NRT23_ARATH
ID NRT23_ARATH Reviewed; 539 AA.
AC Q9FJH7;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=High affinity nitrate transporter 2.3;
DE Short=AtNRT2:3;
GN Name=NRT2.3; OrderedLocusNames=At5g60780; ORFNames=MAE1.3, MAE1.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP GENE FAMILY.
RX PubMed=11912225; DOI=10.1093/jexbot/53.370.825;
RA Orsel M., Filleur S., Fraisier V., Daniel-Vedele F.;
RT "Nitrate transport in plants: which gene and which control?";
RL J. Exp. Bot. 53:825-833(2002).
RN [5]
RP TISSUE SPECIFICITY, INDUCTION BY NITRATE, AND GENE FAMILY.
RX PubMed=12668777; DOI=10.1093/pcp/pcg036;
RA Okamoto M., Vidmar J.J., Glass A.D.;
RT "Regulation of NRT1 and NRT2 gene families of Arabidopsis thaliana:
RT responses to nitrate provision.";
RL Plant Cell Physiol. 44:304-317(2003).
RN [6]
RP INTERACTION WITH NRT3.1.
RX PubMed=17012411; DOI=10.1104/pp.106.085209;
RA Orsel M., Chopin F., Leleu O., Smith S.J., Krapp A., Daniel-Vedele F.,
RA Miller A.J.;
RT "Characterization of a two-component high-affinity nitrate uptake system in
RT Arabidopsis. Physiology and protein-protein interaction.";
RL Plant Physiol. 142:1304-1317(2006).
RN [7]
RP GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
CC -!- FUNCTION: Involved in high-affinity nitrate transport. Acts as a dual
CC component transporter with NTR3.1 (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with NRT3.1. {ECO:0000269|PubMed:17012411}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and shoots.
CC {ECO:0000269|PubMed:12668777}.
CC -!- INDUCTION: Not induced by nitrate. {ECO:0000269|PubMed:12668777}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR EMBL; DQ447097; ABE66265.1; -; mRNA.
DR EMBL; AB015472; BAB10099.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97376.1; -; Genomic_DNA.
DR RefSeq; NP_200886.1; NM_125471.2.
DR AlphaFoldDB; Q9FJH7; -.
DR SMR; Q9FJH7; -.
DR BioGRID; 21443; 4.
DR IntAct; Q9FJH7; 4.
DR STRING; 3702.AT5G60780.1; -.
DR PaxDb; Q9FJH7; -.
DR PRIDE; Q9FJH7; -.
DR EnsemblPlants; AT5G60780.1; AT5G60780.1; AT5G60780.
DR GeneID; 836199; -.
DR Gramene; AT5G60780.1; AT5G60780.1; AT5G60780.
DR KEGG; ath:AT5G60780; -.
DR Araport; AT5G60780; -.
DR TAIR; locus:2159290; AT5G60780.
DR eggNOG; ENOG502QPIC; Eukaryota.
DR HOGENOM; CLU_024204_0_0_1; -.
DR InParanoid; Q9FJH7; -.
DR OMA; WLHIIVG; -.
DR OrthoDB; 542533at2759; -.
DR PhylomeDB; Q9FJH7; -.
DR PRO; PR:Q9FJH7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FJH7; baseline and differential.
DR Genevisible; Q9FJH7; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0071249; P:cellular response to nitrate; IBA:GO_Central.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IBA:GO_Central.
DR CDD; cd17341; MFS_NRT2_like; 1.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR PANTHER; PTHR23515; PTHR23515; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Membrane; Nitrate assimilation; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..539
FT /note="High affinity nitrate transporter 2.3"
FT /id="PRO_0000400100"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..309
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 324..344
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 359..379
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 384..404
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 448..468
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 501..539
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 501..520
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 539 AA; 58230 MW; 83008E06DD595051 CRC64;
MTHNHSNEEG SIGTSLHGVT AREQVFSFSV DASSQTVQSD DPTAKFALPV DSEHRAKVFN
PLSFAKPHMR AFHLGWLSFF TCFISTFAAA PLVPIIRDNL DLTKTDIGNA GVASVSGAIF
SRLAMGAVCD LLGARYGTAF SLMLTAPTVF SMSFVGGPSG YLGVRFMIGF CLATFVSCQY
WTSVMFNGKI IGLVNGCAGG WGDMGGGVTQ LLMPMVFHVI KLAGATPFMA WRIAFFVPGF
LQVVMGILVL SLGQDLPDGN LSTLQKSGQV SKDKFSKVFW FAVKNYRTWI LFVLYGSSMG
IELTINNVIS GYFYDRFNLK LQTAGIVAAS FGMANFIARP FGGYASDVAA RVFGMRGRLW
TLWIFQTVGA LFCIWLGRAS SLPIAILAMM LFSIGTQAAC GALFGVAPFV SRRSLGLISG
LTGAGGNFGS GLTQLLFFSS ARFSTAEGLS LMGVMAVLCT LPVAFIHFPQ WGSMFLRPST
DGERSQEEYY YGSEWTENEK QQGLHEGSIK FAENSRSERG RKVALANIPT PENGTPSHV
