NRT23_ORYSJ
ID NRT23_ORYSJ Reviewed; 516 AA.
AC Q94JG1; A0A0P0V747; Q5N9S1;
DT 03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=High-affinity nitrate transporter 2.3;
DE Short=OsNRT2.3;
GN Name=NRT2.3; OrderedLocusNames=Os01g0704100, LOC_Os01g50820;
GN ORFNames=OsJ_03170, P0421H07.34-1, P0421H07.34-2, P0684B02.9-1,
GN P0684B02.9-2;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=21220781; DOI=10.1093/jxb/erq403;
RA Feng H., Yan M., Fan X., Li B., Shen Q., Miller A.J., Xu G.;
RT "Spatial expression and regulation of rice high-affinity nitrate
RT transporters by nitrogen and carbon status.";
RL J. Exp. Bot. 62:2319-2332(2011).
RN [8]
RP INDUCTION BY NITRATE.
RX PubMed=20862512; DOI=10.1007/s10265-010-0375-9;
RA Takayanagi S., Takagi Y., Araki R., Hasegawa H.;
RT "High-affinity nitrate uptake by rice (Oryza sativa) coleoptiles.";
RL J. Plant Res. 124:305-309(2011).
RN [9]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH NAR2.1, AND
RP INDUCTION.
RX PubMed=21486304; DOI=10.1111/j.1365-3040.2011.02335.x;
RA Yan M., Fan X., Feng H., Miller A.J., Shen Q., Xu G.;
RT "Rice OsNAR2.1 interacts with OsNRT2.1, OsNRT2.2 and OsNRT2.3a nitrate
RT transporters to provide uptake over high and low concentration ranges.";
RL Plant Cell Environ. 34:1360-1372(2011).
RN [10]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=23093362; DOI=10.1104/pp.112.204461;
RA Tang Z., Fan X., Li Q., Feng H., Miller A.J., Shen Q., Xu G.;
RT "Knockdown of a rice stelar nitrate transporter alters long-distance
RT translocation but not root influx.";
RL Plant Physiol. 160:2052-2063(2012).
CC -!- FUNCTION: Involved in nitrate transport, but does not seem to be able
CC to mediate transport by its own. Acts as a dual component transporter
CC with NAR2.1. Imports nitrate with high affinity when expressed with
CC NAR2.1 in a heterologous system (Xenopus oocytes). Plays a key role in
CC long-distance nitrate transport from root to shoot particularly at low
CC external nitrate supply. {ECO:0000269|PubMed:21220781,
CC ECO:0000269|PubMed:21486304, ECO:0000269|PubMed:23093362}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=310 uM for nitrate {ECO:0000269|PubMed:21486304};
CC -!- SUBUNIT: Heterotetramer composed of two NRT2.3 and two NAR2.1 (By
CC similarity). Isoform 1 interacts with NAR2.1, but not isoform 2.
CC {ECO:0000250, ECO:0000269|PubMed:21486304}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23093362};
CC Multi-pass membrane protein {ECO:0000269|PubMed:23093362}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=OsNRT2.3a;
CC IsoId=Q94JG1-1; Sequence=Displayed;
CC Name=2; Synonyms=OsNRT2.3b;
CC IsoId=Q94JG1-2; Sequence=VSP_055400;
CC -!- TISSUE SPECIFICITY: Expressed in the stelar cells of both primary and
CC lateral roots, particularly at the site of lateral root emergence,
CC root-shoot junction zone, vascular tissues of adventitious root
CC primordia, leaves, germ tips and seed scutellum.
CC {ECO:0000269|PubMed:21220781, ECO:0000269|PubMed:23093362}.
CC -!- INDUCTION: Circadian-regulation with a peak in the middle of the
CC morning and at the end of the light period. Isoforms 1 and 2 are
CC induced by sucrose in roots. Isoform 1 is induced by nitrate in roots,
CC but not isoform 2. Isoform 1 is down-regulated by ammonium, glutatmate
CC and aspartate in roots, but not isoform 2.
