1G08100.
DR KEGG; ath:AT1G08100; -.
DR Araport; AT1G08100; -.
DR TAIR; locus:2205195; AT1G08100.
DR eggNOG; ENOG502QPIC; Eukaryota.
DR HOGENOM; CLU_024204_0_0_1; -.
DR InParanoid; Q9LMZ9; -.
DR OMA; DATEHYY; -.
DR OrthoDB; 542533at2759; -.
DR PhylomeDB; Q9LMZ9; -.
DR PRO; PR:Q9LMZ9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LMZ9; differential.
DR Genevisible; Q9LMZ9; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0071249; P:cellular response to nitrate; IBA:GO_Central.
DR GO; GO:0048527; P:lateral root development; IMP:TAIR.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IMP:TAIR.
DR CDD; cd17341; MFS_NRT2_like; 1.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR PANTHER; PTHR23515; PTHR23515; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 2: Evidence at transcript level;
KW Membrane; Nitrate assimilation; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..522
FT /note="High-affinity nitrate transporter 2.2"
FT /id="PRO_0000400099"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 381..401
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 408..428
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 439..459
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 8
FT /note="G -> R (in Ref. 1; AAC35884)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 522 AA; 56616 MW; 51100CF555C15068 CRC64;
MGSTDEPGSS MHGVTGREQS YAFSVDGSEP TNTKKKYNLP VDAEDKATVF KLFSFAKPHM
RTFHLSWISF STCFVSTFAA APLIPIIREN LNLTKHDIGN AGVASVSGSI FSRLVMGAVC
DLLGPRYGCA FLVMLSAPTV FSMSFVSDAA GFITVRFMIG FCLATFVSCQ YWMSTMFNSQ
IIGLVNGTAA GWGNMGGGIT QLLMPIVYEI IRRCGSTAFT AWRIAFFVPG WLHIIMGILV
LTLGQDLPGG NRAAMEKAGE VAKDKFGKIL WYAVTNYRTW IFVLLYGYSM GVELSTDNVI
AEYFFDRFHL KLHTAGIIAA CFGMANFFAR PAGGWASDIA AKRFGMRGRL WTLWIIQTSG
GLFCVWLGRA NTLVTAVVSM VLFSLGAQAA CGATFAIVPF VSRRALGIIS GLTGAGGNFG
SGLTQLVFFS TSRFTTEEGL TWMGVMIVAC TLPVTLIHFP QWGSMFFPPS NDSVDATEHY
YVGEYSKEEQ QIGMHLKSKL FADGAKTEGG SSVHKGNATN NA
