NRT26_ARATH
ID NRT26_ARATH Reviewed; 542 AA.
AC Q9LXH0;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=High affinity nitrate transporter 2.6;
DE Short=AtNRT2:6;
GN Name=NRT2.6; OrderedLocusNames=At3g45060; ORFNames=F14D17.130;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Cheuk R., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=11912225; DOI=10.1093/jexbot/53.370.825;
RA Orsel M., Filleur S., Fraisier V., Daniel-Vedele F.;
RT "Nitrate transport in plants: which gene and which control?";
RL J. Exp. Bot. 53:825-833(2002).
RN [5]
RP TISSUE SPECIFICITY, INDUCTION BY NITRATE, AND GENE FAMILY.
RX PubMed=12668777; DOI=10.1093/pcp/pcg036;
RA Okamoto M., Vidmar J.J., Glass A.D.;
RT "Regulation of NRT1 and NRT2 gene families of Arabidopsis thaliana:
RT responses to nitrate provision.";
RL Plant Cell Physiol. 44:304-317(2003).
RN [6]
RP FUNCTION, AND INDUCTION BY NITRATE.
RX PubMed=15107992; DOI=10.1007/s00425-004-1266-x;
RA Orsel M., Eulenburg K., Krapp A., Daniel-Vedele F.;
RT "Disruption of the nitrate transporter genes AtNRT2.1 and AtNRT2.2
RT restricts growth at low external nitrate concentration.";
RL Planta 219:714-721(2004).
RN [7]
RP INDUCTION BY PHYLLOBACTERIUM.
RX PubMed=16160849; DOI=10.1007/s00425-005-0106-y;
RA Mantelin S., Desbrosses G., Larcher M., Tranbarger T.J., Cleyet-Marel J.C.,
RA Touraine B.;
RT "Nitrate-dependent control of root architecture and N nutrition are altered
RT by a plant growth-promoting Phyllobacterium sp.";
RL Planta 223:591-603(2006).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-533 AND SER-538, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=17869214; DOI=10.1016/j.bbrc.2007.08.177;
RA Hem S., Rofidal V., Sommerer N., Rossignol M.;
RT "Novel subsets of the Arabidopsis plasmalemma phosphoproteome identify
RT phosphorylation sites in secondary active transporters.";
RL Biochem. Biophys. Res. Commun. 363:375-380(2007).
RN [9]
RP GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [10]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=23398541; DOI=10.1111/nph.12158;
RA Kechid M., Desbrosses G., Rokhsi W., Varoquaux F., Djekoun A., Touraine B.;
RT "The NRT2.5 and NRT2.6 genes are involved in growth promotion of
RT Arabidopsis by the plant growth-promoting rhizobacterium (PGPR) strain
RT Phyllobacterium brassicacearum STM196.";
RL New Phytol. 198:514-524(2013).
CC -!- FUNCTION: Involved in high-affinity nitrate transport
CC (PubMed:15107992). Required for the nitrate uptake-independent plant
CC growth promotion and lateral root response to the rhizospheric
CC Phyllobacterium (PubMed:23398541). {ECO:0000269|PubMed:15107992,
CC ECO:0000269|PubMed:23398541}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and shoots (PubMed:12668777).
CC Expressed in leaves (PubMed:23398541). {ECO:0000269|PubMed:12668777,
CC ECO:0000269|PubMed:23398541}.
CC -!- INDUCTION: Not induced by nitrate or by growth on low nitrate
CC concentration (PubMed:12668777, PubMed:15107992). Strongly up-regulated
CC upon inoculation with the plant growth-promoting rhizobacteria
CC Phyllobacterium (PubMed:16160849). {ECO:0000269|PubMed:12668777,
CC ECO:0000269|PubMed:15107992, ECO:0000269|PubMed:16160849}.
CC -!- DISRUPTION PHENOTYPE: Loss of plant growth and root system architecture
CC responses to the rhizospheric Phyllobacterium.
CC {ECO:0000269|PubMed:23398541}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR EMBL; AL353992; CAB89321.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77987.1; -; Genomic_DNA.
DR EMBL; BT011687; AAS49050.1; -; mRNA.
DR EMBL; BT015382; AAU05505.1; -; mRNA.
DR PIR; T48982; T48982.
DR RefSeq; NP_190092.1; NM_114375.5.
DR AlphaFoldDB; Q9LXH0; -.
DR SMR; Q9LXH0; -.
DR BioGRID; 8961; 3.
DR IntAct; Q9LXH0; 1.
DR STRING; 3702.AT3G45060.1; -.
DR iPTMnet; Q9LXH0; -.
DR PaxDb; Q9LXH0; -.
DR PRIDE; Q9LXH0; -.
DR ProteomicsDB; 250477; -.
DR EnsemblPlants; AT3G45060.1; AT3G45060.1; AT3G45060.
DR GeneID; 823641; -.
DR Gramene; AT3G45060.1; AT3G45060.1; AT3G45060.
DR KEGG; ath:AT3G45060; -.
DR Araport; AT3G45060; -.
DR TAIR; locus:2075909; AT3G45060.
DR eggNOG; ENOG502QPIC; Eukaryota.
DR HOGENOM; CLU_024204_0_0_1; -.
DR InParanoid; Q9LXH0; -.
DR OMA; GICTYLF; -.
DR OrthoDB; 542533at2759; -.
DR PhylomeDB; Q9LXH0; -.
DR PRO; PR:Q9LXH0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LXH0; baseline and differential.
DR Genevisible; Q9LXH0; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0071249; P:cellular response to nitrate; IBA:GO_Central.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IBA:GO_Central.
DR CDD; cd17341; MFS_NRT2_like; 1.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR PANTHER; PTHR23515; PTHR23515; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Membrane; Nitrate assimilation; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..542
FT /note="High affinity nitrate transporter 2.6"
FT /id="PRO_0000400103"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..312
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 362..382
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 421..441
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 451..471
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 516..542
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 533
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17869214"
FT MOD_RES 538
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17869214"
SQ SEQUENCE 542 AA; 58637 MW; C9E53006DC67C03B CRC64;
MAHNHSNEDG SIGTSLHGVT AREQVFSFSV QEDVPSSQAV RTNDPTAKFA LPVDSEHRAK
VFKPLSFAKP HMRAFHLGWI SFFTCFISTF AAAPLVPVIR DNLDLTKTDI GNAGVASVSG
AIFSRLAMGA VCDLLGARYG TAFSLMLTAP AVFSMSFVAD AGSYLAVRFM IGFCLATFVS
CQYWTSVMFT GKIIGLVNGC AGGWGDMGGG VTQLLMPMVF HVIKLTGATP FTAWRFAFFI
PGILQIVMGI LVLTLGQDLP DGNLSTLQKS GQVSKDKFSK VFWFAVKNYR TWILFMLYGF
SMGVELTINN VISGYFYDRF NLTLHTAGII AASFGMANFF ARPFGGYASD VAARLFGMRG
RLWILWILQT VGALFCIWLG RASSLPIAIL AMMLFSMGTQ AACGALFGVA PFVSRRSLGL
ISGLTGAGGN FGSGVTQLLF FSSSRFSTAE GLSLMGVMAV VCSLPVAFIH FPQWGSMFLR
PSQDGEKSKE EHYYGAEWTE EEKSLGLHEG SIKFAENSRS ERGRKAMLAD IPTPETGSPA
HV