CC {ECO:0000269|PubMed:20862512, ECO:0000269|PubMed:21220781,
CC ECO:0000269|PubMed:21486304}.
CC -!- MISCELLANEOUS: Plants silencing isoform 1 of NTR2.3 have impaired xylem
CC loading of nitrate and decreased plant growth at low nitrate supply
CC (0.5 mM). {ECO:0000305|PubMed:23093362}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR EMBL; AP003023; BAB44020.1; -; Genomic_DNA.
DR EMBL; AP003023; BAD81572.1; -; Genomic_DNA.
DR EMBL; AP003245; BAB92299.1; -; Genomic_DNA.
DR EMBL; AP003245; BAD81794.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF05917.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS73916.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS73917.1; -; Genomic_DNA.
DR EMBL; CM000138; EEE55262.1; -; Genomic_DNA.
DR EMBL; AK109776; BAG98894.1; -; mRNA.
DR RefSeq; XP_015628524.1; XM_015773038.1. [Q94JG1-1]
DR AlphaFoldDB; Q94JG1; -.
DR SMR; Q94JG1; -.
DR STRING; 4530.OS01T0704100-02; -.
DR PaxDb; Q94JG1; -.
DR PRIDE; Q94JG1; -.
DR EnsemblPlants; Os01t0704100-01; Os01t0704100-01; Os01g0704100. [Q94JG1-2]
DR EnsemblPlants; Os01t0704100-02; Os01t0704100-02; Os01g0704100. [Q94JG1-1]
DR GeneID; 4324249; -.
DR Gramene; Os01t0704100-01; Os01t0704100-01; Os01g0704100. [Q94JG1-2]
DR Gramene; Os01t0704100-02; Os01t0704100-02; Os01g0704100. [Q94JG1-1]
DR KEGG; osa:4324249; -.
DR eggNOG; ENOG502QPIC; Eukaryota.
DR HOGENOM; CLU_024204_0_0_1; -.
DR InParanoid; Q94JG1; -.
DR OrthoDB; 542533at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000007752; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q94JG1; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0071249; P:cellular response to nitrate; IBA:GO_Central.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IDA:UniProtKB.
DR CDD; cd17341; MFS_NRT2_like; 1.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR PANTHER; PTHR23515; PTHR23515; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Membrane; Nitrate assimilation;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..516
FT /note="High-affinity nitrate transporter 2.3"
FT /id="PRO_0000430006"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..319
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 335..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 367..387
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 489..516
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 64..93
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_055400"
SQ SEQUENCE 516 AA; 55407 MW; 00E912C960AF558E CRC64;
MEAKPVAMEV EGVEAAGGKP RFRMPVDSDL KATEFWLFSF ARPHMASFHM AWFSFFCCFV
STFAAPPLLP LIRDTLGLTA TDIGNAGIAS VSGAVFARLA MGTACDLVGP RLASASLILL
TTPAVYCSSI IQSPSGYLLV RFFTGISLAS FVSAQFWMSS MFSAPKVGLA NGVAGGWGNL
GGGAVQLLMP LVYEAIHKIG STPFTAWRIA FFIPGLMQTF SAIAVLAFGQ DMPGGNYGKL
HKTGDMHKDS FGNVLRHALT NYRGWILALT YGYSFGVELT IDNVVHQYFY DRFDVNLQTA
GLIAASFGMA NIISRPGGGL LSDWLSSRYG MRGRLWGLWT VQTIGGVLCV VLGIVDFSFA
ASVAVMVLFS FFVQAACGLT FGIVPFVSRR SLGLISGMTG GGGNVGAVLT QYIFFHGTKY
KTETGIKYMG LMIIACTLPV MLIYFPQWGG MLVGPRKGAT AEEYYSREWS DHEREKGFNA
ASVRFAENSV REGGRSSANG GQPRHTVPVD ASPAGV
