NRT25_ARATH
ID NRT25_ARATH Reviewed; 502 AA.
AC Q9LPV5;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=High affinity nitrate transporter 2.5;
DE Short=AtNRT2:5;
GN Name=NRT2.5; OrderedLocusNames=At1g12940; ORFNames=F13K23.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=11912225; DOI=10.1093/jexbot/53.370.825;
RA Orsel M., Filleur S., Fraisier V., Daniel-Vedele F.;
RT "Nitrate transport in plants: which gene and which control?";
RL J. Exp. Bot. 53:825-833(2002).
RN [4]
RP TISSUE SPECIFICITY, INDUCTION BY NITRATE, AND GENE FAMILY.
RX PubMed=12668777; DOI=10.1093/pcp/pcg036;
RA Okamoto M., Vidmar J.J., Glass A.D.;
RT "Regulation of NRT1 and NRT2 gene families of Arabidopsis thaliana:
RT responses to nitrate provision.";
RL Plant Cell Physiol. 44:304-317(2003).
RN [5]
RP FUNCTION, AND INDUCTION BY NITRATE.
RX PubMed=15107992; DOI=10.1007/s00425-004-1266-x;
RA Orsel M., Eulenburg K., Krapp A., Daniel-Vedele F.;
RT "Disruption of the nitrate transporter genes AtNRT2.1 and AtNRT2.2
RT restricts growth at low external nitrate concentration.";
RL Planta 219:714-721(2004).
RN [6]
RP INDUCTION BY PHYLLOBACTERIUM.
RX PubMed=16160849; DOI=10.1007/s00425-005-0106-y;
RA Mantelin S., Desbrosses G., Larcher M., Tranbarger T.J., Cleyet-Marel J.C.,
RA Touraine B.;
RT "Nitrate-dependent control of root architecture and N nutrition are altered
RT by a plant growth-promoting Phyllobacterium sp.";
RL Planta 223:591-603(2006).
RN [7]
RP GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [8]
RP TISSUE SPECIFICITY, AND INDUCTION BY IMBIBITION.
RX PubMed=17540716; DOI=10.1105/tpc.107.050542;
RA Chopin F., Orsel M., Dorbe M.F., Chardon F., Truong H.N., Miller A.J.,
RA Krapp A., Daniel-Vedele F.;
RT "The Arabidopsis ATNRT2.7 nitrate transporter controls nitrate content in
RT seeds.";
RL Plant Cell 19:1590-1602(2007).
RN [9]
RP INTERACTION WITH NRT3.1.
RX PubMed=22432443; DOI=10.1111/j.1469-8137.2012.04094.x;
RA Kotur Z., Mackenzie N., Ramesh S., Tyerman S.D., Kaiser B.N., Glass A.D.;
RT "Nitrate transport capacity of the Arabidopsis thaliana NRT2 family members
RT and their interactions with AtNAR2.1.";
RL New Phytol. 194:724-731(2012).
RN [10]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=23398541; DOI=10.1111/nph.12158;
RA Kechid M., Desbrosses G., Rokhsi W., Varoquaux F., Djekoun A., Touraine B.;
RT "The NRT2.5 and NRT2.6 genes are involved in growth promotion of
RT Arabidopsis by the plant growth-promoting rhizobacterium (PGPR) strain
RT Phyllobacterium brassicacearum STM196.";
RL New Phytol. 198:514-524(2013).
RN [11]
RP FUNCTION, INDUCTION BY NITROGEN, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=25065551; DOI=10.1111/tpj.12626;
RA Lezhneva L., Kiba T., Feria-Bourrellier A.B., Lafouge F., Boutet-Mercey S.,
RA Zoufan P., Sakakibara H., Daniel-Vedele F., Krapp A.;
RT "The Arabidopsis nitrate transporter NRT2.5 plays a role in nitrate
RT acquisition and remobilization in nitrogen-starved plants.";
RL Plant J. 80:230-241(2014).
RN [12]
RP FUNCTION, INDUCTION BY NITRATE, SUBUNIT, INTERACTION WITH NRT3.1,
RP DISRUPTION PHENOTYPE, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=25474587; DOI=10.1111/pce.12496;
RA Kotur Z., Glass A.D.;
RT "A 150 kDa plasma membrane complex of AtNRT2.5 and AtNAR2.1 is the major
RT contributor to constitutive high-affinity nitrate influx in Arabidopsis
RT thaliana.";
RL Plant Cell Environ. 38:1490-1502(2015).
CC -!- FUNCTION: Nitrate transporter involved in the constitutive high-
CC affinity transport system (cHATS) under long-term N starvation
CC conditions (PubMed:15107992, PubMed:25065551). Predominantly expressed
CC in roots of nitrate-deprived plants as a 150 kDa molecular complex with
CC NRT3.1 representing the major contributor to cHATS influx
CC (PubMed:25474587). The principal role of this cHATS is to enable roots
CC previously deprived of nitrate to absorb this ion and initiate
CC induction of nitrate-inducible genes (PubMed:25474587). Not involved in
CC transfer of nitrate from roots to shoots (PubMed:25474587). Contributes
CC to phloem loading of nitrate in shoots during N starvation, but not
CC required for growth and nitrate uptake in young plants
CC (PubMed:25065551). Required for the nitrate uptake-independent plant
CC growth promotion and lateral root response to the rhizospheric
CC Phyllobacterium (PubMed:23398541). Might be involved in the transfer of
CC nitrate from stored pools to cytoplasm (PubMed:15107992).
