NRT27_ARATH
ID NRT27_ARATH Reviewed; 493 AA.
AC Q9LYK2;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=High affinity nitrate transporter 2.7;
DE Short=AtNRT2:7;
GN Name=NRT2.7; OrderedLocusNames=At5g14570; ORFNames=T15N1.60;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY.
RX PubMed=11912225; DOI=10.1093/jexbot/53.370.825;
RA Orsel M., Filleur S., Fraisier V., Daniel-Vedele F.;
RT "Nitrate transport in plants: which gene and which control?";
RL J. Exp. Bot. 53:825-833(2002).
RN [5]
RP TISSUE SPECIFICITY, INDUCTION BY NITRATE, AND GENE FAMILY.
RX PubMed=12668777; DOI=10.1093/pcp/pcg036;
RA Okamoto M., Vidmar J.J., Glass A.D.;
RT "Regulation of NRT1 and NRT2 gene families of Arabidopsis thaliana:
RT responses to nitrate provision.";
RL Plant Cell Physiol. 44:304-317(2003).
RN [6]
RP FUNCTION, AND INDUCTION BY NITRATE.
RX PubMed=15107992; DOI=10.1007/s00425-004-1266-x;
RA Orsel M., Eulenburg K., Krapp A., Daniel-Vedele F.;
RT "Disruption of the nitrate transporter genes AtNRT2.1 and AtNRT2.2
RT restricts growth at low external nitrate concentration.";
RL Planta 219:714-721(2004).
RN [7]
RP GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [8]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION BY IMBIBITION,
RP SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=17540716; DOI=10.1105/tpc.107.050542;
RA Chopin F., Orsel M., Dorbe M.F., Chardon F., Truong H.N., Miller A.J.,
RA Krapp A., Daniel-Vedele F.;
RT "The Arabidopsis ATNRT2.7 nitrate transporter controls nitrate content in
RT seeds.";
RL Plant Cell 19:1590-1602(2007).
CC -!- FUNCTION: Involved in high-affinity nitrate transport. Controls nitrate
CC content in seeds. {ECO:0000269|PubMed:15107992,
CC ECO:0000269|PubMed:17540716}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:17540716};
CC Multi-pass membrane protein {ECO:0000269|PubMed:17540716}.
CC -!- TISSUE SPECIFICITY: Expressed in seeds, leaves and shoots. Lower
CC expression in roots. {ECO:0000269|PubMed:12668777,
CC ECO:0000269|PubMed:17540716}.
CC -!- DEVELOPMENTAL STAGE: Expressed mainly at the end of seed maturation
CC when the tissue is completely dry. {ECO:0000269|PubMed:17540716}.
CC -!- INDUCTION: Not induced by nitrate or by growth on low nitrate
CC concentration. Down-regulated by imbibition.
CC {ECO:0000269|PubMed:12668777, ECO:0000269|PubMed:15107992,
CC ECO:0000269|PubMed:17540716}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype during vegetative growth. No
CC effect on root nitrate influx. Decreased nitrate content in mature
CC seeds and delayed germination. {ECO:0000269|PubMed:17540716}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR EMBL; AL163792; CAB87624.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92049.1; -; Genomic_DNA.
DR EMBL; AY035065; AAK59570.1; -; mRNA.
DR EMBL; AY056363; AAL07249.1; -; mRNA.
DR PIR; T48630; T48630.
DR RefSeq; NP_196961.1; NM_121461.3.
DR AlphaFoldDB; Q9LYK2; -.
DR BioGRID; 16585; 2.
DR IntAct; Q9LYK2; 3.
DR STRING; 3702.AT5G14570.1; -.
DR PaxDb; Q9LYK2; -.
DR PRIDE; Q9LYK2; -.
DR ProteomicsDB; 250478; -.
DR EnsemblPlants; AT5G14570.1; AT5G14570.1; AT5G14570.
DR GeneID; 831308; -.
DR Gramene; AT5G14570.1; AT5G14570.1; AT5G14570.
DR KEGG; ath:AT5G14570; -.
DR Araport; AT5G14570; -.
DR TAIR; locus:2222652; AT5G14570.
DR eggNOG; ENOG502QPIC; Eukaryota.
DR HOGENOM; CLU_024204_0_0_1; -.
DR InParanoid; Q9LYK2; -.
DR OMA; SFWAVVR; -.
DR OrthoDB; 542533at2759; -.
DR PhylomeDB; Q9LYK2; -.
DR PRO; PR:Q9LYK2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LYK2; baseline and differential.
DR Genevisible; Q9LYK2; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0071249; P:cellular response to nitrate; IBA:GO_Central.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IDA:TAIR.
DR CDD; cd17341; MFS_NRT2_like; 1.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR PANTHER; PTHR23515; PTHR23515; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Membrane; Nitrate assimilation; Reference proteome; Transmembrane;
KW Transmembrane helix; Vacuole.
FT CHAIN 1..493
FT /note="High affinity nitrate transporter 2.7"
FT /id="PRO_0000400104"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..319
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 341..361
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 368..388
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 400..420
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 431..451
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 493 AA; 52678 MW; 1D425DE65D3D55E0 CRC64;
MEPSQRNTKP PSFSDSTIPV DSDGRATVFR PFSLSSPHSR AFHLAWLSLF SCFFSTFSIP
PLVPVISSDL NLSASTVSAA GIASFAGSIF SRLAMGPLCD LIGPRTSSAI LSFLTAPVIL
SASLVSSPTS FILVRFFVGF SLANFVANQY WMSSMFSGNV IGLANGVSAG WANVGAGISQ
LLMPLIYSTI AEFLPRAVAW RVSFVFPAIF QVTTAVLVLL YGQDTPHGNR KNSNQNKLTI
PEEEEVLVVE EDERSSFVEI LIGGLGNYRA WILALLYGYS YGVELTTDNV IAGYFYERFG
VNLEAAGTIA ASFGISNIAS RPAGGMISDA LGKRFGMRGR LWGLWIVQSV AGLLCVLLGR
VNSLWGSILV MWVFSVFVQA ASGLVFGVVP FVSTRSLGVV AGITGSGGTV GAVVTQFLLF
SGDDVRKQRS ISLMGLMTFV FALSVTSIYF PQWGGMCCGP SSSSEEEDIS RGLLVEDEDE
EGKVVSGSLR PVC
