NRT31_ARATH
ID NRT31_ARATH Reviewed; 210 AA.
AC Q9FGS5; Q8LE73; Q9ARM2;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=High-affinity nitrate transporter 3.1;
DE AltName: Full=Protein WOUND-RESPONSIVE 3;
DE Flags: Precursor;
GN Name=NRT3.1; Synonyms=NAR2.1, NAR2.2, WR3; OrderedLocusNames=At5g50200;
GN ORFNames=K6A12.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Zhou J.J., Fernandez E., Forde B.G., Miller A.J.;
RT "Functional Expression and kinetic Characterisation of Nar2 gene of
RT Arabidopsis in Xenopus oocyte.";
RL Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP MUTAGENESIS OF ASP-105.
RX PubMed=16980702; DOI=10.1093/pcp/pcl010;
RA Kawachi T., Sunaga Y., Ebato M., Hatanaka T., Harada H.;
RT "Repression of nitrate uptake by replacement of Asp105 by asparagine in
RT AtNRT3.1 in Arabidopsis thaliana L.";
RL Plant Cell Physiol. 47:1437-1441(2006).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION BY NITRATE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=16415212; DOI=10.1104/pp.105.074385;
RA Okamoto M., Kumar A., Li W., Wang Y., Siddiqi M.Y., Crawford N.M.,
RA Glass A.D.;
RT "High-affinity nitrate transport in roots of Arabidopsis depends on
RT expression of the NAR2-like gene AtNRT3.1.";
RL Plant Physiol. 140:1036-1046(2006).
RN [7]
RP FUNCTION, INTERACTION WITH NRT2.1 AND NRT2.3, INDUCTION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=17012411; DOI=10.1104/pp.106.085209;
RA Orsel M., Chopin F., Leleu O., Smith S.J., Krapp A., Daniel-Vedele F.,
RA Miller A.J.;
RT "Characterization of a two-component high-affinity nitrate uptake system in
RT Arabidopsis. Physiology and protein-protein interaction.";
RL Plant Physiol. 142:1304-1317(2006).
RN [8]
RP FUNCTION.
RX PubMed=17573350; DOI=10.1074/jbc.m700901200;
RA Wirth J., Chopin F., Santoni V., Viennois G., Tillard P., Krapp A.,
RA Lejay L., Daniel-Vedele F., Gojon A.;
RT "Regulation of root nitrate uptake at the NRT2.1 protein level in
RT Arabidopsis thaliana.";
RL J. Biol. Chem. 282:23541-23552(2007).
RN [9]
RP FUNCTION.
RX PubMed=19704673; DOI=10.4161/psb.2.4.3870;
RA Orsel M., Chopin F., Leleu O., Smith S.J., Krapp A., Daniel-Vedele F.,
RA Miller A.J.;
RT "Nitrate signaling and the two component high affinity uptake system in
RT Arabidopsis.";
RL Plant Signal. Behav. 2:260-262(2007).
RN [10]
RP SUBCELLULAR LOCATION, SUBUNIT, AND INTERACTION WITH NRT2.1.
RX PubMed=20561257; DOI=10.1111/j.1365-313x.2010.04278.x;
RA Yong Z., Kotur Z., Glass A.D.;
RT "Characterization of an intact two-component high-affinity nitrate
RT transporter from Arabidopsis roots.";
RL Plant J. 63:739-748(2010).
RN [11]
RP INTERACTION WITH NRT2 TRANSPORTERS.
RX PubMed=22432443; DOI=10.1111/j.1469-8137.2012.04094.x;
RA Kotur Z., Mackenzie N., Ramesh S., Tyerman S.D., Kaiser B.N., Glass A.D.;
RT "Nitrate transport capacity of the Arabidopsis thaliana NRT2 family members
RT and their interactions with AtNAR2.1.";
RL New Phytol. 194:724-731(2012).
CC -!- FUNCTION: Acts as a dual component transporter with NTR2.1. Required
CC for high-affinity nitrate transport. Acts as a repressor of lateral
CC root initiation. May be involved in targeting NRT2 proteins to the
CC plasma membrane. {ECO:0000269|PubMed:16415212,
CC ECO:0000269|PubMed:17012411, ECO:0000269|PubMed:17573350,
CC ECO:0000269|PubMed:19704673}.
CC -!- SUBUNIT: Heterotetramer composed of two NRT2.1 and two NRT3.1
CC (PubMed:20561257). Interacts with NRT2.1 and NRT2.3 (PubMed:17012411).