CC {ECO:0000269|PubMed:15107992, ECO:0000269|PubMed:23398541,
CC ECO:0000269|PubMed:25065551, ECO:0000269|PubMed:25474587}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=10.75 uM for nitrate {ECO:0000269|PubMed:25474587};
CC -!- SUBUNIT: Oligomeric molecular complex with NRT3.1 (PubMed:22432443,
CC PubMed:25474587). {ECO:0000269|PubMed:22432443,
CC ECO:0000269|PubMed:25474587}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:25065551};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, shoots and seeds
CC (PubMed:12668777, PubMed:17540716). Expressed in leaves
CC (PubMed:23398541). Expressed in root hair zone of the primary root and
CC the lateral roots, but not in the lateral root tip or in older parts of
CC the roots (PubMed:25065551). Detected mainly in the epidermis and the
CC cortex (PubMed:25065551). Expressed in shoots only in higher-order
CC veins (PubMed:25065551). {ECO:0000269|PubMed:12668777,
CC ECO:0000269|PubMed:17540716, ECO:0000269|PubMed:23398541,
CC ECO:0000269|PubMed:25065551}.
CC -!- INDUCTION: Down-regulated by nitrate and by imbibition
CC (PubMed:12668777, PubMed:17540716, PubMed:25474587). No induction by
CC growth on low nitrate concentration, but strong induction in mutant
CC with disruption in both NRT2.1 and NRT2.2 (PubMed:15107992). However,
CC this overexpression could not restore the nitrate influx
CC (PubMed:15107992). Induced under long-term nitrogen starvation
CC (PubMed:25065551). Strongly up-regulated upon inoculation with the
CC plant growth-promoting rhizobacteria Phyllobacterium (PubMed:16160849).
CC {ECO:0000269|PubMed:12668777, ECO:0000269|PubMed:15107992,
CC ECO:0000269|PubMed:16160849, ECO:0000269|PubMed:17540716,
CC ECO:0000269|PubMed:25065551, ECO:0000269|PubMed:25474587}.
CC -!- DISRUPTION PHENOTYPE: Loss of plant growth and root system architecture
CC responses to the rhizospheric Phyllobacterium (PubMed:23398541).
CC Decreased nitrate uptake in the high-affinity range (PubMed:25065551,
CC PubMed:25474587). {ECO:0000269|PubMed:23398541,
CC ECO:0000269|PubMed:25065551, ECO:0000269|PubMed:25474587}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR EMBL; AC012187; AAF78499.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28952.1; -; Genomic_DNA.
DR PIR; C86263; C86263.
DR RefSeq; NP_172754.1; NM_101165.3.
DR AlphaFoldDB; Q9LPV5; -.
DR BioGRID; 23092; 1.
DR STRING; 3702.AT1G12940.1; -.
DR PaxDb; Q9LPV5; -.
DR PRIDE; Q9LPV5; -.
DR ProteomicsDB; 250603; -.
DR EnsemblPlants; AT1G12940.1; AT1G12940.1; AT1G12940.
DR GeneID; 837852; -.
DR Gramene; AT1G12940.1; AT1G12940.1; AT1G12940.
DR KEGG; ath:AT1G12940; -.
DR Araport; AT1G12940; -.
DR TAIR; locus:2010391; AT1G12940.
DR eggNOG; ENOG502QPIC; Eukaryota.
DR HOGENOM; CLU_024204_0_0_1; -.
DR InParanoid; Q9LPV5; -.
DR OMA; YCQYYSV; -.
DR OrthoDB; 542533at2759; -.
DR PhylomeDB; Q9LPV5; -.
DR PRO; PR:Q9LPV5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LPV5; baseline and differential.
DR Genevisible; Q9LPV5; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0071249; P:cellular response to nitrate; IBA:GO_Central.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IBA:GO_Central.
DR CDD; cd17341; MFS_NRT2_like; 1.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR PANTHER; PTHR23515; PTHR23515; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Nitrate assimilation; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..502
FT /note="High affinity nitrate transporter 2.5"
FT /id="PRO_0000400102"
FT TRANSMEM 51..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 300..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 334..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 393..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 423..443
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 477..502
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 481..502
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 502 AA; 54262 MW; 624B53EA3A82108C CRC64;
MEVEGKGGEA GTTTTTAPRR FALPVDAENK ATTFRLFSVA KPHMRAFHLS WFQFFCCFVS
TFAAPPLLPV IRENLNLTAT DIGNAGIASV SGAVFARIVM GTACDLFGPR LASAALTLST
APAVYFTAGI KSPIGFIMVR FFAGFSLATF VSTQFWMSSM FSGPVVGSAN GIAAGWGNLG
GGATQLIMPI VFSLIRNMGA TKFTAWRIAF FIPGLFQTLS AFAVLLFGQD LPDGDYWAMH
KSGEREKDDV GKVISNGIKN YRGWITALAY GYCFGVELTI DNIIAEYFFD RFHLKLQTAG
IIAASFGLAN FFARPGGGIF SDFMSRRFGM RGRLWAWWIV QTSGGVLCAC LGQISSLTVS
IIVMLVFSVF VQAACGLTFG VVPFISRRSL GVVSGMTGAG GNVGAVLTQL IFFKGSTYTR
ETGITLMGVM SIACSLPICL IYFPQWGGMF CGPSSKKVTE EDYYLAEWND EEKEKNLHIG
SQKFAETSIS ERGRATTTHP QT
