NRT24_ORYSJ
ID NRT24_ORYSJ Reviewed; 485 AA.
AC A2ZU80;
DT 03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT 03-SEP-2014, sequence version 2.
DT 03-AUG-2022, entry version 53.
DE RecName: Full=Probable high-affinity nitrate transporter 2.4;
DE Short=OsNRT2.4;
GN Name=NRT2.4; OrderedLocusNames=Os01g0547600, LOC_Os01g36720;
GN ORFNames=OsJ_02167, OSJNBa0026J14;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=21220781; DOI=10.1093/jxb/erq403;
RA Feng H., Yan M., Fan X., Li B., Shen Q., Miller A.J., Xu G.;
RT "Spatial expression and regulation of rice high-affinity nitrate
RT transporters by nitrogen and carbon status.";
RL J. Exp. Bot. 62:2319-2332(2011).
RN [7]
RP INDUCTION.
RX PubMed=21486304; DOI=10.1111/j.1365-3040.2011.02335.x;
RA Yan M., Fan X., Feng H., Miller A.J., Shen Q., Xu G.;
RT "Rice OsNAR2.1 interacts with OsNRT2.1, OsNRT2.2 and OsNRT2.3a nitrate
RT transporters to provide uptake over high and low concentration ranges.";
RL Plant Cell Environ. 34:1360-1372(2011).
CC -!- FUNCTION: Involved in nitrate transport. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the base of the lateral root
CC primordia, root-shoot junction zone, leaves, ends of the husk and
CC vascular tissue of the anthers. {ECO:0000269|PubMed:21220781}.
CC -!- INDUCTION: Circadian-regulation with a peak in the middle of the
CC morning. Induced by nitrate and sucrose in roots. Down-regulated by
CC glutamine, gluatamate, asparagine and aspartate in roots.
CC {ECO:0000269|PubMed:21220781, ECO:0000269|PubMed:21486304}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAZ12277.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP004231; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP008207; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP014957; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CM000138; EAZ12277.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_015636108.1; XM_015780622.1.
DR AlphaFoldDB; A2ZU80; -.
DR SMR; A2ZU80; -.
DR STRING; 39947.A2ZU80; -.
DR PaxDb; A2ZU80; -.
DR PRIDE; A2ZU80; -.
DR EnsemblPlants; Os01t0547600-01; Os01t0547600-01; Os01g0547600.
DR GeneID; 107277478; -.
DR Gramene; Os01t0547600-01; Os01t0547600-01; Os01g0547600.
DR KEGG; osa:107277478; -.
DR eggNOG; ENOG502R19F; Eukaryota.
DR OrthoDB; 542533at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000007752; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0071249; P:cellular response to nitrate; IBA:GO_Central.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IBA:GO_Central.
DR CDD; cd17341; MFS_NRT2_like; 1.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR PANTHER; PTHR23515; PTHR23515; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Nitrate assimilation; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..485
FT /note="Probable high-affinity nitrate transporter 2.4"
FT /id="PRO_0000430007"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 119..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..290
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..327
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 341..361
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 405..425
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 435..455
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 485 AA; 50199 MW; FC7F5156A0A9E4FD CRC64;
MVAMEKKTKL VEEEDGCYYY DYGGYGDGVV DDEGRATELR PMALSRPHTQ AFHLAWMSLF
ACFFAAFAAP PILPAMRPAL VLAPSDASAA AVASLSATLV GRLAMGPACD LLGPRRASGV
ASLVCALALA LAAVFASSPA GFVALRFVAG LSLANFVANQ HWMSRIFAPS AVGLANAVAA
GWANVGSAAA QVVMPVAYDA VVLRLGVPVT VAWRVTYLLP CAMLVTTGLA VLAFPYDLPG
GGGGRCPGGG GGRRRSFWAV VRGGVGDYRA WLLGLTYGHC YGVELIMENV AADFFRRRFR
LPMEAAGAAA ACFGAMNAVA RPAGGVASDE VARRFGMRGR LWALWAVQSA GAALCVLVGR
MGAAEAPSLA ATVAVMVACA AFVQAASGLT FGIVPFVCKR SLGVVSGMTA SGGAVGAIVT
NRLFFSGSRY TVEEAISCTG ITSLLCTLPV ALIHFRRQGG MFCGPSATID GDGDVDDDDD
YMLLK