CC Interacts with all other NRT2 transporters, including NRT2.5
CC (PubMed:22432443). {ECO:0000269|PubMed:17012411,
CC ECO:0000269|PubMed:20561257, ECO:0000269|PubMed:22432443}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20561257};
CC Single-pass membrane protein {ECO:0000269|PubMed:20561257}.
CC -!- TISSUE SPECIFICITY: Highly expressed in roots. Detected in shoots.
CC {ECO:0000269|PubMed:16415212}.
CC -!- INDUCTION: Up-regulated in roots by nitrate. No effect in shoots.
CC Induced by sudden N starvation and by low nitrate concentration.
CC {ECO:0000269|PubMed:16415212, ECO:0000269|PubMed:17012411}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype when grown under normal
CC conditions. Reduced growth on low-nitrate media.
CC {ECO:0000269|PubMed:16415212, ECO:0000269|PubMed:17012411}.
CC -!- SIMILARITY: Belongs to the NAR2 family. {ECO:0000305}.
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DR EMBL; AJ310933; CAC36942.1; -; mRNA.
DR EMBL; AJ311926; CAC36292.1; -; mRNA.
DR EMBL; AB024031; BAB09391.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95909.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95910.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95911.1; -; Genomic_DNA.
DR EMBL; AY085586; AAM62807.1; -; mRNA.
DR RefSeq; NP_199831.1; NM_124399.3.
DR RefSeq; NP_851159.1; NM_180828.2.
DR RefSeq; NP_851160.1; NM_180829.1.
DR AlphaFoldDB; Q9FGS5; -.
DR BioGRID; 20331; 6.
DR IntAct; Q9FGS5; 3.
DR STRING; 3702.AT5G50200.2; -.
DR TCDB; 8.A.20.1.1; the plant/algal/chlorella nitrate transporter accessory protein (nar2,1) family.
DR PaxDb; Q9FGS5; -.
DR PRIDE; Q9FGS5; -.
DR ProteomicsDB; 250572; -.
DR EnsemblPlants; AT5G50200.1; AT5G50200.1; AT5G50200.
DR EnsemblPlants; AT5G50200.2; AT5G50200.2; AT5G50200.
DR EnsemblPlants; AT5G50200.3; AT5G50200.3; AT5G50200.
DR GeneID; 835085; -.
DR Gramene; AT5G50200.1; AT5G50200.1; AT5G50200.
DR Gramene; AT5G50200.2; AT5G50200.2; AT5G50200.
DR Gramene; AT5G50200.3; AT5G50200.3; AT5G50200.
DR KEGG; ath:AT5G50200; -.
DR Araport; AT5G50200; -.
DR TAIR; locus:2157737; AT5G50200.
DR eggNOG; ENOG502RXTZ; Eukaryota.
DR HOGENOM; CLU_093989_0_0_1; -.
DR InParanoid; Q9FGS5; -.
DR OrthoDB; 1249242at2759; -.
DR PhylomeDB; Q9FGS5; -.
DR PRO; PR:Q9FGS5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FGS5; baseline and differential.
DR Genevisible; Q9FGS5; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IMP:TAIR.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IMP:TAIR.
DR GO; GO:0010167; P:response to nitrate; IEP:TAIR.
DR GO; GO:0009611; P:response to wounding; IEP:TAIR.
DR InterPro; IPR016605; Transptr_NO3_Nar2.
DR PANTHER; PTHR34806; PTHR34806; 1.
DR Pfam; PF16974; NAR2; 1.
DR PIRSF; PIRSF012939; Transpt_NO3_Nar2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Nitrate assimilation; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..210
FT /note="High-affinity nitrate transporter 3.1"
FT /id="PRO_0000400105"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 105
FT /note="D->N: In rnc1; decreased nitrate uptake and
FT accumulation."
FT /evidence="ECO:0000269|PubMed:16980702"
FT CONFLICT 26
FT /note="V -> L (in Ref. 1; CAC36942 and 4; AAM62807)"
FT /evidence="ECO:0000305"
FT CONFLICT 102
FT /note="L -> F (in Ref. 4; AAM62807)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 210 AA; 23403 MW; 5E82A99EE721508E CRC64;
MAIQKILFAS LLICSLIQSI HGAEKVRLFK ELDKGALDVT TKPSREGPGV VLDAGKDTLN
ITWTLSSIGS KREAEFKIIK VKLCYAPPSQ VDRPWRKTHD ELFKDKTCPH KIIAKPYDKT
LQSTTWTLER DIPTGTYFVR AYAVDAIGHE VAYGQSTDDA KKTNLFSVQA ISGRHASLDI
ASICFSVFSV VALVVFFVNE KRKAKIEQSK
