NRT22_CHLRE
ID NRT22_CHLRE Reviewed; 513 AA.
AC Q39609; A8J4P3;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2017, sequence version 2.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=Nitrate transporter 2.2 {ECO:0000303|PubMed:8567664};
DE AltName: Full=Nitrate assimilation related protein 4 {ECO:0000303|PubMed:8180624};
GN Name=NRT2.2 {ECO:0000303|PubMed:8567664};
GN Synonyms=NAR-4 {ECO:0000303|PubMed:8180624},
GN NAR4 {ECO:0000303|PubMed:8567664};
GN ORFNames=CHLREDRAFT_192088 {ECO:0000312|EMBL:EDP00896.1};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055 {ECO:0000312|EMBL:CAA80926.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 276-513, AND FUNCTION.
RC STRAIN=21gr / CC-1690;
RX PubMed=8180624; DOI=10.1111/j.1365-313x.1994.00407.x;
RA Quesada A., Galvan A., Fernandez E.;
RT "Identification of nitrate transporter genes in Chlamydomonas
RT reinhardtii.";
RL Plant J. 5:407-419(1994).
RN [3]
RP INDUCTION BY NITRATE.
RX PubMed=8111016; DOI=10.1007/bf00040584;
RA Quesada A., Fernandez E.;
RT "Expression of nitrate assimilation related genes in Chlamydomonas
RT reinhardtii.";
RL Plant Mol. Biol. 24:185-194(1994).
RN [4]
RP FUNCTION.
RX PubMed=8567664; DOI=10.1074/jbc.271.4.2088;
RA Galvan A., Quesada A., Fernandez E.;
RT "Nitrate and nitrite are transported by different specific transport
RT systems and by a bispecific transporter in Chlamydomonas reinhardtii.";
RL J. Biol. Chem. 271:2088-2092(1996).
RN [5]
RP INDUCTION BY NITRATE.
RX PubMed=9648741; DOI=10.1007/s004380050743;
RA Quesada A., Hidalgo J., Fernandez E.;
RT "Three Nrt2 genes are differentially regulated in Chlamydomonas
RT reinhardtii.";
RL Mol. Gen. Genet. 258:373-377(1998).
CC -!- FUNCTION: Involved in nitrate transport, but does not seem to be able
CC to mediate transport by its own (PubMed:8180624). Acts as a dual
CC component transporter with NAR2 (system 2) (PubMed:8180624). Involved
CC in a high affinity transport specific for nitrate (PubMed:8567664).
CC {ECO:0000269|PubMed:8180624, ECO:0000269|PubMed:8567664}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Up-regulated after a long nitrate induction period.
CC {ECO:0000269|PubMed:8111016, ECO:0000269|PubMed:9648741}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EDP00896.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DS496137; EDP00896.1; ALT_SEQ; Genomic_DNA.
DR EMBL; Z25439; CAA80926.1; -; mRNA.
DR PIR; S40143; S40143.
DR AlphaFoldDB; Q39609; -.
DR SMR; Q39609; -.
DR STRING; 3055.EDP00896; -.
DR TCDB; 2.A.1.8.7; the major facilitator superfamily (mfs).
DR EnsemblPlants; PNW79321; PNW79321; CHLRE_09g410800v5.
DR Gramene; PNW79321; PNW79321; CHLRE_09g410800v5.
DR eggNOG; ENOG502QPIC; Eukaryota.
DR OMA; WLHIIVG; -.
DR OrthoDB; 542533at2759; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:1990351; C:transporter complex; IMP:UniProtKB.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015113; F:nitrite transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IDA:UniProtKB.
DR CDD; cd17341; MFS_NRT2_like; 1.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR InterPro; IPR004737; NO3_transporter_NarK/NarU-like.
DR PANTHER; PTHR23515; PTHR23515; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00886; 2A0108; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Nitrate assimilation; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..513
FT /note="Nitrate transporter 2.2"
FT /id="PRO_0000438981"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 247..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 281..301
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 323..343
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 383..403
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 419..439
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 513 AA; 55790 MW; E45A15D134C45168 CRC64;
MATVEKKYPY ALDSEGKAKY VPVWRFTQPH MMAFHLSWIC FFMSFVATFA PASLAPVIRD
DLFLTKSEVG NAGVSAVCGA IAARLFMGIF VDVVGPRYGA AAAMLMTAPA VFCMALVTDF
STFACVRFFI GLSLCMFVCC QFWCGTMFNV QIVGTANAIA AGWGNMGGGA CHFIMPLIYQ
GIKDGGVPGY QAWRWAFFVP GGIYIVTATL TLLLGIDHPS GKDYRDLKKE GTLKAKGNMW
PVIKCGLGNY RSWILALTYG YSFGVELTVD NVIVEYLFDQ FGLNLAVAGA LGAIFGLMNI
FSRATGGMIS DLIAKPFGMR GRLWVLWITQ TLGGIFCIIM GKVSNSLTAT IVIMIIFSIF
CQQACGMHFG ITPFVSRRAY GVVSGLVGAG GNVGAAITQA IWFSGTATWQ INLSRPDSFV
WMGVQAVALT VAVMFIWFPM WGSMFTGPRE GVEEEDYYLR EWSAEEVAQG LHQGSMRFAM
ESKSQRGYKD KRMLDAVGET ASADVAAVKA TVA
