NRT22_ORYSJ
ID NRT22_ORYSJ Reviewed; 533 AA.
AC P0DKH0; A0A0N7KEJ9; Q6ZH34;
DT 03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT 03-SEP-2014, sequence version 1.
DT 03-AUG-2022, entry version 38.
DE RecName: Full=High-affinity nitrate transporter 2.2;
DE Short=OsNRT2.2;
GN Name=NRT2.2 {ECO:0000305};
GN OrderedLocusNames=Os02g0112600 {ECO:0000312|EMBL:BAS76626.1},
GN LOC_Os02g02190 {ECO:0000305};
GN ORFNames=OJ1399_H05.7 {ECO:0000312|EMBL:BAD07629.1},
GN OsJ_05080 {ECO:0000312|EMBL:EAZ21473.1},
GN P0501G04.36 {ECO:0000312|EMBL:BAD07853.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP FUNCTION.
RA Katayama H., Mori M., Kawamura Y., Tanaka T., Mori M., Hasegawa H.;
RT "Production and characterization of transgenic rice plants carrying a high-
RT affinity nitrate transporter gene (OsNRT2.1).";
RL Breed. Sci. 59:237-243(2009).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=21220781; DOI=10.1093/jxb/erq403;
RA Feng H., Yan M., Fan X., Li B., Shen Q., Miller A.J., Xu G.;
RT "Spatial expression and regulation of rice high-affinity nitrate
RT transporters by nitrogen and carbon status.";
RL J. Exp. Bot. 62:2319-2332(2011).
RN [8]
RP INDUCTION BY NITRATE.
RX PubMed=20862512; DOI=10.1007/s10265-010-0375-9;
RA Takayanagi S., Takagi Y., Araki R., Hasegawa H.;
RT "High-affinity nitrate uptake by rice (Oryza sativa) coleoptiles.";
RL J. Plant Res. 124:305-309(2011).
RN [9]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH NAR2.1, AND
RP INDUCTION.
RX PubMed=21486304; DOI=10.1111/j.1365-3040.2011.02335.x;
RA Yan M., Fan X., Feng H., Miller A.J., Shen Q., Xu G.;
RT "Rice OsNAR2.1 interacts with OsNRT2.1, OsNRT2.2 and OsNRT2.3a nitrate
RT transporters to provide uptake over high and low concentration ranges.";
RL Plant Cell Environ. 34:1360-1372(2011).
CC -!- FUNCTION: Involved in nitrate transport, but does not seem to be able
CC to mediate transport by its own. Acts as a dual component transporter
CC with NAR2.1. Imports nitrate with high affinity when expressed with
CC NAR2.1 in a heterologous system (Xenopus oocytes).
CC {ECO:0000269|PubMed:21220781, ECO:0000269|PubMed:21486304,
CC ECO:0000269|Ref.6}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=34 uM for nitrate {ECO:0000269|PubMed:21486304};
CC -!- SUBUNIT: Heterotetramer composed of two NRT2.2 and two NAR2.1 (By
CC similarity). Interacts with NAR2.1. {ECO:0000250,
CC ECO:0000269|PubMed:21486304}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in primary and lateral roots, root-shoot
CC junction zone, vascular tissues of adventitious root primordia, leaves,
CC embryo and coleoptiles of germinating seeds, husks and anthers.
CC {ECO:0000269|PubMed:21220781}.
CC -!- INDUCTION: Circadian-regulation with a peak in the middle of the
CC morning and at the end of the light period. Induced by nitrate and
CC sucrose in roots. Down-regulated by ammonium, glutamine, asparagine and
CC aspartate in roots. {ECO:0000269|PubMed:20862512,
CC ECO:0000269|PubMed:21220781, ECO:0000269|PubMed:21486304}.
CC -!- MISCELLANEOUS: Plants over-expressing NRT2.2 have accelerated growth
CC under low nitrate conditions (Ref.6).
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR EMBL; AP004090; BAD07629.1; -; Genomic_DNA.
DR EMBL; AP004752; BAD07853.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF07573.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS76626.1; -; Genomic_DNA.
DR EMBL; CM000139; EAZ21473.1; -; Genomic_DNA.
DR EMBL; AK109733; BAG98877.1; -; mRNA.
DR RefSeq; XP_015623596.1; XM_015768110.1.
DR RefSeq; XP_015623638.1; XM_015768152.1.
DR AlphaFoldDB; P0DKH0; -.
DR SMR; P0DKH0; -.
DR STRING; 39947.P0DKH0; -.
DR EnsemblPlants; Os02t0112100-01; Os02t0112100-01; Os02g0112100.
DR EnsemblPlants; Os02t0112600-01; Os02t0112600-01; Os02g0112600.
DR GeneID; 4328051; -.
DR GeneID; 4328052; -.
DR Gramene; Os02t0112100-01; Os02t0112100-01; Os02g0112100.
DR Gramene; Os02t0112600-01; Os02t0112600-01; Os02g0112600.
DR KEGG; osa:4328051; -.
DR KEGG; osa:4328052; -.
DR HOGENOM; CLU_024204_0_0_1; -.
DR OrthoDB; 542533at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR ExpressionAtlas; P0DKH0; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0071249; P:cellular response to nitrate; IBA:GO_Central.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IDA:UniProtKB.
DR CDD; cd17341; MFS_NRT2_like; 1.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR PANTHER; PTHR23515; PTHR23515; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Nitrate assimilation; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..533
FT /note="High-affinity nitrate transporter 2.2"
FT /id="PRO_0000430005"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 319..339
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 384..404
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 533 AA; 57230 MW; 29665CAC0E24750E CRC64;
MDSSTVGAPG SSLHGVTGRE PAFAFSTEVG GEDAAAASKF DLPVDSEHKA KTIRLLSFAN
PHMRTFHLSW ISFFSCFVST FAAAPLVPII RDNLNLTKAD IGNAGVASVS GSIFSRLAMG
AICDMLGPRY GCAFLIMLAA PTVFCMSLID SAAGYIAVRF LIGFSLATFV SCQYWMSTMF
NSKIIGLVNG LAAGWGNMGG GATQLIMPLV YDVIRKCGAT PFTAWRLAYF VPGTLHVVMG
VLVLTLGQDL PDGNLRSLQK KGDVNRDSFS RVLWYAVTNY RTWIFVLLYG YSMGVELTTD
NVIAEYFYDR FDLDLRVAGI IAASFGMANI VARPTGGLLS DLGARYFGMR ARLWNIWILQ
TAGGAFCLLL GRASTLPTSV VCMVLFSFCA QAACGAIFGV IPFVSRRSLG IISGMTGAGG
NFGAGLTQLL FFTSSRYSTG TGLEYMGIMI MACTLPVVLV HFPQWGSMFL PPNAGAEEEH
YYGSEWSEQE KSKGLHGASL KFAENSRSER GRRNVINAAA AAATPPNNSP EHA
